PMID- 35223986
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220301
IS  - 2296-889X (Print)
IS  - 2296-889X (Electronic)
IS  - 2296-889X (Linking)
VI  - 8
DP  - 2021
TI  - SERS Based Lateral Flow Assay for Rapid and Ultrasensitive Quantification of Dual 
      Laryngeal Squamous Cell Carcinoma-Related miRNA Biomarkers in Human Serum Using 
      Pd-Au Core-Shell Nanorods and Catalytic Hairpin Assembly.
PG  - 813007
LID - 10.3389/fmolb.2021.813007 [doi]
LID - 813007
AB  - Non-invasive early diagnosis is of great significant in disease pathologic 
      development and subsequent medical treatments, and microRNA (miRNA) detection has 
      attracted critical attention in early cancer screening and diagnosis. However, it 
      was still a challenge to report an accurate and sensitive method for the 
      detection of miRNA during cancer development, especially in the presence of its 
      analogs that produce intense background noise. Herein, we developed a 
      surface-enhanced Raman scattering (SERS)-based lateral flow assay (LFA) 
      biosensor, assisted with catalytic hairpin assembly (CHA) amplification strategy, 
      for the dynamic monitoring of miR-106b and miR-196b, associated with laryngeal 
      squamous cell carcinoma (LSCC). In the presence of target miRNAs, two hairpin 
      DNAs could self-assemble into double-stranded DNA, exposing the biotin molecules 
      modified on the surface of palladium (Pd)-gold (Au) core-shell nanorods 
      (Pd-AuNRs). Then, the biotin molecules could be captured by the streptavidin 
      (SA), which was fixed on the test lines (T1 line and T2 line) beforehand. The 
      core-shell spatial structures and aggregation Pd-AuNRs generated abundant active 
      "hot spots" on the T line, significantly amplifying the SERS signals. Using this 
      strategy, the limits of detections were low to aM level, and the selectivity, 
      reproducibility, and uniformity of the proposed SERS-LFA biosensor were 
      satisfactory. Finally, this rapid analysis strategy was successfully applied to 
      quantitatively detect the target miRNAs in clinical serum obtained from healthy 
      subjects and patients with LSCC at different stages. The results were consistent 
      with the quantitative real-time PCR (qRT-PCR). Thus, the CHA-assisted SERS-LFA 
      biosensor would become a promising alternative tool for miRNAs detection, which 
      showed a tremendous clinical application prospect in diagnosing LSCC.
CI  - Copyright (c) 2022 Li, Niu, Ge, Cao and Sun.
FAU - Li, Guang
AU  - Li G
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, The Affiliated Hospital 
      of Yangzhou University, Yangzhou University, Yangzhou, China.
FAU - Niu, Ping
AU  - Niu P
AD  - Departments of Otolaryngology, The Affiliated Hospital of Shandong First Medical 
      University, Qingzhou People's Hospital, Qingzhou, China.
FAU - Ge, Shengjie
AU  - Ge S
AD  - Institute of Translational Medicine, Medical College, Yangzhou University, 
      Yangzhou, China.
FAU - Cao, Dawei
AU  - Cao D
AD  - College of Mathematics and Computer Science, Zhejiang Normal University, Jinhua, 
      China.
FAU - Sun, Aidong
AU  - Sun A
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, The Affiliated Hospital 
      of Yangzhou University, Yangzhou University, Yangzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20220211
PL  - Switzerland
TA  - Front Mol Biosci
JT  - Frontiers in molecular biosciences
JID - 101653173
PMC - PMC8878268
OTO - NOTNLM
OT  - Pd-Au core-shell nanorods
OT  - catalytic hairpin assembly
OT  - laryngeal squamous cell carcinoma
OT  - lateral flow assay
OT  - miRNA
OT  - surface-enhanced Raman scattering
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/03/01 06:00
MHDA- 2022/03/01 06:01
PMCR- 2021/01/01
CRDT- 2022/02/28 05:40
PHST- 2021/11/12 00:00 [received]
PHST- 2021/12/20 00:00 [accepted]
PHST- 2022/02/28 05:40 [entrez]
PHST- 2022/03/01 06:00 [pubmed]
PHST- 2022/03/01 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 813007 [pii]
AID - 10.3389/fmolb.2021.813007 [doi]
PST - epublish
SO  - Front Mol Biosci. 2022 Feb 11;8:813007. doi: 10.3389/fmolb.2021.813007. 
      eCollection 2021.