PMID- 35225219 OWN - NLM STAT- MEDLINE DCOM- 20220301 LR - 20220405 IS - 0392-856X (Print) IS - 0392-856X (Linking) VI - 40 IP - 2 DP - 2022 Feb TI - MyomiRs in cultured muscle cells from patients with idiopathic inflammatory myopathy are modulated by disease but not by 6-month exercise training. PG - 346-357 LID - 10.55563/clinexprheumatol/69z7za [doi] AB - OBJECTIVES: Idiopathic inflammatory myopathies/IIM are associated with changes in muscle-specific microRNA/miR. Exercise improves muscle function and metabolism in parallel with changes in miR expression. We investigated the effects of disease and exercise on miRs in differentiated muscle cells/myotubes from IIM patients and controls. METHODS: Samples of m. vastus lateralis were obtained by needle biopsy from IIM patients before/after 6-month training and from matched sedentary healthy controls. Muscle cell cultures were established and exposed to saturated fatty acid during differentiation. MiR-133a,-133b,-206,-1 and their target genes (qPCR), fat oxidation (FOx), lipids (chromatography) and mitochondrial oxidative phosphorylation (OxPHOS) complexes (immunoblotting) were measured. Interrelations between in vitro miRs and metabolism of myotubes as well as clinical parameters and disease activity/MITAX were explored. RESULTS: Levels of miRs were higher in myotubes derived from IIM patients compared to healthy controls (up to 3.5-fold, p<0.05). Neither 6-month training (IIM patients) nor in vitro palmitate treatment modulated myomiRs in myotubes. However, miR-133a,-133b, and miR-1 correlated negatively with FOx (p<0.01), triacylglycerols (p<0.05) and OxPHOS complex-V (p<0.05) and positively with OxPHOS complex-I (p<0.05) in myotubes. MiR-133a and miR-133b in myotubes were related to disease activity and fasting glycaemia in vivo (both p<0.05). CONCLUSIONS: Upregulation of microRNAs involved in myogenesis and regeneration in muscle cells derived from IIM patients indicates activation of compensatory epigenetic mechanisms, potentially aimed to counteract disease progression. Relationships of microRNAs with in vitro metabolic profile of muscle cells as well as with clinical parameters support the role of muscle-specific microRNAs in modulating muscle metabolism and clinical state of patients. FAU - Alchus Laiferova, Nikoleta AU - Alchus Laiferova N AD - Institute of Experimental Endocrinology, Biomedical Research Centre, Slovak Academy of Sciences, Bratislava, and Institute of Pathophysiology, Faculty of Medicine, Comenius University, Bratislava, Slovakia. FAU - Nemec, Michal AU - Nemec M AD - Institute of Experimental Endocrinology, Biomedical Research Centre, Slovak Academy of Sciences, Bratislava, Slovakia. FAU - Vernerova, Lucia AU - Vernerova L AD - Institute of Rheumatology, Prague, Czech Republic. FAU - Balazova, Maria AU - Balazova M AD - Centre of Biosciences, Slovak Academy of Sciences, Bratislava, Slovakia. FAU - Vokurkova, Martina AU - Vokurkova M AD - Institute of Rheumatology, Prague, Czech Republic. FAU - Oreska, Sabina AU - Oreska S AD - Institute of Rheumatology, Prague, and Department of Rheumatology, First Faculty of Medicine, Charles University, Prague, Czech Republic. FAU - Klein, Martin AU - Klein M AD - Institute of Rheumatology, Prague, and Department of Rheumatology, First Faculty of Medicine, Charles University, Prague, Czech Republic. FAU - Spiritovic, Maja AU - Spiritovic M AD - Institute of Rheumatology, Prague, and Department of Physiotherapy, Faculty of Physical Education and Sport, Charles University, Prague, Czech Republic. FAU - Tomcik, Michal AU - Tomcik M AD - Department of Rheumatology, First Faculty of Medicine, Charles University, Prague, Czech Republic. FAU - Vencovsky, Jiri AU - Vencovsky J AD - Institute of Rheumatology, Prague, and Department of Rheumatology, First Faculty of Medicine, Charles University, Prague, Czech Republic. FAU - Ukropec, Jozef AU - Ukropec J AD - Institute of Experimental Endocrinology, Biomedical Research Centre, Slovak Academy of Sciences, Bratislava, Slovakia. FAU - Ukropcova, Barbara AU - Ukropcova B AD - Institute of Experimental Endocrinology, Biomedical Research Centre, Slovak Academy of Sciences, Bratislava, and Institute of Pathophysiology, Faculty of Medicine, Comenius University, Bratislava, Slovakia. barbara.ukropcova@savba.sk. LA - eng PT - Journal Article DEP - 20220225 PL - Italy TA - Clin Exp Rheumatol JT - Clinical and experimental rheumatology JID - 8308521 RN - 0 (MicroRNAs) SB - IM MH - Cells, Cultured MH - Exercise/physiology MH - Humans MH - *MicroRNAs/genetics/metabolism MH - Muscle Fibers, Skeletal/metabolism/pathology MH - Muscle, Skeletal/physiology MH - *Myositis/pathology EDAT- 2022/03/01 06:00 MHDA- 2022/03/03 06:00 CRDT- 2022/02/28 08:56 PHST- 2021/09/20 00:00 [received] PHST- 2022/01/17 00:00 [accepted] PHST- 2022/02/28 08:56 [entrez] PHST- 2022/03/01 06:00 [pubmed] PHST- 2022/03/03 06:00 [medline] AID - 17925 [pii] AID - 10.55563/clinexprheumatol/69z7za [doi] PST - ppublish SO - Clin Exp Rheumatol. 2022 Feb;40(2):346-357. doi: 10.55563/clinexprheumatol/69z7za. Epub 2022 Feb 25.