PMID- 35234362
OWN - NLM
STAT- MEDLINE
DCOM- 20220511
LR  - 20220516
IS  - 1615-9861 (Electronic)
IS  - 1615-9853 (Linking)
VI  - 22
IP  - 10
DP  - 2022 May
TI  - Effects of ABCG1 knockout on proteomic composition of HDL in mice on a chow diet 
      and a high-fat diet.
PG  - e2100028
LID - 10.1002/pmic.202100028 [doi]
AB  - ATP-binding cassette transporter G1 (ABCG1) is a cellular transmembrane protein 
      that transports oxysterol efflux from cells to high-density lipoprotein (HDL) 
      particles in the plasma. Previous studies have demonstrated that an ABCG1 
      deficiency exerts an antiatherosclerotic function through the effects of 
      oxysterol accumulation in cells to enhance apoptosis and regulate inflammatory 
      processes. However, whether the deficiency of ABCG1 and the corresponding changes 
      in the efflux of oxysterols could take a series of impacts on the proteomic 
      composition of HDL remains unclear. Here, plasma HDL of ABCG1(-/-) mice and their 
      wild-type controls on a normal chow diet (NCD) or a high-fat diet (HFD) were 
      isolated by ultracentrifugation. The proportion of 7-ketocholesterol and the 
      proteomic composition of samples were comparatively analyzed by LC-MS/MS. In 
      NCD-fed mice, lipid metabolism-related protein (arachidonate 12-lipoxygenase) and 
      antioxidative protein (pantetheinase) exhibited increased accumulation, and 
      inflammatory response protein (alpha-1-antitrypsin) was decreased in accumulation 
      in ABCG1(-/-) mice HDL. In HFD-fed mice, fewer proteins were detected than that 
      of NCD-fed mice. The ABCG1(-/-) mice HDL exhibited increased accumulation of 
      lipid metabolism-related proteins (e.g., carboxylesterase 1C, apolipoprotein 
      (apo)C-4) and decreased accumulation of alpha-1-antitrypsin, as well as 
      significantly reduced proportion of 7-ketocholesterol. Additionally, positive 
      correlations were found between 7-ketocholesterol and some essential proteins on 
      HDL, such as alpha-1-antitrypsin, apoA-4, apoB-100, and serum amyloid A (SAA). 
      These results suggest a detrimental impact of oxysterols on HDL composition, 
      which might affect the antiatherosclerotic properties of HDL.
CI  - (c) 2022 Wiley-VCH GmbH.
FAU - Wu, Yanxiang
AU  - Wu Y
AUID- ORCID: 0000-0001-5490-5719
AD  - Department of Cardiology, Peking Union Medical College Hospital, Chinese Academy 
      of Medical Sciences and Peking Union Medical College, Beijing, China.
FAU - Chen, Lianfeng
AU  - Chen L
AD  - Department of Cardiology, Peking Union Medical College Hospital, Chinese Academy 
      of Medical Sciences and Peking Union Medical College, Beijing, China.
FAU - Xie, Ziyan
AU  - Xie Z
AUID- ORCID: 0000-0002-1728-5001
AD  - Department of Cardiology, Peking Union Medical College Hospital, Chinese Academy 
      of Medical Sciences and Peking Union Medical College, Beijing, China.
FAU - Wang, Chenyu
AU  - Wang C
AUID- ORCID: 0000-0002-2416-2544
AD  - Department of Cardiology, Peking Union Medical College Hospital, Chinese Academy 
      of Medical Sciences and Peking Union Medical College, Beijing, China.
FAU - Zhang, Jiahao
AU  - Zhang J
AD  - Department of Cardiology, Peking Union Medical College Hospital, Chinese Academy 
      of Medical Sciences and Peking Union Medical College, Beijing, China.
FAU - Yan, Xiaowei
AU  - Yan X
AUID- ORCID: 0000-0003-4873-4006
AD  - Department of Cardiology, Peking Union Medical College Hospital, Chinese Academy 
      of Medical Sciences and Peking Union Medical College, Beijing, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220308
PL  - Germany
TA  - Proteomics
JT  - Proteomics
JID - 101092707
RN  - 0 (ABCG1 protein, mouse)
RN  - 0 (ATP Binding Cassette Transporter, Subfamily G, Member 1)
RN  - 0 (Lipoproteins)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily G, Member 1/genetics
MH  - Animals
MH  - Chromatography, Liquid
MH  - *Diet, High-Fat/adverse effects
MH  - Lipoproteins/metabolism
MH  - Mice
MH  - Mice, Knockout
MH  - *Noncommunicable Diseases
MH  - Proteomics
MH  - Tandem Mass Spectrometry
OTO - NOTNLM
OT  - ABCG1
OT  - HDL
OT  - atherosclerosis
OT  - oxysterol
OT  - proteomics
EDAT- 2022/03/03 06:00
MHDA- 2022/05/12 06:00
CRDT- 2022/03/02 08:43
PHST- 2022/02/18 00:00 [revised]
PHST- 2021/01/28 00:00 [received]
PHST- 2022/02/18 00:00 [accepted]
PHST- 2022/03/03 06:00 [pubmed]
PHST- 2022/05/12 06:00 [medline]
PHST- 2022/03/02 08:43 [entrez]
AID - 10.1002/pmic.202100028 [doi]
PST - ppublish
SO  - Proteomics. 2022 May;22(10):e2100028. doi: 10.1002/pmic.202100028. Epub 2022 Mar 
      8.