PMID- 35224160
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220301
IS  - 2352-3042 (Electronic)
IS  - 2352-4820 (Print)
IS  - 2352-3042 (Linking)
VI  - 9
IP  - 2
DP  - 2022 Mar
TI  - Long non-coding RNA HCAR promotes endochondral bone repair by upregulating VEGF 
      and MMP13 in hypertrophic chondrocyte through sponging miR-15b-5p.
PG  - 456-465
LID - 10.1016/j.gendis.2020.07.013 [doi]
AB  - Endochondral bone formation is an important route for bone repair. Although 
      emerging evidence has revealed the functions of long non-coding RNAs (lncRNAs) in 
      bone and cartilage development, the effect of lncRNAs in endochondral bone repair 
      is still largely unknown. Here, we identified a lncRNA, named Hypertrophic 
      Chondrocyte Angiogenesis-related lncRNA (HCAR), and proved it to promote the 
      endochondral bone repair by upregulating the expression of matrix 
      metallopeptidase 13 (Mmp13) and vascular endothelial growth factor alpha (Vegfa) in 
      hypertrophic chondrocytes. Lnc-HCAR knockdown in hypertrophic chondrocytes 
      restrained the cartilage matrix remodeling and decrease the CD31(hi)Emcn(hi) 
      vessels number in a bone repair model. Mechanistically, we proved that lnc-HCAR 
      was mainly enriched in the cytoplasm using fluorescence in situ hybridization 
      (FISH) assay, and it acted as a molecular sponge for miR-15b-5p. Further, in 
      hypertrophic chondrocytes, lnc-HCAR competitively bound to miR-15b-5p to increase 
      Vegfa and Mmp13 expression. Our results proved that lncRNA is deeply involved in 
      endochondral bone repair, which will provide a new theoretical basis for future 
      strategies for promoting fracture healing.
CI  - (c) 2021 Chongqing Medical University. Production and hosting by Elsevier B.V.
FAU - Bai, Yun
AU  - Bai Y
AD  - Department of Biomedical Materials Science, School of Biomedical Engineering, 
      Third Military Medical University, Chongqing 400038, PR China.
FAU - Gong, Xiaoshan
AU  - Gong X
AD  - Department of Biomedical Materials Science, School of Biomedical Engineering, 
      Third Military Medical University, Chongqing 400038, PR China.
FAU - Dong, Rui
AU  - Dong R
AD  - Department of Biomedical Materials Science, School of Biomedical Engineering, 
      Third Military Medical University, Chongqing 400038, PR China.
FAU - Cao, Zhen
AU  - Cao Z
AD  - Department of Biomedical Materials Science, School of Biomedical Engineering, 
      Third Military Medical University, Chongqing 400038, PR China.
FAU - Dou, Ce
AU  - Dou C
AD  - Department of Biomedical Materials Science, School of Biomedical Engineering, 
      Third Military Medical University, Chongqing 400038, PR China.
FAU - Liu, Chuan
AU  - Liu C
AD  - Department of Biomedical Materials Science, School of Biomedical Engineering, 
      Third Military Medical University, Chongqing 400038, PR China.
AD  - Institute of Trauma Orthopedics, Army General Hospital of PLA, Beijing 100700, PR 
      China.
FAU - Li, Jianmei
AU  - Li J
AD  - Department of Biomedical Materials Science, School of Biomedical Engineering, 
      Third Military Medical University, Chongqing 400038, PR China.
FAU - Kang, Fei
AU  - Kang F
AD  - Department of Biomedical Materials Science, School of Biomedical Engineering, 
      Third Military Medical University, Chongqing 400038, PR China.
FAU - Dai, Jingjin
AU  - Dai J
AD  - Department of Biomedical Materials Science, School of Biomedical Engineering, 
      Third Military Medical University, Chongqing 400038, PR China.
FAU - Zhao, Chunrong
AU  - Zhao C
AD  - Department of Biomedical Materials Science, School of Biomedical Engineering, 
      Third Military Medical University, Chongqing 400038, PR China.
FAU - Tian, Zhansong
AU  - Tian Z
AD  - Department of Biomedical Materials Science, School of Biomedical Engineering, 
      Third Military Medical University, Chongqing 400038, PR China.
FAU - Tan, Jiulin
AU  - Tan J
AD  - Department of Orthopedics, Southwest Hospital, Third Military Medical University, 
      Chongqing 400038, PR China.
FAU - Dai, Qijie
AU  - Dai Q
AD  - Department of Orthopedics, Southwest Hospital, Third Military Medical University, 
      Chongqing 400038, PR China.
FAU - Dong, Shiwu
AU  - Dong S
AD  - Department of Biomedical Materials Science, School of Biomedical Engineering, 
      Third Military Medical University, Chongqing 400038, PR China.
AD  - Department of Orthopedics, Southwest Hospital, Third Military Medical University, 
      Chongqing 400038, PR China.
AD  - State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical 
      University, Chongqing 400038, PR China.
LA  - eng
PT  - Journal Article
DEP - 20200810
PL  - Netherlands
TA  - Genes Dis
JT  - Genes & diseases
JID - 101635967
PMC - PMC8843884
OTO - NOTNLM
OT  - BMSCs, bone marrow mesenchymal stem cells
OT  - Bone marrow mesenchymal stem cells
OT  - Bone repair
OT  - Chondrocyte
OT  - Enchondral bone repair
OT  - HCAR, hypertrophic chondrocyte angiogenesis-related lncRNA
OT  - Hoxa2, homeobox A2
OT  - Long non coding RNA
OT  - Mef2c, myocyte enhancer factor 2C
OT  - Mmp13, matrix metallopeptidase 13
OT  - OA, osteoarthritis
OT  - Osx, osterix
OT  - Runx2, runt-related transcription factor 2
OT  - Vegfa, vascular endothelial growth factor alpha
OT  - lncRNA, long no-coding RNA
EDAT- 2020/08/10 00:00
MHDA- 2020/08/10 00:01
PMCR- 2020/08/10
CRDT- 2022/02/28 05:40
PHST- 2020/04/16 00:00 [received]
PHST- 2020/06/08 00:00 [revised]
PHST- 2020/07/30 00:00 [accepted]
PHST- 2022/02/28 05:40 [entrez]
PHST- 2020/08/10 00:00 [pubmed]
PHST- 2020/08/10 00:01 [medline]
PHST- 2020/08/10 00:00 [pmc-release]
AID - S2352-3042(20)30096-9 [pii]
AID - 10.1016/j.gendis.2020.07.013 [doi]
PST - epublish
SO  - Genes Dis. 2020 Aug 10;9(2):456-465. doi: 10.1016/j.gendis.2020.07.013. 
      eCollection 2022 Mar.