PMID- 35234378 OWN - NLM STAT- MEDLINE DCOM- 20220303 LR - 20220303 IS - 1872-2075 (Electronic) IS - 1000-3061 (Linking) VI - 38 IP - 2 DP - 2022 Feb 25 TI - [Advances of enzymes related to microbial cement]. PG - 506-517 LID - 10.13345/j.cjb.210127 [doi] AB - Microbial induced calcium carbonate precipitation (MICP) refers to the natural biological process of calcium carbonate precipitation induced by microbial metabolism in its surrounding environment. Based on the principles of MICP, microbial cement has been developed and has received widespread attention in the field of biology, civil engineering, and environment owing to the merits of environmental friendliness and economic competence. Urease and carbonic anhydrase are the key enzymes closely related to microbial cement. This review summarizes the genes, protein structures, regulatory mechanisms, engineering strains and mutual synergistic relationship of these two enzymes. The application of bioinformatics and synthetic biology is expected to develop biocement with a wide range of environmental adaptability and high performance, and will bring the MICP research to a new height. FAU - Wang, Lei AU - Wang L AD - School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, Hubei, China. AD - School of Chemical Engineering & Food Science, Zhengzhou University of Technology, Zhengzhou 450044, He'nan, China. FAU - Wang, Xuxia AU - Wang X AD - School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, Hubei, China. AD - School of Urban Construction, Wuchang Shouyi University, Wuhan 430064, Hubei, China. FAU - Li, Fei AU - Li F AD - School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, Hubei, China. FAU - Cui, Mingjuan AU - Cui M AD - School of Civil Engineering and Mechanics, Huazhong University of Science and Technology, Wuhan 430074, Hubei, China. FAU - Yang, Xiaoxu AU - Yang X AD - School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, Hubei, China. FAU - Yang, Min AU - Yang M AD - School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, Hubei, China. FAU - Yan, Yunjun AU - Yan Y AD - School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, Hubei, China. LA - chi PT - Journal Article PT - Review PL - China TA - Sheng Wu Gong Cheng Xue Bao JT - Sheng wu gong cheng xue bao = Chinese journal of biotechnology JID - 9426463 RN - EC 3.5.1.5 (Urease) RN - H0G9379FGK (Calcium Carbonate) SB - IM MH - *Calcium Carbonate/chemistry/metabolism MH - Chemical Precipitation MH - *Urease/genetics/metabolism OTO - NOTNLM OT - carbonic anhydrase OT - enzyme OT - microbial cement OT - microbial induced calcium carbonate precipitation (MICP) OT - urease EDAT- 2022/03/03 06:00 MHDA- 2022/03/04 06:00 CRDT- 2022/03/02 08:43 PHST- 2022/03/02 08:43 [entrez] PHST- 2022/03/03 06:00 [pubmed] PHST- 2022/03/04 06:00 [medline] AID - 10.13345/j.cjb.210127 [doi] PST - ppublish SO - Sheng Wu Gong Cheng Xue Bao. 2022 Feb 25;38(2):506-517. doi: 10.13345/j.cjb.210127.