PMID- 35243180
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240210
IS  - 2452-073X (Electronic)
IS  - 2452-073X (Linking)
VI  - 11
DP  - 2022 Jan-Jul
TI  - Differential DNA methylation in Black and White individuals with chronic low back 
      pain enrich different genomic pathways.
PG  - 100086
LID - 10.1016/j.ynpai.2022.100086 [doi]
LID - 100086
AB  - Compared to Non-Hispanic Whites (NHWs), individuals who self-identify as 
      Non-Hispanic Blacks (NHBs) in the United States experience more severe and 
      disabling chronic low back pain (cLBP). We hypothesized that differences in DNA 
      methylation (DNAm) play a role in racial disparities in cLBP. PURPOSE: To 
      determine the relationship between DNAm levels and racial group differences in 
      adults with cLBP. Our study's secondary purpose was to perform a race-stratified 
      comparison of adults with cLBP and pain-free controls and identify functional 
      genomic pathways enriched by annotated differentially methylated genes. PATIENTS 
      AND METHODS: We recruited 49 NHBs and 49 NHWs (49 cLBP and 49 pain-free controls, 
      PFCs), analyzed DNAm from whole blood using reduced representation bisulfite 
      sequencing, and identified enriched genomic pathways. RESULTS: Among participants 
      with cLBP, we identified 2873 differentially methylated loci (DML; methylation 
      differences of at least 10% and p < 0.0001), many of which were annotated to 
      genes of importance to pain pathology. These DMLs significantly enriched pathways 
      to involved in nociception/pain processing (Dopamine-DARPP32 Feedback in cAMP 
      signaling, GABA Receptor Signaling, Opioid Signaling) and neuronal 
      differentiation (e.g., Calcium Signaling, Axon Guidance Signaling, and 
      Endocannabinoid Neuronal Synapse). Our race stratified analyses of individuals 
      with cLBP versus PFCs revealed 2356 DMLs in NHBs and 772 DMLs in NHWs with 
      p < 0.0001 and > 10% methylation difference. Ingenuity Pathway Analysis revealed 
      that many pathways of significance to pain such as Corticotropin Releasing 
      Hormone Signaling, White Adipose Tissue Browning, and GABA Receptor Signaling 
      pathways, were more significant in NHBs than NHWs. CONCLUSION: Even though an 
      individual's self-identified race is a social construct, not a biological 
      variable, racism associated with that classification affects virtually every 
      aspect of life, including disease risk. DNAm induced alterations in stress 
      signaling pathways may explain worse pain outcomes in NHBs.
CI  - (c) 2022 The Author(s).
FAU - Aroke, Edwin N
AU  - Aroke EN
AD  - School of Nursing, University of Alabama at Birmingham, Birmingham, AL, USA.
FAU - Jackson, Pamela
AU  - Jackson P
AD  - School of Nursing, University of Alabama at Birmingham, Birmingham, AL, USA.
FAU - Meng, Lingsong
AU  - Meng L
AD  - Department of Biostatistics, University of Florida, Gainesville, FL, USA.
FAU - Huo, Zhiguang
AU  - Huo Z
AD  - Department of Biostatistics, University of Florida, Gainesville, FL, USA.
FAU - Overstreet, Demario S
AU  - Overstreet DS
AD  - Department of Psychology, University of Alabama at Birmingham, Birmingham, AL, 
      USA.
FAU - Penn, Terence M
AU  - Penn TM
AD  - Department of Psychology, University of Alabama at Birmingham, Birmingham, AL, 
      USA.
FAU - Quinn, Tammie L
AU  - Quinn TL
AD  - Department of Psychology, University of Alabama at Birmingham, Birmingham, AL, 
      USA.
FAU - Cruz-Almeida, Yenisel
AU  - Cruz-Almeida Y
AD  - College of Dentistry, University of Florida, Gainesville, FL, USA.
AD  - Pain Research & Intervention Center of Excellence, University of Florida, 
      Gainesville, FL, USA.
FAU - Goodin, Burel R
AU  - Goodin BR
AD  - Department of Psychology, University of Alabama at Birmingham, Birmingham, AL, 
      USA.
LA  - eng
GR  - P30 AG028740/AG/NIA NIH HHS/United States
GR  - R01 AR079178/AR/NIAMS NIH HHS/United States
GR  - R01 MD010441/MD/NIMHD NIH HHS/United States
GR  - T32 HS013852/HS/AHRQ HHS/United States
PT  - Journal Article
DEP - 20220225
PL  - United States
TA  - Neurobiol Pain
JT  - Neurobiology of pain (Cambridge, Mass.)
JID - 101705141
PMC - PMC8885563
OTO - NOTNLM
OT  - Chronic low back pain
OT  - DML, DIfferentially methylated loci
OT  - DNA methylation
OT  - DNAm, DNA methylation
OT  - Epigenetics
OT  - NHB, Non-Hispanic Black
OT  - NHW, Non-Hispanic White
OT  - Non-specific chronic low back pain
OT  - PFC, Pain free control
OT  - Pain mechanisms
OT  - RRBS
OT  - RRBS, Reduced representation bisulfite sequencing
OT  - Racial pain disparities
OT  - cLBP, Chronic low back pain
COIS- The authors declare that they have no known competing financial interests or 
      personal relationships that could have appeared to influence the work reported in 
      this paper.
EDAT- 2022/03/05 06:00
MHDA- 2022/03/05 06:01
PMCR- 2022/02/25
CRDT- 2022/03/04 05:43
PHST- 2022/01/10 00:00 [received]
PHST- 2022/02/07 00:00 [revised]
PHST- 2022/02/20 00:00 [accepted]
PHST- 2022/03/04 05:43 [entrez]
PHST- 2022/03/05 06:00 [pubmed]
PHST- 2022/03/05 06:01 [medline]
PHST- 2022/02/25 00:00 [pmc-release]
AID - S2452-073X(22)00003-4 [pii]
AID - 100086 [pii]
AID - 10.1016/j.ynpai.2022.100086 [doi]
PST - epublish
SO  - Neurobiol Pain. 2022 Feb 25;11:100086. doi: 10.1016/j.ynpai.2022.100086. 
      eCollection 2022 Jan-Jul.