PMID- 35231655
OWN - NLM
STAT- MEDLINE
DCOM- 20220411
LR  - 20220411
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 601
DP  - 2022 Apr 23
TI  - Transcriptome analysis of long non-coding RNAs reveals NR_015556 lncRNA is a 
      novel regulator for adipocyte differentiation.
PG  - 79-85
LID - S0006-291X(22)00259-5 [pii]
LID - 10.1016/j.bbrc.2022.02.069 [doi]
AB  - Long non-coding RNAs (lncRNAs) have gained extensive attentions due to their 
      significant roles in diverse biological process. However, the potential functions 
      of lncRNAs participation in adipocyte differentiation have not been fully 
      explored. In the present study, we globally profiled lncRNA expression using 
      lncRNA microarray and identified 1745 lncRNA probes with differential expression 
      on day 0 and day 4 post-induction in both C3H10T1/2 mesenchymal stem cells and 
      3T3-L1 preadipocytes. Furthermore, we showed that stable shRNA knockdown (KD) of 
      NR_015556, a novel lncRNA that was significantly down-regulated in adipocyte 
      differentiation, promoted adipocyte differentiation by increasing the number of 
      lipid droplets and adipocyte markers such as Fabp4, Adipsin and Fasn. 
      Mechanistically, NR_015556 KD attenuated the expression of Wnt signaling 
      components Wnt10b and non-phospho (active) beta-catenin, and elevated adipocyte 
      master factors Ppar-gamma and C/EBPalpha levels. Conversely, pharmacological activation 
      of Wnt10b-beta-catenin signaling by LiCl suppressed NR_015556 KD-induced enhancement 
      of adipocyte differentiation and Ppar-gamma and C/EBPalpha expression levels. Taken 
      together, these results indicate that down-regulation of NR_015556 promotes 
      adipocyte differentiation through inhibiting Wnt10b-beta-catenin signaling pathway 
      and then elevating Ppar-gamma and C/EBPalpha triggered transcriptional cascades.
CI  - Copyright (c) 2022 Elsevier Inc. All rights reserved.
FAU - Zuo, Changqing
AU  - Zuo C
AD  - Guangdong Provincial Key Laboratory of Research and Development of Natural Drugs, 
      And School of Pharmacy, Guangdong Medical University, Dongguan, 523808, PR China; 
      Key Laboratory of Traditional Chinese Medicine and New Pharmacy Development, 
      Guangdong Medical University, Dongguan, 523808, PR China.
FAU - Pan, Yaqiong
AU  - Pan Y
AD  - Guangdong Provincial Key Laboratory of Research and Development of Natural Drugs, 
      And School of Pharmacy, Guangdong Medical University, Dongguan, 523808, PR China; 
      Department of Pharmacy, Dongguan Liaobu Hospital, Guangdong Medical University, 
      Dongguan, 523400, PR China.
FAU - Leng, Dan
AU  - Leng D
AD  - Guangdong Provincial Key Laboratory of Research and Development of Natural Drugs, 
      And School of Pharmacy, Guangdong Medical University, Dongguan, 523808, PR China.
FAU - Chen, Xiuju
AU  - Chen X
AD  - Guangdong Provincial Key Laboratory of Research and Development of Natural Drugs, 
      And School of Pharmacy, Guangdong Medical University, Dongguan, 523808, PR China.
FAU - Dong, Fanghongniu
AU  - Dong F
AD  - Guangdong Provincial Key Laboratory of Research and Development of Natural Drugs, 
      And School of Pharmacy, Guangdong Medical University, Dongguan, 523808, PR China.
FAU - Lin, Zhanying
AU  - Lin Z
AD  - Guangdong Provincial Key Laboratory of Research and Development of Natural Drugs, 
      And School of Pharmacy, Guangdong Medical University, Dongguan, 523808, PR China.
FAU - Dai, Zhong
AU  - Dai Z
AD  - Guangdong Provincial Key Laboratory of Research and Development of Natural Drugs, 
      And School of Pharmacy, Guangdong Medical University, Dongguan, 523808, PR China; 
      Key Laboratory of Traditional Chinese Medicine and New Pharmacy Development, 
      Guangdong Medical University, Dongguan, 523808, PR China. Electronic address: 
      daizhong_gdmu@163.com.
FAU - Wang, Zonggui
AU  - Wang Z
AD  - Department of Biochemistry and Molecular Biology, Guangdong Provincial Key 
      Laboratory of Medical Molecular Diagnostics, Guangdong Medical University, 
      Dongguan, 523808, PR China. Electronic address: wangzonggui@gdmu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220222
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (CCAAT-Enhancer-Binding Protein-alpha)
RN  - 0 (PPAR gamma)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (beta Catenin)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipocytes/metabolism
MH  - Adipogenesis/genetics
MH  - Animals
MH  - CCAAT-Enhancer-Binding Protein-alpha/genetics
MH  - Cell Differentiation/genetics
MH  - Gene Expression Profiling
MH  - Mice
MH  - PPAR gamma/metabolism
MH  - *RNA, Long Noncoding/genetics/metabolism
MH  - Wnt Signaling Pathway/genetics
MH  - beta Catenin/genetics/metabolism
OTO - NOTNLM
OT  - Adipocyte differentiation
OT  - NR_015556
OT  - Wnt10b
OT  - beta-catenin
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2022/03/02 06:00
MHDA- 2022/04/12 06:00
CRDT- 2022/03/01 20:13
PHST- 2022/01/12 00:00 [received]
PHST- 2022/02/18 00:00 [accepted]
PHST- 2022/03/02 06:00 [pubmed]
PHST- 2022/04/12 06:00 [medline]
PHST- 2022/03/01 20:13 [entrez]
AID - S0006-291X(22)00259-5 [pii]
AID - 10.1016/j.bbrc.2022.02.069 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2022 Apr 23;601:79-85. doi: 
      10.1016/j.bbrc.2022.02.069. Epub 2022 Feb 22.