PMID- 35249446
OWN - NLM
STAT- MEDLINE
DCOM- 20220408
LR  - 20220606
IS  - 2165-5987 (Electronic)
IS  - 2165-5979 (Print)
IS  - 2165-5979 (Linking)
VI  - 13
IP  - 3
DP  - 2022 Mar
TI  - Screening and preliminary identification of long non-coding RNAs critical for 
      osteogenic differentiation of human umbilical cord mesenchymal stem cells.
PG  - 6880-6894
LID - 10.1080/21655979.2022.2044274 [doi]
AB  - Human umbilical cord mesenchymal stem cells (hUCMSCs) are attractive therapeutic 
      cells for tissue engineering to treat bone defects. However, how the cells can 
      differentiate into bone remains unclear. Long non-coding RNAs (lncRNAs) are 
      non-coding RNAs that participate in many biological processes, including stem 
      cell differentiation. In this study, we investigated the profiles and functions 
      of lncRNAs in the osteogenic differentiation of hUCMSCs. We identified 343 
      lncRNAs differentially expressed during osteogenic differentiation, of which 115 
      were upregulated and 228 were downregulated. We further analyzed these lncRNAs 
      using bioinformatic analyses, including Gene Ontology (GO) and Kyoto Encyclopedia 
      of Genes and Genomes (KEGG) pathway analysis. GO and KEGG pathway analysis showed 
      that 'intracellular part' and 'Phosphatidylinositol signaling system' were the 
      most correlated molecular function and pathway, respectively. We selected the top 
      10 upregulated lncRNAs to construct six competing endogenous RNA networks. We 
      validated the impact of the lncRNA H19 on osteogenic differentiation by 
      overexpressing it in hUCMSCs. Overall, our results pave the way to detailed 
      studies of the molecular mechanisms of hUCMSC osteogenic differentiation, and 
      they provide a new theoretical basis to guide the therapeutic application of 
      hUCMSCs.
FAU - Zheng, Xiao
AU  - Zheng X
AD  - State Key Laboratory of Oral Diseases and National Clinical Research Center for 
      Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 
      Sichuan, China.
AD  - Department of Oral Prosthodontics, West China Hospital of Stomatology, Sichuan 
      University, Chengdu, Sichuan, China.
AD  - Department of Pediatric Dentistry, Shenzhen Stomatology Hospital (Pingshan), 
      Southern Medical University, Shenzhen, Guangdong, China.
FAU - Gan, Shuaiqi
AU  - Gan S
AD  - State Key Laboratory of Oral Diseases and National Clinical Research Center for 
      Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 
      Sichuan, China.
AD  - Department of Oral Prosthodontics, West China Hospital of Stomatology, Sichuan 
      University, Chengdu, Sichuan, China.
FAU - Su, Cheng
AU  - Su C
AD  - State Key Laboratory of Oral Diseases and National Clinical Research Center for 
      Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 
      Sichuan, China.
AD  - Department of Oral Prosthodontics, West China Hospital of Stomatology, Sichuan 
      University, Chengdu, Sichuan, China.
FAU - Zheng, Zheng
AU  - Zheng Z
AD  - State Key Laboratory of Oral Diseases and National Clinical Research Center for 
      Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 
      Sichuan, China.
AD  - Department of Oral Prosthodontics, West China Hospital of Stomatology, Sichuan 
      University, Chengdu, Sichuan, China.
FAU - Liao, Yihan
AU  - Liao Y
AD  - State Key Laboratory of Oral Diseases and National Clinical Research Center for 
      Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 
      Sichuan, China.
AD  - Department of Oral Prosthodontics, West China Hospital of Stomatology, Sichuan 
      University, Chengdu, Sichuan, China.
FAU - Shao, Jingjing
AU  - Shao J
AD  - State Key Laboratory of Oral Diseases and National Clinical Research Center for 
      Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 
      Sichuan, China.
AD  - Department of Oral Prosthodontics, West China Hospital of Stomatology, Sichuan 
      University, Chengdu, Sichuan, China.
FAU - Zhu, Zhimin
AU  - Zhu Z
AD  - State Key Laboratory of Oral Diseases and National Clinical Research Center for 
      Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 
      Sichuan, China.
AD  - Department of Oral Prosthodontics, West China Hospital of Stomatology, Sichuan 
      University, Chengdu, Sichuan, China.
FAU - Chen, Wenchuan
AU  - Chen W
AUID- ORCID: 0000-0002-6145-7878
AD  - State Key Laboratory of Oral Diseases and National Clinical Research Center for 
      Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 
      Sichuan, China.
AD  - Department of Oral Prosthodontics, West China Hospital of Stomatology, Sichuan 
      University, Chengdu, Sichuan, China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Bioengineered
JT  - Bioengineered
JID - 101581063
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Cell Differentiation/genetics
MH  - Gene Expression Profiling/methods
MH  - Humans
MH  - *Mesenchymal Stem Cells/metabolism
MH  - Osteogenesis/genetics
MH  - *RNA, Long Noncoding/genetics/metabolism
MH  - RNA, Messenger/genetics
MH  - Umbilical Cord/metabolism
PMC - PMC8973756
OTO - NOTNLM
OT  - Human umbilical cord mesenchymal stem cell
OT  - gene expression
OT  - long non-coding RNAs
OT  - osteogenic differentiation
COIS- No potential conflict of interest was reported by the author(s).
EDAT- 2022/03/08 06:00
MHDA- 2022/04/09 06:00
PMCR- 2022/03/07
CRDT- 2022/03/07 05:35
PHST- 2022/03/07 05:35 [entrez]
PHST- 2022/03/08 06:00 [pubmed]
PHST- 2022/04/09 06:00 [medline]
PHST- 2022/03/07 00:00 [pmc-release]
AID - 2044274 [pii]
AID - 10.1080/21655979.2022.2044274 [doi]
PST - ppublish
SO  - Bioengineered. 2022 Mar;13(3):6880-6894. doi: 10.1080/21655979.2022.2044274.