PMID- 35218517
OWN - NLM
STAT- MEDLINE
DCOM- 20220708
LR  - 20220714
IS  - 1559-0305 (Electronic)
IS  - 1073-6085 (Linking)
VI  - 64
IP  - 8
DP  - 2022 Aug
TI  - LncRNA NCK1-AS1 Promotes Malignant Cellular Phenotypes of Laryngeal Squamous Cell 
      Carcinoma via miR-137/NCK1 Axis.
PG  - 888-901
LID - 10.1007/s12033-022-00469-1 [doi]
AB  - Increasing evidence demonstrates that many long noncoding RNAs (lncRNAs) are 
      implicated with the development of laryngeal squamous cell carcinoma (LSCC). As 
      shown by bioinformatics analysis, lncRNA non-catalytic region of tyrosine kinase 
      adaptor protein 1-antisense 1 (NCK1-AS1) is upregulated in tissues of head and 
      neck squamous cell carcinoma. The study aimed to explore the role and mechanism 
      of NCK1-AS1 in LSCC. NCK1-AS1 expression in LSCC cells was evaluated by reverse 
      transcription qPCR. The viability, proliferation, invasion, migration, and 
      apoptosis of LSCC cells with indicated transfection were evaluated by CCK-8 
      assays, Ethynyl deoxyuridine incorporation assays, Transwell assays, wound 
      healing assays, and TUNEL assays, respectively. Subcellular fractionation assays 
      were performed to evaluate the cellular distribution of NCK1-AS1 and NCK1. NCK1 
      protein level in LSCC cells with indicated transfection was quantified by western 
      blotting. The binding relation between miR-137 and NCK1-AS1 (or NCK1) were 
      determined using RNA immunoprecipitation assays and luciferase reporter assays. 
      NCK1-AS1 was highly expressed in LSCC cell lines. NCK1-AS1 depletion suppressed 
      LSCC cell viability, proliferation, invasion, and migration while enhancing cell 
      apoptosis. NCK1, an adjacent gene of NCK1-AS1, is also highly expressed in LSCC 
      cells and was positively regulated by NCK1-AS1. Moreover, NCK1-AS1 interact with 
      miR-137 to upregulate NCK1 expression. NCK1 was the downstream target of miR-137 
      and was negatively correlated to miR-137. In addition, overexpressed NCK1 
      reversed the suppressive impact of NCK1-AS1 depletion on malignant behaviors of 
      LSCC cells. NCK1-AS1 contributes to LSCC cellular behaviors by upregulating NCK1 
      via interaction with miR-137.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Wan, Lanlan
AU  - Wan L
AD  - Department of Otolaryngology, The Affiliated Huaian No.1 People's Hospital of 
      Nanjing Medical University, No. 6, Beijing West Road, Huaian, 223300, Jiangsu, 
      China.
FAU - Gu, Dongsheng
AU  - Gu D
AD  - Department of Otolaryngology, The Affiliated Huaian No.1 People's Hospital of 
      Nanjing Medical University, No. 6, Beijing West Road, Huaian, 223300, Jiangsu, 
      China.
FAU - Jin, Xin
AU  - Jin X
AUID- ORCID: 0000-0003-0800-6434
AD  - Department of Otolaryngology, The Affiliated Huaian No.1 People's Hospital of 
      Nanjing Medical University, No. 6, Beijing West Road, Huaian, 223300, Jiangsu, 
      China. jinxin6996@hotmail.com.
LA  - eng
PT  - Journal Article
DEP - 20220226
PL  - Switzerland
TA  - Mol Biotechnol
JT  - Molecular biotechnology
JID - 9423533
RN  - 0 (MIRN137 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
SB  - IM
EIN - Mol Biotechnol. 2022 Mar 22;:. PMID: 35316472
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Gene Expression Regulation, Neoplastic
MH  - *Head and Neck Neoplasms/genetics
MH  - Humans
MH  - *MicroRNAs/genetics/metabolism
MH  - Phenotype
MH  - *RNA, Long Noncoding/genetics/metabolism
MH  - Squamous Cell Carcinoma of Head and Neck/genetics
OTO - NOTNLM
OT  - Laryngeal squamous cell carcinoma
OT  - NCK1
OT  - NCK1-AS1
OT  - ceRNA
OT  - miR-137
EDAT- 2022/02/27 06:00
MHDA- 2022/07/09 06:00
CRDT- 2022/02/26 12:07
PHST- 2021/11/17 00:00 [received]
PHST- 2022/02/12 00:00 [accepted]
PHST- 2022/02/27 06:00 [pubmed]
PHST- 2022/07/09 06:00 [medline]
PHST- 2022/02/26 12:07 [entrez]
AID - 10.1007/s12033-022-00469-1 [pii]
AID - 10.1007/s12033-022-00469-1 [doi]
PST - ppublish
SO  - Mol Biotechnol. 2022 Aug;64(8):888-901. doi: 10.1007/s12033-022-00469-1. Epub 
      2022 Feb 26.