PMID- 35236250
OWN - NLM
STAT- MEDLINE
DCOM- 20220408
LR  - 20220606
IS  - 2165-5987 (Electronic)
IS  - 2165-5979 (Print)
IS  - 2165-5979 (Linking)
VI  - 13
IP  - 3
DP  - 2022 Mar
TI  - Circular RNA ZNF609 promotes laryngeal squamous cell carcinoma progression by 
      upregulating epidermal growth factor receptor via sponging microRNA-134-5p.
PG  - 6929-6941
LID - 10.1080/21655979.2022.2034703 [doi]
AB  - Emerging evidence has revealed that aberrantly expressed circular RNAs (circRNAs) 
      play vital roles in tumorigenesis and progression of diverse human malignancies. 
      CircZNF609 was found to be involved in hepatocellular carcinoma, but the role and 
      underlying mechanism of circZNF609 in laryngeal squamous cell carcinoma (LSCC) 
      remain unclear. This study aimed to explore the molecular mechanism of circZNF609 
      in LSCC. qRT-qPCR was performed to detect the expression of circZNF609 and 
      microRNA-134-5p (miR-134-5p) in LSCC. Colony formation assay, CCK-8 assay, BrdU 
      incorporation assay, clone formation assay, transwell invasion assay and Western 
      blot analysis were performed to evaluate LSCC cell proliferation, as well as the 
      expression of proliferating cell nuclear antigen (PCNA) and MMP-2. Luciferase 
      reporter assay, target gene prediction and screening were used to validate 
      downstream target genes of circZNF609 and miR-134-5p. EGFR expression was 
      detected by Western blot analysis and RT-qPCR. Nude mice were used to detect 
      tumor changes. CircZNF609 was upregulated in LSCC and associated with poor 
      survival of LSCC patients. Knockdown of circZNF609 inhibited LSCC proliferation, 
      invasion and the expression of PCNA and matrix matalloproteinases-2 (MMP-2). 
      CircZNF609 can regulate miR-134-5p to upregulate epidermal growth factor receptor 
      (EGFR). In addition, knockdown of EGFR or overexpression of miR-134-5p could 
      reverse the tumor-promoting effects of circZNF609 in LSCC. In LSCC tissues, 
      circZNF609 was negatively correlated with miR-134-5p and positively correlated 
      with EGFR. CircZNF609 promotes the progression of LSCC via the miR-134-5p/EGFR 
      axis, which might be the therapeutic target of LSCC.
FAU - Yin, Xiaoyan
AU  - Yin X
AD  - Department of Otolaryngology, Head & Neck Surgery, The Second Hospital of Hebei 
      Medical University, PR. China.
FAU - Wang, Jingmiao
AU  - Wang J
AD  - Department of Otolaryngology, Head & Neck Surgery, The Second Hospital of Hebei 
      Medical University, PR. China.
FAU - Shan, Chunguang
AU  - Shan C
AD  - Department of Otolaryngology, Head & Neck Surgery, The Second Hospital of Hebei 
      Medical University, PR. China.
FAU - Jia, Qiaojing
AU  - Jia Q
AD  - Department of Otolaryngology, Head & Neck Surgery, The Second Hospital of Hebei 
      Medical University, PR. China.
FAU - Bian, Yanrui
AU  - Bian Y
AD  - Department of Otolaryngology, Head & Neck Surgery, The Second Hospital of Hebei 
      Medical University, PR. China.
FAU - Zhang, Haizhong
AU  - Zhang H
AUID- ORCID: 0000-0002-0836-180X
AD  - Department of Otolaryngology, Head & Neck Surgery, The Second Hospital of Hebei 
      Medical University, PR. China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Bioengineered
JT  - Bioengineered
JID - 101581063
RN  - 0 (MIRN134 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - 0 (RNA, Circular)
RN  - EC 2.7.10.1 (ErbB Receptors)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
SB  - IM
MH  - Animals
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - ErbB Receptors/genetics/metabolism
MH  - Gene Expression Regulation, Neoplastic
MH  - *Head and Neck Neoplasms/genetics
MH  - Humans
MH  - *Laryngeal Neoplasms/genetics/metabolism/pathology
MH  - *Liver Neoplasms/genetics
MH  - Matrix Metalloproteinase 2/genetics
MH  - Mice
MH  - Mice, Nude
MH  - *MicroRNAs/genetics/metabolism
MH  - Proliferating Cell Nuclear Antigen/genetics/metabolism
MH  - RNA, Circular/genetics
MH  - Squamous Cell Carcinoma of Head and Neck/genetics
PMC - PMC8973624
OTO - NOTNLM
OT  - CircRNAs
OT  - EGFR
OT  - ZNF609
OT  - laryngeal squamous cell carcinoma
OT  - miR-134-5p
COIS- No potential conflict of interest was reported by the author(s).
EDAT- 2022/03/04 06:00
MHDA- 2022/04/09 06:00
PMCR- 2022/03/02
CRDT- 2022/03/03 05:30
PHST- 2022/03/03 05:30 [entrez]
PHST- 2022/03/04 06:00 [pubmed]
PHST- 2022/04/09 06:00 [medline]
PHST- 2022/03/02 00:00 [pmc-release]
AID - 2034703 [pii]
AID - 10.1080/21655979.2022.2034703 [doi]
PST - ppublish
SO  - Bioengineered. 2022 Mar;13(3):6929-6941. doi: 10.1080/21655979.2022.2034703.