PMID- 35220548
OWN - NLM
STAT- MEDLINE
DCOM- 20220411
LR  - 20220411
IS  - 1573-4919 (Electronic)
IS  - 0300-8177 (Linking)
VI  - 477
IP  - 5
DP  - 2022 May
TI  - Effect of hyperhomocysteinemia on rat cardiac sarcoplasmic reticulum.
PG  - 1621-1628
LID - 10.1007/s11010-022-04399-z [doi]
AB  - Increased concentration of plasma homocysteine (Hcy) is an independent risk 
      factor of cardiovascular disease, yet the mechanism by which hyperhomocysteinemia 
      (HHcy) causes cardiac dysfunction is largely unknown. The aim of present study 
      was to investigate the contribution of sarcoplasmic reticulum to impaired cardiac 
      contractile function in HHCy. HHcy-induced by subcutaneous injection of Hcy 
      (0.45 mumol/g of body weight) twice a day for a period of 2 weeks resulted in 
      significant decrease in developed left ventricular pressure and maximum rate of 
      ventricular relaxation. Our results show that abundances of SR Ca(2+)-handling 
      proteins, Ca(2+)-ATPase (SERCA2), calsequestrin and histidine-rich 
      calcium-binding protein are significantly reduced while the content of 
      phospholamban is unchanged. Moreover, we found that increased PLN:SERCA2 ratio 
      results in the inhibition of SERCA2 activity at low free Ca(2+) concentrations. 
      We further discovered that HHcy is not associated with increased oxidative stress 
      in SR. Taken together, these findings suggest that disturbances in SR Ca(2+) 
      handling, caused by altered protein contents but not oxidative damage, may 
      contribute to impaired cardiac contractility in HHcy.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Tatarkova, Zuzana
AU  - Tatarkova Z
AD  - Department of Medical Biochemistry, Jessenius Faculty of Medicine, Comenius 
      University in Bratislava, Mala Hora 4D, 036 01, Martin, Slovakia.
FAU - Bencurova, Maria
AU  - Bencurova M
AD  - Department of Medical Biochemistry, Jessenius Faculty of Medicine, Comenius 
      University in Bratislava, Mala Hora 4D, 036 01, Martin, Slovakia.
FAU - Lehotsky, Jan
AU  - Lehotsky J
AD  - Department of Medical Biochemistry, Jessenius Faculty of Medicine, Comenius 
      University in Bratislava, Mala Hora 4D, 036 01, Martin, Slovakia.
FAU - Racay, Peter
AU  - Racay P
AD  - Department of Medical Biochemistry, Jessenius Faculty of Medicine, Comenius 
      University in Bratislava, Mala Hora 4D, 036 01, Martin, Slovakia.
FAU - Kmetova Sivonova, Monika
AU  - Kmetova Sivonova M
AD  - Department of Medical Biochemistry, Jessenius Faculty of Medicine, Comenius 
      University in Bratislava, Mala Hora 4D, 036 01, Martin, Slovakia.
FAU - Dobrota, Dusan
AU  - Dobrota D
AD  - Department of Medical Biochemistry, Jessenius Faculty of Medicine, Comenius 
      University in Bratislava, Mala Hora 4D, 036 01, Martin, Slovakia.
FAU - Kaplan, Peter
AU  - Kaplan P
AUID- ORCID: 0000-0002-9299-4190
AD  - Department of Medical Biochemistry, Jessenius Faculty of Medicine, Comenius 
      University in Bratislava, Mala Hora 4D, 036 01, Martin, Slovakia. 
      peter.kaplan@uniba.sk.
LA  - eng
GR  - 1/0004/19/Vedecka Grantova Agentura MSVVaS SR a SAV/
PT  - Journal Article
DEP - 20220227
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Calsequestrin)
RN  - EC 3.6.3.8 (Sarcoplasmic Reticulum Calcium-Transporting ATPases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/metabolism
MH  - Calcium-Binding Proteins/metabolism
MH  - Calsequestrin/metabolism
MH  - Heart/physiology
MH  - *Hyperhomocysteinemia/chemically induced
MH  - Myocardial Contraction
MH  - Myocardium/metabolism
MH  - Rats
MH  - *Sarcoplasmic Reticulum/metabolism
MH  - Sarcoplasmic Reticulum Calcium-Transporting ATPases
OTO - NOTNLM
OT  - Ca2+-ATPase
OT  - Heart
OT  - Homocysteine
OT  - Protein expression
OT  - Sarcoplasmic reticulum
EDAT- 2022/02/28 06:00
MHDA- 2022/04/12 06:00
CRDT- 2022/02/27 20:38
PHST- 2021/11/30 00:00 [received]
PHST- 2022/02/16 00:00 [accepted]
PHST- 2022/02/28 06:00 [pubmed]
PHST- 2022/04/12 06:00 [medline]
PHST- 2022/02/27 20:38 [entrez]
AID - 10.1007/s11010-022-04399-z [pii]
AID - 10.1007/s11010-022-04399-z [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2022 May;477(5):1621-1628. doi: 10.1007/s11010-022-04399-z. 
      Epub 2022 Feb 27.