PMID- 35246248
OWN - NLM
STAT- MEDLINE
DCOM- 20220321
LR  - 20220321
IS  - 2047-783X (Electronic)
IS  - 0949-2321 (Print)
IS  - 0949-2321 (Linking)
VI  - 27
IP  - 1
DP  - 2022 Mar 5
TI  - Identification of diagnostic markers and immune cell infiltration characteristics 
      in antineutrophil cytoplasmic antibody-associated vasculitis by weighted gene 
      co-expression network analysis.
PG  - 37
LID - 10.1186/s40001-022-00666-3 [doi]
LID - 37
AB  - BACKGROUND: Antineutrophil cytoplasmic antibody-associated vasculitis (AAV) is a 
      group of life-threatening systemic autoimmune diseases. The aim of this study was 
      to determine the relationship between the AAV hub gene and immune cell 
      infiltration, and its value for clinical disease treatment. METHODS: We 
      downloaded the microarray information of 37 AAV patients and 27 controls from 
      Gene Expression Omnibus (GEO). Genes were classified into totally different 
      modules exploitation weighted gene co-expression network analysis (WGCNA). AAV 
      diagnostic indicators were screened and then assessed immune cell infiltration by 
      the least absolute shrinkage and selection operator (LASSO) and CIBERSORT. 
      Finally, Connectivity Map analysis was applied to predict possible AAV glomerulus 
      injury improvement therapies. RESULTS: WGCNA was developed and differentially 
      expressed genes were classified into 6 modules, the black module was most tightly 
      correlated to AAV. Among them, TIMP1 and FCER1G were most closely related to 
      clinical features. Resting mast cells and monocytes emerged as having the 
      foremost distinguished variations in AAV. C3AR1 and FCER1G were involved in AAV 
      development by immune regulation. Connectivity Map analysis indicated the most 
      significant compound was fisetin. CONCLUSIONS: The present study is that the 
      initial to spot immune cell infiltration with microarray data of glomeruli in 
      AAV, which provides novel proof and clues for additional analysis of the 
      molecular mechanisms.
CI  - (c) 2022. The Author(s).
FAU - Xia, Mengdi
AU  - Xia M
AD  - Nanchong Key Laboratory of Basic Science & Clinical Research On Chronic Kidney 
      Disease, Department of Nephrology, The Second Clinical Medical Institution of 
      North Sichuan Medical College (Nanchong Central Hospital), Nanchong, China.
FAU - Zhao, Fen
AU  - Zhao F
AD  - Shanxi Kidney Disease Institute, Department of Nephrology, Shanxi Provincial 
      People's Hospital, The Affiliated People's Hospital of Shanxi Medical University, 
      Taiyuan, China.
FAU - Zhang, Yongji
AU  - Zhang Y
AD  - Shanxi Kidney Disease Institute, Department of Nephrology, Shanxi Provincial 
      People's Hospital, The Affiliated People's Hospital of Shanxi Medical University, 
      Taiyuan, China.
FAU - Zheng, Zhihuang
AU  - Zheng Z
AD  - Department of Nephrology, Shanghai General Hospital, Shanghai Jiaotong University 
      School of Medicine, Shanghai, China.
FAU - Zhou, Yun
AU  - Zhou Y
AD  - Shanxi Kidney Disease Institute, Department of Nephrology, Shanxi Provincial 
      People's Hospital, The Affiliated People's Hospital of Shanxi Medical University, 
      Taiyuan, China. zhouyun_sx@163.com.
FAU - Liu, Tong
AU  - Liu T
AD  - Shanxi Kidney Disease Institute, Department of Nephrology, Shanxi Provincial 
      People's Hospital, The Affiliated People's Hospital of Shanxi Medical University, 
      Taiyuan, China. tong.liu@charite.de.
LA  - eng
PT  - Journal Article
DEP - 20220305
PL  - England
TA  - Eur J Med Res
JT  - European journal of medical research
JID - 9517857
RN  - 0 (Antibodies, Antineutrophil Cytoplasmic)
RN  - 0 (Biomarkers)
RN  - 0 (KIAA0319L protein, human)
RN  - 0 (Receptors, Cell Surface)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Anti-Neutrophil Cytoplasmic Antibody-Associated 
      Vasculitis/*diagnosis/genetics/metabolism
MH  - Antibodies, Antineutrophil Cytoplasmic/*genetics/immunology
MH  - Autoimmunity/*genetics
MH  - Biomarkers/metabolism
MH  - *Gene Expression Regulation
MH  - Gene Regulatory Networks
MH  - Humans
MH  - Kidney Glomerulus/*immunology/pathology
MH  - RNA/*genetics
MH  - Receptors, Cell Surface/biosynthesis/*genetics
PMC - PMC8897842
OTO - NOTNLM
OT  - ANCA-associated glomerulonephritis
OT  - Antineutrophil cytoplasmic antibody-associated vasculitis (AAV)
OT  - CIBERSORT
OT  - Hub gene
OT  - Immune infiltration
COIS- The authors declare no conflict of interest.
EDAT- 2022/03/06 06:00
MHDA- 2022/03/22 06:00
PMCR- 2022/03/05
CRDT- 2022/03/05 05:25
PHST- 2021/11/03 00:00 [received]
PHST- 2022/02/23 00:00 [accepted]
PHST- 2022/03/05 05:25 [entrez]
PHST- 2022/03/06 06:00 [pubmed]
PHST- 2022/03/22 06:00 [medline]
PHST- 2022/03/05 00:00 [pmc-release]
AID - 10.1186/s40001-022-00666-3 [pii]
AID - 666 [pii]
AID - 10.1186/s40001-022-00666-3 [doi]
PST - epublish
SO  - Eur J Med Res. 2022 Mar 5;27(1):37. doi: 10.1186/s40001-022-00666-3.