PMID- 35217063
OWN - NLM
STAT- MEDLINE
DCOM- 20220317
LR  - 20220317
IS  - 1095-6840 (Electronic)
IS  - 0016-6480 (Linking)
VI  - 320
DP  - 2022 May 1
TI  - Changes in gene expression in rat placenta at gestational day 16.5 in response to 
      hyperglycemia.
PG  - 113999
LID - S0016-6480(22)00024-7 [pii]
LID - 10.1016/j.ygcen.2022.113999 [doi]
AB  - Gestational diabetes mellitus (GDM) is a serious pregnancy complication. 
      Hyperglycemia induces abnormal placental development and function. However, the 
      mechanism is unclear. Previous research showed streptozocin (STZ) injection 
      sustained hyperglycemia throughout pregnancy in rodents. Our current results 
      showed that the placenta from hyperglycemic STZ-treated rats was about 20% 
      heavier than that of controls. The relative thickness of each layer of the 
      placenta was also significantly different on gestational day (GD) 16.5. Gene 
      expression was analyzed by RNA sequencing to explore reasons for the abnormal 
      placenta. In total, 2100 differential expressed genes (DEGs), including 1327 
      up-regulated and 773 down-regulated genes, were identified. Gene ontogeny (GO) 
      analysis revealed DEGs involved in developmental process, growth, metabolic 
      process, cell junction, molecular transducer activity and signaling. By KEGG 
      analysis, DEGs were mainly related to the endocrine system, development, signal 
      transduction and cell growth and death. The KEGG results were partly consistent 
      with GO results, with DEGs mainly focused on biochemical signal pathways such as 
      cell growth and death (e.g., Abl1, Bbc3 and Camk2d), and signal transduction 
      (e.g., Abl1, Ceacam1 and Arnt). These genes may play a dominant role in abnormal 
      cell proliferation and signaling disorders. These results suggest that DEGs play 
      a role in diabetic-induced placental abnormalities. One or more of these DEGs may 
      be involved in the etiology of placental weight increase caused by hyperglycemia.
CI  - Copyright (c) 2022 Elsevier Inc. All rights reserved.
FAU - Meng, Rui
AU  - Meng R
AD  - Graduate School of Peking Union Medical College, Chinese Academy of Medical 
      Sciences, Beijing 100730, China; Department of Genetics, National Research 
      Institute for Family Planning, Health Department, Beijing 100081, China.
FAU - Gao, Qianqian
AU  - Gao Q
AD  - Laboratory of Novel Pharmaceutical Excipients, Sustained and Controlled Release 
      Preparations, College of Medicine and Nursing, Dezhou University, Dezhou 253023, 
      China.
FAU - Liang, Ranran
AU  - Liang R
AD  - College of Life Science, Dezhou University, Dezhou, Shandong, China.
FAU - Guan, Lina
AU  - Guan L
AD  - Graduate School of Peking Union Medical College, Chinese Academy of Medical 
      Sciences, Beijing 100730, China.
FAU - Liu, Shanhe
AU  - Liu S
AD  - Mudanjiang Medical College, Mudanjiang, Heilongjiang, China.
FAU - Zhu, Qinghua
AU  - Zhu Q
AD  - College of Life Science, Dezhou University, Dezhou, Shandong, China.
FAU - Su, Dongmei
AU  - Su D
AD  - Graduate School of Peking Union Medical College, Chinese Academy of Medical 
      Sciences, Beijing 100730, China; Department of Genetics, National Research 
      Institute for Family Planning, Health Department, Beijing 100081, China. 
      Electronic address: sudongmeisdm@126.com.
FAU - Xia, Yixin
AU  - Xia Y
AD  - Obstetrics and Gynecology, Peking University Shougang Hospital,Beijing, China. 
      Electronic address: xinyibj@126.com.
FAU - Ma, Xu
AU  - Ma X
AD  - Graduate School of Peking Union Medical College, Chinese Academy of Medical 
      Sciences, Beijing 100730, China; Department of Genetics, National Research 
      Institute for Family Planning, Health Department, Beijing 100081, China. 
      Electronic address: jswkysgc@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220222
PL  - United States
TA  - Gen Comp Endocrinol
JT  - General and comparative endocrinology
JID - 0370735
SB  - IM
MH  - Animals
MH  - *Diabetes, Gestational/genetics/metabolism
MH  - Female
MH  - Gene Expression
MH  - *Hyperglycemia/metabolism
MH  - Placenta/metabolism
MH  - Pregnancy
MH  - Rats
MH  - Signal Transduction
OTO - NOTNLM
OT  - Differential expression genes
OT  - Hyperglycemia
OT  - Rat placenta
OT  - Transcriptome sequencing
EDAT- 2022/02/27 06:00
MHDA- 2022/03/18 06:00
CRDT- 2022/02/26 05:33
PHST- 2021/11/25 00:00 [received]
PHST- 2022/02/11 00:00 [revised]
PHST- 2022/02/15 00:00 [accepted]
PHST- 2022/02/27 06:00 [pubmed]
PHST- 2022/03/18 06:00 [medline]
PHST- 2022/02/26 05:33 [entrez]
AID - S0016-6480(22)00024-7 [pii]
AID - 10.1016/j.ygcen.2022.113999 [doi]
PST - ppublish
SO  - Gen Comp Endocrinol. 2022 May 1;320:113999. doi: 10.1016/j.ygcen.2022.113999. 
      Epub 2022 Feb 22.