PMID- 35248622
OWN - NLM
STAT- MEDLINE
DCOM- 20220404
LR  - 20220405
IS  - 1873-6424 (Electronic)
IS  - 0269-7491 (Linking)
VI  - 302
DP  - 2022 Jun 1
TI  - Autophagic event and metabolomic disorders unveil cellular toxicity of 
      environmental microplastics on marine polychaete Hediste diversicolor.
PG  - 119106
LID - S0269-7491(22)00320-7 [pii]
LID - 10.1016/j.envpol.2022.119106 [doi]
AB  - Although the hazards of microplastics (MPs) have been quite well explored, the 
      aberrant metabolism and the involvement of the autophagy pathway as an adverse 
      response to environmental MPs in benthic organisms are still unclear. The present 
      work aims to assess the impact of different environmental MPs collected from the 
      south coast of the Mediterranean Sea, composed by polyethylene (PE), polyethylene 
      vinyl acetate (PEVA), low-density polyethylene (LDPE), high-density polyethylene 
      (HDPE), polypropylene (PP) and polyamide (PA) on the metabolome and proteome of 
      the marine polychaete Hediste diversicolor. As a result, all the microplastic 
      types were detected with Raman microspectroscopy in polychaetes tissues, causing 
      cytoskeleton damage and induced autophagy pathway manifested by 
      immunohistochemical labeling of specific targeted proteins, through Tubulin 
      (Tub), Microtubule-associated protein light chain 3 (LC3), and p62 (also named 
      Sequestosome 1). Metabolomics was conducted to further investigate the metabolic 
      alterations induced by the environmental MPs-mixture in polychaetes. A total of 
      28 metabolites were differentially expressed between control and MPs-treated 
      polychaetes, which showed elevated levels of amino acids, glucose, ATP/ADP, 
      osmolytes, glutathione, choline and phosphocholine, and reduced concentration of 
      aspartate. These novel findings extend our understanding given the toxicity of 
      environmental microplastics and unravel their underlying mechanisms.
CI  - Copyright (c) 2022 Elsevier Ltd. All rights reserved.
FAU - Missawi, Omayma
AU  - Missawi O
AD  - University of Sousse, Laboratory of Agrobiodiversity and Ecotoxicology, Higher 
      Institute of Agronomy, Sousse, Tunisia. Electronic address: 
      missawi_omayma@yahoo.fr.
FAU - Venditti, Massimo
AU  - Venditti M
AD  - Department of Experimental Medicine, Section Human Physiology and Integrated 
      Biological Functions "F. Bottazzi", University of Campania "Luigi Vanvitelli", 
      Naples, Italy.
FAU - Cappello, Tiziana
AU  - Cappello T
AD  - University of Messina, Department of Chemical, Biological, Pharmaceutical and 
      Environmental Sciences, 98166 Messina, Italy.
FAU - Zitouni, Nesrine
AU  - Zitouni N
AD  - University of Sousse, Laboratory of Agrobiodiversity and Ecotoxicology, Higher 
      Institute of Agronomy, Sousse, Tunisia.
FAU - Marco, Giuseppe DE
AU  - Marco G
AD  - University of Messina, Department of Chemical, Biological, Pharmaceutical and 
      Environmental Sciences, 98166 Messina, Italy.
FAU - Boughattas, Iteb
AU  - Boughattas I
AD  - University of Sousse, Laboratory of Agrobiodiversity and Ecotoxicology, Higher 
      Institute of Agronomy, Sousse, Tunisia; Regional Field Crops Research Center of 
      Beja, Tunisia.
FAU - Bousserrhine, Noureddine
AU  - Bousserrhine N
AD  - University Paris-Est Creteil, Laboratory of Water, Environment and Urban Systems, 
      Faculty of Science and Technology, Creteil Cedex, France.
FAU - Belbekhouche, Sabrina
AU  - Belbekhouche S
AD  - CNRS, University of Paris-Est Creteil, Institute of Chemistry and Materials 
      Paris-Est ICMPE, UMR7182, 94320 Thiais, France.
FAU - Minucci, Sergio
AU  - Minucci S
AD  - Department of Experimental Medicine, Section Human Physiology and Integrated 
      Biological Functions "F. Bottazzi", University of Campania "Luigi Vanvitelli", 
      Naples, Italy.
FAU - Maisano, Maria
AU  - Maisano M
AD  - University of Messina, Department of Chemical, Biological, Pharmaceutical and 
      Environmental Sciences, 98166 Messina, Italy.
FAU - Banni, Mohamed
AU  - Banni M
AD  - University of Sousse, Laboratory of Agrobiodiversity and Ecotoxicology, Higher 
      Institute of Agronomy, Sousse, Tunisia; Higher Institute of Biotechnology 
      Monastir, University of Monastir, Monastir, Tunisia.
LA  - eng
PT  - Journal Article
DEP - 20220303
PL  - England
TA  - Environ Pollut
JT  - Environmental pollution (Barking, Essex : 1987)
JID - 8804476
RN  - 0 (Microplastics)
RN  - 0 (Plastics)
RN  - 0 (Water Pollutants, Chemical)
RN  - 9002-88-4 (Polyethylene)
SB  - IM
MH  - Autophagy
MH  - Environmental Monitoring
MH  - Metabolomics
MH  - *Microplastics
MH  - Plastics/toxicity
MH  - Polyethylene
MH  - *Water Pollutants, Chemical/analysis/toxicity
OTO - NOTNLM
OT  - Autophagy
OT  - Cytoskeleton
OT  - Environmental microplastic
OT  - Metabolome
OT  - Polychaete
EDAT- 2022/03/07 06:00
MHDA- 2022/04/05 06:00
CRDT- 2022/03/06 20:29
PHST- 2021/12/20 00:00 [received]
PHST- 2022/02/26 00:00 [revised]
PHST- 2022/03/03 00:00 [accepted]
PHST- 2022/03/07 06:00 [pubmed]
PHST- 2022/04/05 06:00 [medline]
PHST- 2022/03/06 20:29 [entrez]
AID - S0269-7491(22)00320-7 [pii]
AID - 10.1016/j.envpol.2022.119106 [doi]
PST - ppublish
SO  - Environ Pollut. 2022 Jun 1;302:119106. doi: 10.1016/j.envpol.2022.119106. Epub 
      2022 Mar 3.