PMID- 35250640
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220309
IS  - 1664-042X (Print)
IS  - 1664-042X (Electronic)
IS  - 1664-042X (Linking)
VI  - 13
DP  - 2022
TI  - Sex-Specific Differences of Adenosine Triphosphate Levels in Red Blood Cells 
      Isolated From ApoE/LDLR Double-Deficient Mice.
PG  - 839323
LID - 10.3389/fphys.2022.839323 [doi]
LID - 839323
AB  - In this study for the first time, we investigated the correlation between 
      sex-specific differences in adenosine triphosphate (ATP) levels in red blood 
      cells (RBCs) and their mechanical, biochemical, and morphological alterations 
      during the progression of atherosclerosis in ApoE/LDLR double-deficient 
      (ApoE/LDLR(-/-)) mice. Our results indicate that both sex and age affect 
      alterations in RBCs of both ApoE/LDLR(-/-) and C57BL/6J mice. When compared with 
      male RBCs, female RBCs were characterized by lower basal ATP and mean corpuscular 
      hemoglobin concentration (MCHC), higher hemoglobin concentration (HGB), mean 
      corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), deformability, and 
      phosphatidylserine (PS) exposure levels, regardless of age in both, 
      ApoE/LDLR(-/-) and C57BL/6J mice. ApoE/LDLR(-/-) mice compared with age-matched 
      controls showed lower basal ATP levels regardless of age and sex. Intracellular 
      ATP level of RBCs was decreased solely in senescent female C57BL/6J mice, while 
      it was elevated in males. Basal extracellular ATP levels were 400 times lower 
      than corresponding intracellular level. In conclusion, basal ATP levels, RBC 
      morphology, deformability, PS exposure levels alterations are sex-dependent in 
      mice. Changes in basal ATP levels were correlated with PS exposure and trends of 
      changes in MCV. Trends of changes of the most RBC parameters were similar in both 
      sexes of ApoE/LDLR(-/-) mice compared with age-matched controls; however, their 
      kinetics and levels vary greatly between different stages of disease progression.
CI  - Copyright (c) 2022 Alcicek, Mohaissen, Bulat, Dybas, Szczesny-Malysiak, Kaczmarska, 
      Franczyk-Zarow, Kostogrys and Marzec.
FAU - Alcicek, Fatih Celal
AU  - Alcicek FC
AD  - Jagiellonian Centre for Experimental Therapeutics, Jagiellonian University, 
      Krakow, Poland.
FAU - Mohaissen, Tasnim
AU  - Mohaissen T
AD  - Jagiellonian Centre for Experimental Therapeutics, Jagiellonian University, 
      Krakow, Poland.
AD  - Chair and Faculty of Pharmacy, Jagiellonian University Medical College, Krakow, 
      Poland.
FAU - Bulat, Katarzyna
AU  - Bulat K
AD  - Jagiellonian Centre for Experimental Therapeutics, Jagiellonian University, 
      Krakow, Poland.
AD  - Lukasiewicz Research Network - Krakow Institute of Technology, Krakow, Poland.
FAU - Dybas, Jakub
AU  - Dybas J
AD  - Jagiellonian Centre for Experimental Therapeutics, Jagiellonian University, 
      Krakow, Poland.
FAU - Szczesny-Malysiak, Ewa
AU  - Szczesny-Malysiak E
AD  - Jagiellonian Centre for Experimental Therapeutics, Jagiellonian University, 
      Krakow, Poland.
FAU - Kaczmarska, Magdalena
AU  - Kaczmarska M
AD  - Jagiellonian Centre for Experimental Therapeutics, Jagiellonian University, 
      Krakow, Poland.
FAU - Franczyk-Zarow, Magdalena
AU  - Franczyk-Zarow M
AD  - Department of Human Nutrition and Dietetics, Faculty of Food Technology, 
      University of Agriculture, Krakow, Poland.
FAU - Kostogrys, Renata
AU  - Kostogrys R
AD  - Department of Human Nutrition and Dietetics, Faculty of Food Technology, 
      University of Agriculture, Krakow, Poland.
FAU - Marzec, Katarzyna M
AU  - Marzec KM
AD  - Jagiellonian Centre for Experimental Therapeutics, Jagiellonian University, 
      Krakow, Poland.
AD  - Lukasiewicz Research Network - Krakow Institute of Technology, Krakow, Poland.
LA  - eng
SI  - figshare/10.6084/m9.figshare.17008216.v1
PT  - Journal Article
DEP - 20220218
PL  - Switzerland
TA  - Front Physiol
JT  - Frontiers in physiology
JID - 101549006
PMC - PMC8895041
OTO - NOTNLM
OT  - ApoE/LDLR-/- mice
OT  - atherosclerosis
OT  - extracellular ATP
OT  - intracellular ATP
OT  - red blood cells
OT  - sex-related differences
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/03/08 06:00
MHDA- 2022/03/08 06:01
PMCR- 2022/02/18
CRDT- 2022/03/07 06:02
PHST- 2021/12/19 00:00 [received]
PHST- 2022/01/27 00:00 [accepted]
PHST- 2022/03/07 06:02 [entrez]
PHST- 2022/03/08 06:00 [pubmed]
PHST- 2022/03/08 06:01 [medline]
PHST- 2022/02/18 00:00 [pmc-release]
AID - 10.3389/fphys.2022.839323 [doi]
PST - epublish
SO  - Front Physiol. 2022 Feb 18;13:839323. doi: 10.3389/fphys.2022.839323. eCollection 
      2022.