PMID- 35220878
OWN - NLM
STAT- MEDLINE
DCOM- 20220405
LR  - 20220405
IS  - 2165-5987 (Electronic)
IS  - 2165-5979 (Print)
IS  - 2165-5979 (Linking)
VI  - 13
IP  - 3
DP  - 2022 Mar
TI  - Long noncoding RNA endogenous bornavirus-like nucleoprotein acts as an oncogene 
      by regulating microRNA-655-3p expression in T-cell acute lymphoblastic leukemia.
PG  - 6409-6419
LID - 10.1080/21655979.2022.2044249 [doi]
AB  - Acute lymphocytic leukemia (ALL) is the most common malignant tumor in children 
      with T-cell ALL (T-ALL), accounting for approximately 15% of all cases. Long 
      noncoding RNAs (lncRNAs) are involved in the pathogenesis and progression of 
      T-ALL. The present study aimed to explore the role and mechanism of action of 
      lncRNA EBLN3P in T-ALL. We used quantitative reverse transcription-PCR (qRT-PCR) 
      to determine the expression of lncRNA endogenous bornavirus-like nucleoprotein 
      (EBLN3P), microRNA (miR)-655-3p, and the transcription level of matrix 
      metalloproteinase-9 (MMP-9), and Western blot assay to quantify the protein 
      expression level of cleaved-caspase3, caspase3, proliferating cell nuclear 
      antigen (PCNA), and MMP-9. The potential binding sites between lncRNA EBLN3P and 
      miR-655-3p were predicted using StarBase, and the interaction was further 
      verified by dual-luciferase reporter assay and RNA pull-down assay. The 
      proliferation ability of Jurkat cells was detected using MTT 
      (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, and their 
      invasion and migration ability using transwell assay. Cell apoptosis was 
      determined using flow cytometry (FCM) assay. The expression of lncRNA EBLN3P was 
      upregulated while that of miR-655-3p was downregulated in human T-ALL cell lines 
      and lncRNA EBLN3P negatively regulated miR-655-3p. LncRNA EBLN3P knockdown 
      significantly inhibited proliferation, invasion, and migration of Jurkat cells 
      and induced their apoptosis. Downregulating miR-655-3p reversed the effects of 
      lncRNA EBLN3P knockdown on Jurkat cells. In conclusion, we confirmed for the 
      first time that lncRNA EBLN3P is dysregulated in T-ALL cell lines, and lncRNA 
      EBLN3P knockdown inhibited the malignant biological behaviors of T-ALL cells by 
      up-regulating miR-655-3p.
FAU - Yang, Jinhua
AU  - Yang J
AD  - Department of Hematology, Wuhan Fourth Hospital, Puai Hospital, Tongji Medical 
      College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
FAU - Yang, Yingying
AU  - Yang Y
AD  - Department of Hematology, Wuhan Fourth Hospital, Puai Hospital, Tongji Medical 
      College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Bioengineered
JT  - Bioengineered
JID - 101581063
RN  - 0 (MIRN655 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
SB  - IM
MH  - Apoptosis/genetics
MH  - Humans
MH  - Jurkat Cells
MH  - MicroRNAs/*genetics
MH  - Oncogenes/*genetics
MH  - *Precursor T-Cell Lymphoblastic Leukemia-Lymphoma/genetics/metabolism
MH  - RNA, Long Noncoding/*genetics
PMC - PMC8974199
OTO - NOTNLM
OT  - T-ALL
OT  - lncRNA EBLN3P
OT  - metastasis
OT  - miR-655-3p
OT  - tumor invasion
OT  - tumor proliferation
COIS- No potential conflict of interest was reported by the author(s).
EDAT- 2022/03/01 06:00
MHDA- 2022/04/06 06:00
PMCR- 2022/02/27
CRDT- 2022/02/28 05:27
PHST- 2022/02/28 05:27 [entrez]
PHST- 2022/03/01 06:00 [pubmed]
PHST- 2022/04/06 06:00 [medline]
PHST- 2022/02/27 00:00 [pmc-release]
AID - 2044249 [pii]
AID - 10.1080/21655979.2022.2044249 [doi]
PST - ppublish
SO  - Bioengineered. 2022 Mar;13(3):6409-6419. doi: 10.1080/21655979.2022.2044249.