PMID- 35225678
OWN - NLM
STAT- MEDLINE
DCOM- 20220316
LR  - 20220316
IS  - 1945-0257 (Electronic)
IS  - 1945-0257 (Linking)
VI  - 26
IP  - 2
DP  - 2022 Feb
TI  - Bacterial Identification and Visualization of Bacterial Biofilms Adjacent to 
      Fracture Sites After Internal Fixation.
PG  - 70-80
LID - 10.1089/gtmb.2019.0225 [doi]
AB  - Objectives: The primary aims of this study were to determine if any correlation 
      exists in cases of fracture fixation among: (1) bacterial profiles recovered from 
      the instrumentation and adjacent tissues; (2) the type of orthopedic injury; and 
      (3) the clinical outcome-union versus nonunion. A secondary goal was to compare 
      culture and molecular diagnostics for identifying the bacterial species present 
      following fracture fixation. Design: Single-institution, prospective case-control 
      cohort study. Setting: Single level 1 trauma center. Patients: Forty-nine bony 
      nonunion cases undergoing revision internal fixation and 45 healed fracture 
      controls undergoing removal of hardware. Intervention: Bacterial infection was 
      detected by standard microbial culture methods and by a pan-eubacterial domain, 
      molecular diagnostic (MDx) assay. Confirmation of culture and MDx results was 
      achieved with bacterial ribosomal 16S rRNA fluorescence in situ hybridization 
      (FISH) to visualize bacterial biofilms. Main Outcome Measurements: MDx and 
      microbial culture methods results were the primary study outcomes. Results: 
      Ninety-four percent of the nonunion cohort and 93% of the union cohort had 
      bacteria detected by the MDx. Seventy-eight percent of the nonunion cases and 69% 
      of the controls were culture negative, but MDx positive. Although no significant 
      differences in bacterial composition were observed between the cases and 
      controls, differences were observed when cases were divided by comorbidities. 
      Conclusion: The MDx is more sensitive than microbial culture in detecting 
      bacterial presence. The lack of significantly different findings with regard to 
      bacterial profile identified between the cases and controls suggests that host 
      factors and environmental conditions are largely responsible for determining if 
      bony union will occur. Level of Evidence: Diagnostic Level III. See Instructions 
      for Authors for a complete description of levels of evidence.
FAU - Palmer, Michael P
AU  - Palmer MP
AD  - The Christ Hospital Network, Cincinnati, Ohio, USA.
FAU - Altman, Daniel T
AU  - Altman DT
AD  - Department of Orthopedics, Allegheny General Hospital, Pittsburgh, Pennsylvania, 
      USA.
FAU - Altman, Gregory T
AU  - Altman GT
AD  - Department of Orthopedics, Allegheny General Hospital, Pittsburgh, Pennsylvania, 
      USA.
FAU - Sewecke, Jeffrey J
AU  - Sewecke JJ
AD  - Department of Orthopedics, Allegheny General Hospital, Pittsburgh, Pennsylvania, 
      USA.
FAU - Saltarski, Courtney
AU  - Saltarski C
AD  - Harold C. Simmons Comprehensive Cancer Center, UT Southwestern Medical Center, 
      Dallas, Texas, USA.
FAU - Nistico, Laura
AU  - Nistico L
AD  - Center of Excellence in Biofilm Research Genomic Sciences, Allegheny Singer 
      Research Institute, Allegheny General Hospital, Pittsburgh, Pennsylvania, USA.
FAU - Melton-Kreft, Rachael
AU  - Melton-Kreft R
AD  - Center of Excellence in Biofilm Research Genomic Sciences, Allegheny Singer 
      Research Institute, Allegheny General Hospital, Pittsburgh, Pennsylvania, USA.
FAU - Hu, Fen Z
AU  - Hu FZ
AD  - Center for Biofilms and Surgical Infections, Center for Genomic Sciences, and 
      Center for Advanced Microbial Processing, Institute of Molecular Medicine and 
      Infectious Disease, Philadelphia, Pennsylvania, USA.
AD  - Departments of Microbiology and Immunology, and Otolaryngology Head and Neck 
      Surgery, Drexel University College of Medicine, Philadelphia, Pennsylvania, USA.
FAU - Ehrlich, Garth D
AU  - Ehrlich GD
AD  - Center for Biofilms and Surgical Infections, Center for Genomic Sciences, and 
      Center for Advanced Microbial Processing, Institute of Molecular Medicine and 
      Infectious Disease, Philadelphia, Pennsylvania, USA.
AD  - Departments of Microbiology and Immunology, and Otolaryngology Head and Neck 
      Surgery, Drexel University College of Medicine, Philadelphia, Pennsylvania, USA.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Genet Test Mol Biomarkers
JT  - Genetic testing and molecular biomarkers
JID - 101494210
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Bacteria/genetics
MH  - Biofilms
MH  - Case-Control Studies
MH  - *Fractures, Ununited/diagnosis/microbiology/surgery
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - RNA, Ribosomal, 16S/genetics
MH  - Retrospective Studies
MH  - Treatment Outcome
OTO - NOTNLM
OT  - FISH
OT  - bacteria
OT  - bony nonunion
OT  - infected implant
OT  - molecular diagnostics
OT  - tissue culture
EDAT- 2022/03/01 06:00
MHDA- 2022/03/17 06:00
CRDT- 2022/02/28 12:14
PHST- 2022/02/28 12:14 [entrez]
PHST- 2022/03/01 06:00 [pubmed]
PHST- 2022/03/17 06:00 [medline]
AID - 10.1089/gtmb.2019.0225 [doi]
PST - ppublish
SO  - Genet Test Mol Biomarkers. 2022 Feb;26(2):70-80. doi: 10.1089/gtmb.2019.0225.