PMID- 35228497
OWN - NLM
STAT- MEDLINE
DCOM- 20220302
LR  - 20220302
IS  - 1881-7742 (Electronic)
IS  - 0301-4800 (Linking)
VI  - 68
IP  - 1
DP  - 2022
TI  - Vitamin D Activates Various Gene Expressions, Including Lipid Metabolism, in 
      C2C12 Cells.
PG  - 65-72
LID - 10.3177/jnsv.68.65 [doi]
AB  - Vitamin D is a fat-soluble molecule, well known for its role in regulating 
      calcium homeostasis in bone. It has become increasingly clear that it also has 
      important effects in many other organs, including the skeletal muscle. In order 
      to gain insight into the role of vitamin D in the skeletal muscle, we performed 
      microarray analysis using C2C12 myoblasts treated with 1,25-dihydroxyvitamin D 
      (1,25(OH)(2)D), active form of vitamin D. We found multiple genes upregulated by 
      1,25(OH)(2)D. Some of them, i.e., vitamin D receptor (Vdr), diacylglycerol 
      O-acyltransferase (Dgat1 and Dgat2, the rate limiting steps of triacylglycerol 
      acylation), and vascular endothelial growth factor A (Vegfa), were previously 
      reported to be upregulated by 1,25(OH)(2)D in C2C12 cells. RT-qPCR analysis 
      confirmed increased mRNA levels of Rarres2, Dio2, Tgm2, Lpl, Mdfi, Igfbp3, Dgat1, 
      Crabp2, Gadd45a, Vagfa, Dgat2, C3, Ldhb, Cebpa, Igfbp5, Mrc2, Vdr. Thus, many 
      genes, including lipid metabolism genes as well as genes related to muscle 
      functions, appear to be upregulated by 1,25(OH)(2)D in muscle cells.
FAU - Mizutani, Sako
AU  - Mizutani S
AD  - Laboratory of Molecular Nutrition, Graduate School of Life and Environmental 
      Sciences, Kyoto Prefectural University.
FAU - Oyabu, Mamoru
AU  - Oyabu M
AD  - Laboratory of Molecular Nutrition, Graduate School of Life and Environmental 
      Sciences, Kyoto Prefectural University.
FAU - Yamamoto, Arisa
AU  - Yamamoto A
AD  - Laboratory of Molecular Nutrition, Graduate School of Life and Environmental 
      Sciences, Kyoto Prefectural University.
FAU - Uchitomi, Ran
AU  - Uchitomi R
AD  - Laboratory of Molecular Nutrition, Graduate School of Life and Environmental 
      Sciences, Kyoto Prefectural University.
FAU - Sugimoto, Takumi
AU  - Sugimoto T
AD  - Laboratory of Molecular Nutrition, Graduate School of Life and Environmental 
      Sciences, Kyoto Prefectural University.
FAU - Kamei, Yasutomi
AU  - Kamei Y
AD  - Laboratory of Molecular Nutrition, Graduate School of Life and Environmental 
      Sciences, Kyoto Prefectural University.
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - J Nutr Sci Vitaminol (Tokyo)
JT  - Journal of nutritional science and vitaminology
JID - 0402640
RN  - 0 (Receptors, Calcitriol)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (Vitamins)
RN  - 1406-16-2 (Vitamin D)
SB  - IM
MH  - Gene Expression
MH  - Lipid Metabolism/genetics
MH  - Receptors, Calcitriol/genetics/metabolism
MH  - *Vascular Endothelial Growth Factor A/metabolism
MH  - *Vitamin D/metabolism/pharmacology
MH  - Vitamins
OTO - NOTNLM
OT  - C2C12 myoblasts
OT  - lipid metabolism
OT  - nuclear receptor
OT  - skeletal muscle
OT  - vitamin D
EDAT- 2022/03/02 06:00
MHDA- 2022/03/03 06:00
CRDT- 2022/03/01 05:55
PHST- 2022/03/01 05:55 [entrez]
PHST- 2022/03/02 06:00 [pubmed]
PHST- 2022/03/03 06:00 [medline]
AID - 10.3177/jnsv.68.65 [doi]
PST - ppublish
SO  - J Nutr Sci Vitaminol (Tokyo). 2022;68(1):65-72. doi: 10.3177/jnsv.68.65.