PMID- 35246006
OWN - NLM
STAT- MEDLINE
DCOM- 20220408
LR  - 20220606
IS  - 2165-5987 (Electronic)
IS  - 2165-5979 (Print)
IS  - 2165-5979 (Linking)
VI  - 13
IP  - 3
DP  - 2022 Mar
TI  - Bone marrow mesenchymal stem cells-derived exosomes suppress miRNA-5189-3p to 
      increase fibroblast-like synoviocyte apoptosis via the BATF2/JAK2/STAT3 signaling 
      pathway.
PG  - 6767-6780
LID - 10.1080/21655979.2022.2045844 [doi]
AB  - Ankylosing spondylitis (AS) is characterized by inflammation of the sacroiliac 
      joint and the attachment point of the spine. Herein, we aimed to investigate the 
      effect of bone marrow mesenchymal stem cells (BMSCs)-derived exosomes on 
      apoptosis of fibroblast-like synoviocytes (FLSs) and explored its molecular 
      mechanism. Exosomes were isolated from BMSCs and verified by transmission 
      electron microscope and nanoparticle tracking analysis. FLSs were isolated and 
      co-incubated with BMSC exosomes. Cell apoptosis was assessed using terminal 
      deoxynucleotidyl transferase-mediated dUTP nick-end labeling analysis and flow 
      cytometry. The results showed that BMSC exosomes increased apoptosis of FLSs. 
      MiR-5189-3p was downregulated, while basic leucine zipper transcription factor 
      ATF-like 2 (BATF2) was upregulated in FLSs by treatment of BMSC exosomes. As a 
      direct target of miR-5189-3p, BATF2 inactivates the JAK2/STAT3 pathway. 
      MiR-5189-3p suppressed apoptosis of FLSs and BATF2 exerted an opposite effect. In 
      conclusion, BMSCs-derived exosomes suppress miR-5189-3p to facilitate the 
      apoptosis of FLSs via the BATF2/JAK2/STAT3 signaling pathway, which facilitates 
      the understanding of the therapeutic effect of BMSCs on AS and the underlying 
      molecular mechanism.
FAU - Zhang, Yiqun
AU  - Zhang Y
AD  - Department of Spine Surgery, First Affiliated Hospital of Anhui Medical 
      University, Hefei, Anhui, China.
FAU - Tu, Bizhi
AU  - Tu B
AD  - Department of Spine Surgery, First Affiliated Hospital of Anhui Medical 
      University, Hefei, Anhui, China.
FAU - Sha, Qi
AU  - Sha Q
AD  - Department of Spine Surgery, First Affiliated Hospital of Anhui Medical 
      University, Hefei, Anhui, China.
FAU - Qian, Jun
AU  - Qian J
AUID- ORCID: 0000-0003-2284-9323
AD  - Department of Spine Surgery, First Affiliated Hospital of Anhui Medical 
      University, Hefei, Anhui, China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Bioengineered
JT  - Bioengineered
JID - 101581063
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Apoptosis/genetics
MH  - Cell Proliferation
MH  - *Exosomes/genetics/metabolism
MH  - Fibroblasts/metabolism
MH  - *Mesenchymal Stem Cells/metabolism
MH  - *MicroRNAs/genetics/metabolism
MH  - Signal Transduction/genetics
MH  - *Synoviocytes/metabolism
PMC - PMC8973596
OTO - NOTNLM
OT  - Ankylosing spondylitis
OT  - BATF2
OT  - JAK2/STAT3/signaling
OT  - bone marrow mesenchymal stem cells
OT  - exosome
OT  - fibroblast-like synoviocytes
OT  - miR-5189-3p
COIS- No potential conflict of interest was reported by the author(s).
EDAT- 2022/03/06 06:00
MHDA- 2022/04/09 06:00
PMCR- 2022/03/04
CRDT- 2022/03/05 05:24
PHST- 2022/03/05 05:24 [entrez]
PHST- 2022/03/06 06:00 [pubmed]
PHST- 2022/04/09 06:00 [medline]
PHST- 2022/03/04 00:00 [pmc-release]
AID - 2045844 [pii]
AID - 10.1080/21655979.2022.2045844 [doi]
PST - ppublish
SO  - Bioengineered. 2022 Mar;13(3):6767-6780. doi: 10.1080/21655979.2022.2045844.