PMID- 35226830
OWN - NLM
STAT- MEDLINE
DCOM- 20220405
LR  - 20220405
IS  - 2165-5987 (Electronic)
IS  - 2165-5979 (Print)
IS  - 2165-5979 (Linking)
VI  - 13
IP  - 3
DP  - 2022 Mar
TI  - MicroRNA-200b-3p restrains gastric cancer cell proliferation, migration, and 
      invasion via C-X-C motif chemokine ligand 12/CXC chemokine receptor 7 axis.
PG  - 6509-6520
LID - 10.1080/21655979.2022.2034585 [doi]
AB  - This study was conducted to investigate the impact of microRNA (miR)-200b-3p on 
      viability, migration, and invasion of gastric cancer (GC) cells and its 
      mechanism. Quantitative real-time PCR (qRT-PCR) was conducted to measure 
      miR-200b-3p expression in GC tissues and cells; besides, the relationship between 
      miR-200b-3p expression and overall survival time (OS) was analyzed with OncomiR 
      database; cell counting kit-8 (CCK-8), colony formation assay, flow cytometry, 
      scratch healing assay, and Transwell assay were performed to detect the 
      proliferation, cell cycle progression, migration, and invasion of GC cells; a 
      lung metastasis model in nude mice was used to examine the effect of miR-200b-3p 
      on the metastasis of GC cells in vivo; the interplay between miR-200b-3p and 
      C-X-C motif chemokine ligand 12 (CXCL12) mRNA 3' UTR was predicted by 
      bioinformatics and verified with a dual-luciferase reporter gene assay; besides, 
      the expression of CXCL12 and CXC chemokine receptor 7 (CXCR7) was probed by 
      Western blot. It was found that miR-200b-3p expression was down-regulated in GC 
      tissues, which was remarkably associated with the lymph node metastasis and 
      decrease of differentiation of GC; transfection with miR-200b-3p mimics 
      restrained the growth, migration, and invasion of GC cells in vitro, induced cell 
      cycle arrest, and inhibited CXCL12 and CXCR7 expression levels; transfection of 
      miR-200b-3p inhibitors worked oppositely in vitro and promoted lung metastasis in 
      vivo. CXCL12 was confirmed as the downstream target of miR-200b-3p and was 
      negatively modulated by miR-200b-3p. In conclusion, miR-200b-3p inhibited GC 
      progression via regulating CXCL12/CXCR7 axis.
FAU - Li, Dinuo
AU  - Li D
AUID- ORCID: 0000-0001-7317-5045
AD  - Department of General Gastropathy, The First Affiliated Hospital of Jinzhou 
      Medical University, Jinzhou, China.
FAU - Li, Qiang
AU  - Li Q
AD  - Department of Gastrosurgery, The First Affiliated Hospital of Jinzhou Medical 
      University, Jinzhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Bioengineered
JT  - Bioengineered
JID - 101581063
RN  - 0 (ACKR3 protein, human)
RN  - 0 (CXCL12 protein, human)
RN  - 0 (Chemokine CXCL12)
RN  - 0 (MIRN200 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Receptors, CXCR)
SB  - IM
MH  - Animals
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - Chemokine CXCL12/*genetics
MH  - Humans
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - MicroRNAs/*genetics
MH  - Middle Aged
MH  - Receptors, CXCR/*genetics
MH  - Signal Transduction/genetics
MH  - *Stomach Neoplasms/genetics/metabolism/pathology
PMC - PMC8974025
OTO - NOTNLM
OT  - CXCL12/CXCR7
OT  - GC
OT  - miR-200b-3p
OT  - migration and invasion
OT  - proliferation
COIS- No potential conflict of interest was reported by the author(s).
EDAT- 2022/03/01 06:00
MHDA- 2022/04/06 06:00
PMCR- 2022/02/28
CRDT- 2022/02/28 20:03
PHST- 2022/02/28 20:03 [entrez]
PHST- 2022/03/01 06:00 [pubmed]
PHST- 2022/04/06 06:00 [medline]
PHST- 2022/02/28 00:00 [pmc-release]
AID - 2034585 [pii]
AID - 10.1080/21655979.2022.2034585 [doi]
PST - ppublish
SO  - Bioengineered. 2022 Mar;13(3):6509-6520. doi: 10.1080/21655979.2022.2034585.