PMID- 35218738
OWN - NLM
STAT- MEDLINE
DCOM- 20220408
LR  - 20230612
IS  - 1542-0086 (Electronic)
IS  - 0006-3495 (Print)
IS  - 0006-3495 (Linking)
VI  - 121
IP  - 7
DP  - 2022 Apr 5
TI  - Effect of cholesterol on the lactosylceramide domains in phospholipid bilayers.
PG  - 1143-1155
LID - S0006-3495(22)00186-2 [pii]
LID - 10.1016/j.bpj.2022.02.037 [doi]
AB  - Lactosylceramide (LacCer) in the plasma membranes of immune cells is an important 
      lipid for signaling in innate immunity through the formation of LacCer-rich 
      domains together with cholesterol (Cho). However, the properties of the LacCer 
      domains formed in multicomponent membranes remain unclear. In this study, we 
      examined the properties of the LacCer domains formed in Cho-containing 
      1-palmitoyl-2-oleoyl phosphatidylcholine (POPC) membranes by deuterium 
      solid-state NMR and fluorescence lifetimes. The potent affinity of LacCer-LacCer 
      (homophilic interaction) is known to induce a thermally stable gel phase in the 
      unitary LacCer bilayer. In LacCer/Cho binary membranes, Cho gradually 
      destabilized the LacCer gel phase to form the liquid-ordered phase by its potent 
      order effect. In the LacCer/POPC binary systems without Cho, the (2)H NMR spectra 
      of 10',10'-d(2)-LacCer and 18',18',18'-d(3)-LacCer probes revealed that LacCer 
      was poorly miscible with POPC in the membranes and formed stable gel phases 
      without being distributed in the liquid crystalline domain. The lamellar 
      structure of the LacCer/POPC membrane was gradually disrupted at around 60 degrees C, 
      whereas the addition of Cho increased the thermal stability of the lamellarity. 
      Furthermore, the area of the LacCer gel phase and its chain order were decreased 
      in the LacCer/POPC/Cho ternary membranes, whereas the liquid-ordered domain, 
      which was observed in the LacCer/Cho binary membrane, was not observed. Cho 
      surrounding the LacCer gel domain liberated LacCer and facilitated forming the 
      submicron to nano-scale small domains in the liquid crystalline domain of the 
      LacCer/POPC/Cho membranes, as revealed by the fluorescence lifetimes of 
      trans-parinaric acid and trans-parinaric acid-LacCer. Our findings on the 
      membrane properties of the LacCer domains, particularly in the presence of Cho, 
      would help elucidate the properties of the LacCer domains in biological 
      membranes.
CI  - Copyright (c) 2022 Biophysical Society. Published by Elsevier Inc. All rights 
      reserved.
FAU - Hanashima, Shinya
AU  - Hanashima S
AD  - Graduate School of Science, Osaka University, Toyonaka, Osaka, Japan. Electronic 
      address: hanashimas13@chem.sci.osaka-u.ac.jp.
FAU - Ikeda, Ryuji
AU  - Ikeda R
AD  - Graduate School of Science, Osaka University, Toyonaka, Osaka, Japan.
FAU - Matsubara, Yuki
AU  - Matsubara Y
AD  - Graduate School of Science, Osaka University, Toyonaka, Osaka, Japan.
FAU - Yasuda, Tomokazu
AU  - Yasuda T
AD  - Graduate School of Science, Osaka University, Toyonaka, Osaka, Japan.
FAU - Tsuchikawa, Hiroshi
AU  - Tsuchikawa H
AD  - Graduate School of Science, Osaka University, Toyonaka, Osaka, Japan.
FAU - Slotte, J Peter
AU  - Slotte JP
AD  - Biochemistry, Faculty of Science and Engineering, Abo Akademi University, Turku, 
      Finland.
FAU - Murata, Michio
AU  - Murata M
AD  - Graduate School of Science, Osaka University, Toyonaka, Osaka, Japan; JST ERATO, 
      Lipid Active Structure Project, Osaka University, Toyonaka, Osaka, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220223
PL  - United States
TA  - Biophys J
JT  - Biophysical journal
JID - 0370626
RN  - 0 (Antigens, CD)
RN  - 0 (Lactosylceramides)
RN  - 0 (Lipid Bilayers)
RN  - 0 (Phosphatidylcholines)
RN  - 0 (Phospholipids)
RN  - 4682-48-8 (CDw17 antigen)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - Antigens, CD
MH  - Cholesterol/chemistry
MH  - Lactosylceramides
MH  - Lipid Bilayers/chemistry
MH  - *Phosphatidylcholines/chemistry
MH  - *Phospholipids/chemistry
PMC - PMC9034317
EDAT- 2022/02/27 06:00
MHDA- 2022/04/09 06:00
PMCR- 2023/04/05
CRDT- 2022/02/26 20:10
PHST- 2021/08/31 00:00 [received]
PHST- 2021/10/22 00:00 [revised]
PHST- 2022/02/22 00:00 [accepted]
PHST- 2022/02/27 06:00 [pubmed]
PHST- 2022/04/09 06:00 [medline]
PHST- 2022/02/26 20:10 [entrez]
PHST- 2023/04/05 00:00 [pmc-release]
AID - S0006-3495(22)00186-2 [pii]
AID - 10.1016/j.bpj.2022.02.037 [doi]
PST - ppublish
SO  - Biophys J. 2022 Apr 5;121(7):1143-1155. doi: 10.1016/j.bpj.2022.02.037. Epub 2022 
      Feb 23.