PMID- 35239213
OWN - NLM
STAT- MEDLINE
DCOM- 20220422
LR  - 20220422
IS  - 1530-6860 (Electronic)
IS  - 0892-6638 (Linking)
VI  - 36
IP  - 4
DP  - 2022 Apr
TI  - Tumor microenvironment remodeling modulates macrophage phenotype in breast cancer 
      lymphangiogenesis.
PG  - e22248
LID - 10.1096/fj.202101230R [doi]
AB  - Hyaluronan (HA) is dynamically remodeled in tumor microenvironment (TME) and is 
      reported to be closely related to tumor lymphatic metastasis by inducing 
      lymphangiogenesis. Macrophages are known to be involved in neo-lymphatic vessels 
      formation. However, few studies have investigated the role of HA-mediated TME 
      remodeling on macrophages-dependent lymphangiogenesis. We previously showed that 
      HA could drive macrophages to acquire the M2 phenotype. In this study, we attempt 
      to study the crosstalk between HA in TME and macrophages dependent 
      lymphangiogenesis. First, we found that the abundant assembly of HA in breast 
      cancer tissue was accompanied by increased infiltration of macrophages featured 
      by expressing lymphatic endothelial markers. Then, to further identify the 
      remodeling of HA in regulating macrophage phenotype, we used HA fragments which 
      are usually enriched in TME for this purpose. Our results showed that the 
      reconstructed HA could induce bone marrow-derived macrophages (BMDMs) to express 
      markers of lymphatic endothelium and form tube-like structures, suggesting a 
      novel function of HA from TME on macrophages-dependent lymphangiogenesis. 
      Finally, we found that inhibition of the HA-TLR4 pathway could reduce the ability 
      of BMDMs to exhibit lymphatic endothelial phenotype. Our results provide new 
      insight into tumor microenvironment remodeling and macrophages in breast cancer 
      lymphangiogenesis.
CI  - (c) 2022 Federation of American Societies for Experimental Biology.
FAU - Du, Yan
AU  - Du Y
AD  - Department of Molecular Biology, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai, P.R. China.
AD  - Department of Clinical Laboratory, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai, P.R. China.
FAU - Cao, Manlin
AU  - Cao M
AD  - Department of Rehabilitation Medicine, Shanghai Jiao Tong University Affiliated 
      Sixth People's Hospital, Shanghai, P.R. China.
FAU - Liu, Yiwen
AU  - Liu Y
AD  - Department of Molecular Biology, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai, P.R. China.
FAU - He, Yiqing
AU  - He Y
AD  - Department of Molecular Biology, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai, P.R. China.
FAU - Yang, Cuixia
AU  - Yang C
AD  - Department of Molecular Biology, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai, P.R. China.
AD  - Department of Clinical Laboratory, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai, P.R. China.
FAU - Zhang, Guoliang
AU  - Zhang G
AD  - Department of Molecular Biology, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai, P.R. China.
FAU - Fan, Youben
AU  - Fan Y
AD  - Department of General Surgery, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai, P.R. China.
FAU - Gao, Feng
AU  - Gao F
AD  - Department of Molecular Biology, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai, P.R. China.
AD  - Department of Clinical Laboratory, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - FASEB J
JT  - FASEB journal : official publication of the Federation of American Societies for 
      Experimental Biology
JID - 8804484
RN  - 9004-61-9 (Hyaluronic Acid)
SB  - IM
MH  - *Breast Neoplasms/metabolism
MH  - Female
MH  - Humans
MH  - Hyaluronic Acid/metabolism
MH  - Lymphangiogenesis
MH  - *Lymphatic Vessels/metabolism
MH  - Macrophages/metabolism
MH  - Phenotype
MH  - Tumor Microenvironment
OTO - NOTNLM
OT  - LMW-HA
OT  - bone marrow-derived macrophages
OT  - hyaluronan remodeling
OT  - lymphangiogenesis
OT  - tumor microenvironment
EDAT- 2022/03/04 06:00
MHDA- 2022/04/23 06:00
CRDT- 2022/03/03 12:20
PHST- 2022/02/11 00:00 [revised]
PHST- 2021/08/02 00:00 [received]
PHST- 2022/02/24 00:00 [accepted]
PHST- 2022/03/03 12:20 [entrez]
PHST- 2022/03/04 06:00 [pubmed]
PHST- 2022/04/23 06:00 [medline]
AID - 10.1096/fj.202101230R [doi]
PST - ppublish
SO  - FASEB J. 2022 Apr;36(4):e22248. doi: 10.1096/fj.202101230R.