PMID- 35216636
OWN - NLM
STAT- MEDLINE
DCOM- 20220301
LR  - 20220531
IS  - 1916-0216 (Electronic)
IS  - 1916-0208 (Print)
IS  - 1916-0208 (Linking)
VI  - 51
IP  - 1
DP  - 2022 Feb 25
TI  - LncRNA CASC15 upregulates cyclin D1 by downregulating miR-365 in laryngeal 
      squamous cell carcinoma to promote cell proliferation.
PG  - 8
LID - 10.1186/s40463-022-00560-2 [doi]
LID - 8
AB  - BACKGROUND: This study investigated the role of lncRNA CASC15 in laryngeal 
      squamous cell carcinoma (LSCC). METHODS: This study included 58 LSCC patients. 
      Both tumor (LSCC) and adjacent (within 3 cm around tumors) non-tumor tissues from 
      3 different sites of each patient were collected. CCK-8 assay was used to 
      determine cell proliferation. The expression levels of proteins and mRNAs were 
      determined by Western blotting analysis and qRT-PCRs, respectively. RESULTS: 
      CASC15 was upregulated in LSCC and high expression levels of CASC15 predicted 
      poor survival. In LSCC tissues, CASC15 was negatively correlated with miR-365 but 
      positively correlated with cyclin D1. In LSCC cells, overexpression of CASC15 
      resulted in downregulation of miR-365 and upregulation of cyclin D1. 
      Overexpression of miR-365 did not affect the expression of CASC15 but 
      downregulated cyclin D1. Overexpression of Cyclin D1 did not affect the 
      expression of miR-365 and CASC15. Overexpression of CASC15 and cyclin D1 led to 
      promoted, while overexpression of miR-365 led to inhibited LSCC cell 
      proliferation. In addition, overexpression of miR-365 reduced the effects of 
      overexpression of CASC15. CONCLUSION: Therefore, CASC15 upregulates cyclin D1 by 
      downregulating miR-365 in LSCC to promote cell proliferation.
CI  - (c) 2022. The Author(s).
FAU - Zhu, Minhui
AU  - Zhu M
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Changhai Hospital, Navy 
      Military Medical University, No. 168 Changhai Road, Shanghai, 200433, People's 
      Republic of China.
FAU - Zhang, Caiyun
AU  - Zhang C
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Changhai Hospital, Navy 
      Military Medical University, No. 168 Changhai Road, Shanghai, 200433, People's 
      Republic of China.
FAU - Zhou, Peng
AU  - Zhou P
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Changhai Hospital, Navy 
      Military Medical University, No. 168 Changhai Road, Shanghai, 200433, People's 
      Republic of China.
FAU - Chen, Shicai
AU  - Chen S
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Changhai Hospital, Navy 
      Military Medical University, No. 168 Changhai Road, Shanghai, 200433, People's 
      Republic of China. yf5909@163.com.
FAU - Zheng, Hongliang
AU  - Zheng H
AUID- ORCID: 0000-0001-9359-1935
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Changhai Hospital, Navy 
      Military Medical University, No. 168 Changhai Road, Shanghai, 200433, People's 
      Republic of China. hongliangzhengmedi@163.com.
LA  - eng
GR  - 81772881/National Natural Science Foundation of China/
GR  - 81572668/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20220225
PL  - England
TA  - J Otolaryngol Head Neck Surg
JT  - Journal of otolaryngology - head & neck surgery = Le Journal 
      d'oto-rhino-laryngologie et de chirurgie cervico-faciale
JID - 101479544
RN  - 0 (CCND1 protein, human)
RN  - 0 (MIRN365 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - 136601-57-5 (Cyclin D1)
SB  - IM
MH  - *Carcinoma, Squamous Cell/pathology
MH  - Cell Proliferation/genetics
MH  - Cyclin D1/genetics/metabolism
MH  - Gene Expression Regulation, Neoplastic
MH  - *Head and Neck Neoplasms/genetics
MH  - Humans
MH  - *Laryngeal Neoplasms/pathology
MH  - *MicroRNAs/genetics
MH  - *RNA, Long Noncoding/genetics/metabolism
MH  - Squamous Cell Carcinoma of Head and Neck/genetics
MH  - Up-Regulation
PMC - PMC8881844
OTO - NOTNLM
OT  - Cyclin D1
OT  - Laryngeal squamous cell carcinoma
OT  - lncRNA CASC15
OT  - miR-365
COIS- All other authors have no competing interests. We declare that we do not have any 
      commercial or associative interest that represents a conflict of interest in 
      connection with the work submitted.
EDAT- 2022/02/27 06:00
MHDA- 2022/03/03 06:00
PMCR- 2022/02/25
CRDT- 2022/02/26 05:30
PHST- 2021/08/19 00:00 [received]
PHST- 2022/01/31 00:00 [accepted]
PHST- 2022/02/26 05:30 [entrez]
PHST- 2022/02/27 06:00 [pubmed]
PHST- 2022/03/03 06:00 [medline]
PHST- 2022/02/25 00:00 [pmc-release]
AID - 10.1186/s40463-022-00560-2 [pii]
AID - 560 [pii]
AID - 10.1186/s40463-022-00560-2 [doi]
PST - epublish
SO  - J Otolaryngol Head Neck Surg. 2022 Feb 25;51(1):8. doi: 
      10.1186/s40463-022-00560-2.