PMID- 35230542
OWN - NLM
STAT- MEDLINE
DCOM- 20220308
LR  - 20220716
IS  - 1420-9071 (Electronic)
IS  - 1420-682X (Print)
IS  - 1420-682X (Linking)
VI  - 79
IP  - 3
DP  - 2022 Mar 1
TI  - PP2A-Cdc55 phosphatase regulates actomyosin ring contraction and septum formation 
      during cytokinesis.
PG  - 165
LID - 10.1007/s00018-022-04209-1 [doi]
LID - 165
AB  - Eukaryotic cells divide and separate all their components after chromosome 
      segregation by a process called cytokinesis to complete cell division. 
      Cytokinesis is highly regulated by the recruitment of the components to the 
      division site and through post-translational modifications such as 
      phosphorylations. The budding yeast mitotic kinases Cdc28-Clb2, Cdc5, and 
      Dbf2-Mob1 phosphorylate several cytokinetic proteins contributing to the 
      regulation of cytokinesis. The PP2A-Cdc55 phosphatase regulates mitosis 
      counteracting Cdk1- and Cdc5-dependent phosphorylation. This prompted us to 
      propose that PP2A-Cdc55 could also be counteracting the mitotic kinases during 
      cytokinesis. Here we show that in the absence of Cdc55, AMR contraction and the 
      primary septum formation occur asymmetrically to one side of the bud neck 
      supporting a role for PP2A-Cdc55 in cytokinesis regulation. In addition, by in 
      vivo and in vitro assays, we show that PP2A-Cdc55 dephosphorylates the chitin 
      synthase II (Chs2 in budding yeast) a component of the Ingression Progression 
      Complexes (IPCs) involved in cytokinesis. Interestingly, the non-phosphorylable 
      version of Chs2 rescues the asymmetric AMR contraction and the defective septa 
      formation observed in cdc55∆ mutant cells. Therefore, timely dephosphorylation of 
      the Chs2 by PP2A-Cdc55 is crucial for proper actomyosin ring contraction. These 
      findings reveal a new mechanism of cytokinesis regulation by the PP2A-Cdc55 
      phosphatase and extend our knowledge of the involvement of multiple phosphatases 
      during cytokinesis.
CI  - (c) 2022. The Author(s).
FAU - Moyano-Rodriguez, Yolanda
AU  - Moyano-Rodriguez Y
AD  - Cell Cycle Group, Institut d'Investigacio Biomedica de Bellvitge (IDIBELL), Av. 
      Gran Via de L'Hospitalet 199-203, L'Hospitalet de Llobregat, Barcelona, Spain.
FAU - Vaquero, David
AU  - Vaquero D
AD  - Cell Cycle Group, Institut d'Investigacio Biomedica de Bellvitge (IDIBELL), Av. 
      Gran Via de L'Hospitalet 199-203, L'Hospitalet de Llobregat, Barcelona, Spain.
AD  - Instituto de Biomedicina de Valencia (IBV-CSIC), C/ Jaume Roig 11, Valencia, 
      Spain.
FAU - Vilalta-Castany, Odena
AU  - Vilalta-Castany O
AD  - Cell Cycle Group, Institut d'Investigacio Biomedica de Bellvitge (IDIBELL), Av. 
      Gran Via de L'Hospitalet 199-203, L'Hospitalet de Llobregat, Barcelona, Spain.
FAU - Foltman, Magdalena
AU  - Foltman M
AD  - Instituto de Biomedicina y Biotecnologia de Cantabria, Universidad de Cantabria, 
      CSIC, Santander, Spain.
AD  - Departamento de Biologia Molecular, Facultad de Medicina, Universidad de 
      Cantabria, Santander, Spain.
FAU - Sanchez-Diaz, Alberto
AU  - Sanchez-Diaz A
AD  - Instituto de Biomedicina y Biotecnologia de Cantabria, Universidad de Cantabria, 
      CSIC, Santander, Spain.
AD  - Departamento de Biologia Molecular, Facultad de Medicina, Universidad de 
      Cantabria, Santander, Spain.
FAU - Queralt, Ethel
AU  - Queralt E
AUID- ORCID: 0000-0003-0045-0039
AD  - Cell Cycle Group, Institut d'Investigacio Biomedica de Bellvitge (IDIBELL), Av. 
      Gran Via de L'Hospitalet 199-203, L'Hospitalet de Llobregat, Barcelona, Spain. 
      equeralt@ibv.csic.es.
AD  - Instituto de Biomedicina de Valencia (IBV-CSIC), C/ Jaume Roig 11, Valencia, 
      Spain. equeralt@ibv.csic.es.
LA  - eng
GR  - PID2019-109027GB-I00/Ministerio de Ciencia, Innovacion y Universidades/
GR  - PID2019-106745GB-I00/Ministerio de Ciencia, Innovacion y Universidades/
GR  - BFU2016-77975-R/Ministerio de Economia, Industria y Competitividad, Gobierno de 
      Espana/
PT  - Journal Article
DEP - 20220301
PL  - Switzerland
TA  - Cell Mol Life Sci
JT  - Cellular and molecular life sciences : CMLS
JID - 9705402
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 9013-26-7 (Actomyosin)
RN  - EC 2.4.1.16 (Chitin Synthase)
RN  - EC 2.4.1.16 (chitin synthase 2)
SB  - IM
MH  - Actomyosin/*metabolism
MH  - Chitin Synthase/metabolism
MH  - Chromosome Segregation/physiology
MH  - Cytokinesis/*physiology
MH  - Phosphorylation/physiology
MH  - Saccharomyces cerevisiae Proteins/metabolism
MH  - Saccharomycetales/metabolism
PMC - PMC8888506
OTO - NOTNLM
OT  - AMR contraction
OT  - Cell Cycle
OT  - Cyk3
OT  - Cytokinesis
OT  - Hof1
OT  - Ingression progression complexes (IPCs)
OT  - Myo1
OT  - PP2A-Cdc55
COIS- The authors declare no competing interests.
EDAT- 2022/03/02 06:00
MHDA- 2022/03/09 06:00
PMCR- 2022/03/01
CRDT- 2022/03/01 12:17
PHST- 2021/09/01 00:00 [received]
PHST- 2022/02/13 00:00 [accepted]
PHST- 2022/02/11 00:00 [revised]
PHST- 2022/03/01 12:17 [entrez]
PHST- 2022/03/02 06:00 [pubmed]
PHST- 2022/03/09 06:00 [medline]
PHST- 2022/03/01 00:00 [pmc-release]
AID - 10.1007/s00018-022-04209-1 [pii]
AID - 4209 [pii]
AID - 10.1007/s00018-022-04209-1 [doi]
PST - epublish
SO  - Cell Mol Life Sci. 2022 Mar 1;79(3):165. doi: 10.1007/s00018-022-04209-1.