PMID- 35247842
OWN - NLM
STAT- MEDLINE
DCOM- 20220415
LR  - 20220415
IS  - 1876-7753 (Electronic)
IS  - 1873-5061 (Linking)
VI  - 60
DP  - 2022 Apr
TI  - Establishment of human embryonic stem cell WAe009-A-74 carrying a Long QT 
      syndrome mutation in KCNH2.
PG  - 102725
LID - S1873-5061(22)00074-5 [pii]
LID - 10.1016/j.scr.2022.102725 [doi]
AB  - Long-QT syndrome type 2 (LQT2) is a common malignant hereditary arrhythmia. Due 
      to the lack of suitable animal and human models, the pathogenesis of LQT2 caused 
      by human ether-a-go-go-related gene (hERG) deficiency is still unclear. Herein, 
      we have generated a human embryonic stem cell line (WAe009-A-74) carrying a LQTS 
      related mutation in KCNH2. The WAe009-A-74 line maintained stem cell like 
      morphology, pluripotency, normal karyotype and could differentiate into all three 
      germ layers in vivo.
CI  - Copyright (c) 2022 The Authors. Published by Elsevier B.V. All rights reserved.
FAU - Sun, Lixiang
AU  - Sun L
AD  - Guangxi Key Laboratory of Precision Medicine in Cardio-cerebrovascular Diseases 
      Control and Prevention & Guangxi Clinical Research Center for 
      Cardio-cerebrovascular Diseases, Nanning, Guangxi, China; Elderly Cardiology 
      Ward, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, 
      China.
FAU - Zhong, Jiaqi
AU  - Zhong J
AD  - Translational Medicine Collaborative Innovation Center, The Second Clinical 
      Medical College (Shenzhen People's Hospital), Jinan University, 518020 Shenzhen, 
      China; Shenzhen Key Laboratory of Stem Cell Research and Clinical Transformation, 
      518020 Shenzhen, China.
FAU - Wu, Fujian
AU  - Wu F
AD  - Translational Medicine Collaborative Innovation Center, The Second Clinical 
      Medical College (Shenzhen People's Hospital), Jinan University, 518020 Shenzhen, 
      China; Shenzhen Key Laboratory of Stem Cell Research and Clinical Transformation, 
      518020 Shenzhen, China; Guangdong Engineering Technology Research Center of Stem 
      Cell and Cell Therapy, 518020 Shenzhen, China.
FAU - Li, Furong
AU  - Li F
AD  - Translational Medicine Collaborative Innovation Center, The Second Clinical 
      Medical College (Shenzhen People's Hospital), Jinan University, 518020 Shenzhen, 
      China; Shenzhen Key Laboratory of Stem Cell Research and Clinical Transformation, 
      518020 Shenzhen, China; Guangdong Engineering Technology Research Center of Stem 
      Cell and Cell Therapy, 518020 Shenzhen, China.
FAU - Yang, Xiaofei
AU  - Yang X
AD  - Translational Medicine Collaborative Innovation Center, The Second Clinical 
      Medical College (Shenzhen People's Hospital), Jinan University, 518020 Shenzhen, 
      China; Shenzhen Key Laboratory of Stem Cell Research and Clinical Transformation, 
      518020 Shenzhen, China; Guangdong Engineering Technology Research Center of Stem 
      Cell and Cell Therapy, 518020 Shenzhen, China. Electronic address: 
      sophiayangxf@163.com.
FAU - Zeng, Zhiyu
AU  - Zeng Z
AD  - Guangxi Key Laboratory of Precision Medicine in Cardio-cerebrovascular Diseases 
      Control and Prevention & Guangxi Clinical Research Center for 
      Cardio-cerebrovascular Diseases, Nanning, Guangxi, China; Elderly Cardiology 
      Ward, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, 
      China. Electronic address: zhiyuzeng@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220301
PL  - England
TA  - Stem Cell Res
JT  - Stem cell research
JID - 101316957
RN  - 0 (ERG1 Potassium Channel)
RN  - 0 (KCNH2 protein, human)
SB  - IM
MH  - Animals
MH  - Arrhythmias, Cardiac
MH  - ERG1 Potassium Channel/genetics
MH  - *Human Embryonic Stem Cells
MH  - Humans
MH  - *Long QT Syndrome/genetics
MH  - Mutation/genetics
EDAT- 2022/03/06 06:00
MHDA- 2022/04/16 06:00
CRDT- 2022/03/05 20:18
PHST- 2021/11/08 00:00 [received]
PHST- 2022/01/07 00:00 [revised]
PHST- 2022/02/23 00:00 [accepted]
PHST- 2022/03/06 06:00 [pubmed]
PHST- 2022/04/16 06:00 [medline]
PHST- 2022/03/05 20:18 [entrez]
AID - S1873-5061(22)00074-5 [pii]
AID - 10.1016/j.scr.2022.102725 [doi]
PST - ppublish
SO  - Stem Cell Res. 2022 Apr;60:102725. doi: 10.1016/j.scr.2022.102725. Epub 2022 Mar 
      1.