PMID- 35249280
OWN - NLM
STAT- MEDLINE
DCOM- 20220308
LR  - 20220531
IS  - 0529-5807 (Print)
IS  - 0529-5807 (Linking)
VI  - 51
IP  - 3
DP  - 2022 Mar 8
TI  - [Diagnostic value of MDM2 RNA in situ hybridization in atypical lipomatous 
      tumor/well-differentiated liposarcoma and dedifferentiated liposarcoma].
PG  - 190-195
LID - 10.3760/cma.j.cn112151-20211029-00785 [doi]
AB  - Objective: To investigate the value of MDM2 RNA in situ hybridization (RNA-ISH) 
      in diagnosing atypical lipomatous tumor/well-differentiated liposarcoma (ALT/WDL) 
      and dedifferentiated liposarcoma (DDL). Methods: A total of 26 ALT/WDL/DDLs 
      diagnosed from March 2017 to May 2019 in West China Hospital, Sichuan University, 
      Chengdu, China and 18 control cases were included. MDM2 RNA-ISH was performed on 
      all samples and compared with the fluorescence in situ hybridization (FISH) and 
      immunohistochemistry (IHC) regarding their performance in detecting MDM2. 
      Results: All samples were detected successfully using the three methods. Among 26 
      ALT/WDL/DDLs, all cases showed MDM2 amplification and positivity for MDM2 RNA-ISH 
      (26/26, 100%). Twenty-four (24/26, 92.3%) of the 26 tested cases were positive 
      for MDM2 IHC while two of them were negative. Eighteen control cases were all 
      negative for MDM2 FISH and RNA-ISH, and 15 (15/18) cases were negative for MDM2 
      IHC. The sensitivity and specificity of RNA-ISH were both 100%, and those of MDM2 
      IHC were 92.3% and 83.3%, respectively. Diffuse staining was identified in all 
      MDM2 RNA-ISH positive ALT/WDL/DDLs, but identified in only 8/24 (33.3%) of the 
      MDM2 IHC positive cases. Among the 11 ALT/WDL/DDL samples evaluated on tissue 
      microarray, the positive rate of MDM2 RNA-ISH was 100% with diffuse staining in 
      all cases. The positive rate of MDM2 IHC was 9/11 while only 1 of the 9 cases 
      showed diffuse staining. The result of MDM2 RNA-ISH was identical to that of MDM2 
      FISH and was overall consistent with that of MDM2 IHC (Kappa=0.763, P<0.001). 
      Conclusions: In ALT/WDL/DDLs, results of MDM2 RNA-ISH are highly consistent with 
      those of FISH. MDM2 RNA-ISH is more sensitive and more specific and has more 
      diffuse positive signals than the IHC. The findings indicate that MDM2 RNA-ISH is 
      highly valuable for the diagnosis and differential diagnosis of ALT/WDL/DDLs.
FAU - Chen, C
AU  - Chen C
AD  - Department of Pathology, West China Hospital, Sichuan University, Chengdu 610041, 
      China.
FAU - He, X
AU  - He X
AD  - Department of Pathology, West China Hospital, Sichuan University, Chengdu 610041, 
      China.
FAU - Jing, W Y
AU  - Jing WY
AD  - Department of Pathology, West China Hospital, Sichuan University, Chengdu 610041, 
      China.
FAU - Qiu, Y
AU  - Qiu Y
AD  - Department of Pathology, West China Hospital, Sichuan University, Chengdu 610041, 
      China.
FAU - Chen, M
AU  - Chen M
AD  - Department of Pathology, West China Hospital, Sichuan University, Chengdu 610041, 
      China.
FAU - Luo, T Y
AU  - Luo TY
AD  - Department of Pathology, West China Hospital, Sichuan University, Chengdu 610041, 
      China.
FAU - Liu, X Y
AU  - Liu XY
AD  - Department of Pathology, West China Hospital, Sichuan University, Chengdu 610041, 
      China.
FAU - Chen, H J
AU  - Chen HJ
AD  - Department of Pathology, West China Hospital, Sichuan University, Chengdu 610041, 
      China.
FAU - Zhang, H Y
AU  - Zhang HY
AD  - Department of Pathology, West China Hospital, Sichuan University, Chengdu 610041, 
      China.
FAU - Bu, H
AU  - Bu H
AD  - Department of Pathology, West China Hospital, Sichuan University, Chengdu 610041, 
      China.
LA  - chi
GR  - 81972520/National Natural Science Foundation of China/
GR  - 2020YFS0270/Key R&D (Major Science and Technology Project) Project of Sichuan 
      Science and Technology Department/
GR  - 2018HXFH011/The 135 Project for Disciplines of Excellence-Clinical Research 
      Incubation Project, West China Hospital, Sichuan University/
PT  - Journal Article
PL  - China
TA  - Zhonghua Bing Li Xue Za Zhi
JT  - Zhonghua bing li xue za zhi = Chinese journal of pathology
JID - 0005331
RN  - 0 (Biomarkers, Tumor)
RN  - 63231-63-0 (RNA)
RN  - EC 2.3.2.27 (MDM2 protein, human)
RN  - EC 2.3.2.27 (Proto-Oncogene Proteins c-mdm2)
SB  - IM
MH  - Biomarkers, Tumor/genetics
MH  - Gene Amplification
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Liposarcoma/diagnosis/genetics
MH  - Proto-Oncogene Proteins c-mdm2/genetics
MH  - *RNA
EDAT- 2022/03/08 06:00
MHDA- 2022/03/09 06:00
CRDT- 2022/03/07 01:07
PHST- 2022/03/07 01:07 [entrez]
PHST- 2022/03/08 06:00 [pubmed]
PHST- 2022/03/09 06:00 [medline]
AID - 10.3760/cma.j.cn112151-20211029-00785 [doi]
PST - ppublish
SO  - Zhonghua Bing Li Xue Za Zhi. 2022 Mar 8;51(3):190-195. doi: 
      10.3760/cma.j.cn112151-20211029-00785.