PMID- 35251634
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220308
IS  - 2049-9469 (Electronic)
IS  - 2049-9450 (Print)
IS  - 2049-9450 (Linking)
VI  - 16
IP  - 4
DP  - 2022 Apr
TI  - Effects of endoplasmic reticulum stress-mediated CREB3L1 on apoptosis of glioma 
      cells.
PG  - 83
LID - 10.3892/mco.2022.2516 [doi]
LID - 83
AB  - The association between endoplasmic reticulum stress (ERS) and apoptosis has been 
      extensively studied. Cyclic adenosine monophosphate responsive element binding 
      protein 3 like 1 (CREB3L1) has an important role in the development of glioma. In 
      the present study, the potential association between ERS-induced apoptosis and 
      CREB3L1 and its clinical implications were investigated. From a total of 30 
      cases, brain gliomas with different pathological grades surgically resected at 
      the Department of Neurosurgery of the Affiliated Hospital of Guizhou Medical 
      University (Guiyang, China) between January 2018 and January 2019 were collected. 
      The expression of CREB3L1 and ERS-related proteins in gliomas with different 
      degrees of malignancy was detected by immunohistochemistry. Furthermore, U87-MG 
      glioma cells were cultured in vitro and treated with different concentrations of 
      ERS inducer thapsigargin (TG). The Cell Counting Kit-8 (CCK-8) assay was 
      performed to detect changes in cell activity at different incubation times and 
      drug concentrations. Cell apoptosis was detected by Annexin Ⅴ-FITC/propidium 
      iodide double staining and the protein expression levels of CREB3L1 and ERS were 
      detected by western blot analysis. Immunohistochemical analysis suggested that 
      the expression levels of CREB3L1, glucose-regulated protein, 78 kDa (GRP78) and 
      C/EBP-homologous protein (CHOP) in World Health Organization (WHO) grade I glioma 
      were higher than those in WHO grade Ⅱ-Ⅳ (all P<0.01). The results of the CCK-8 
      assay suggested that the activity of U87-MG glioma cells was significantly 
      decreased after treatment with TG (all P<0.05), and this effect was time- and 
      drug concentration-dependent. Flow cytometric analysis indicated that the 
      apoptotic rate of the cells was increased, which was significant when the 
      concentration of TG was 0.1 micromol/l (P<0.01). Furthermore, the protein expression 
      of CREB3L1, GRP78 and CHOP in glioma cells treated with TG was increased 
      (P<0.05). However, the expression level of Bcl-2 decreased (P<0.05). In 
      conclusion, ERS may reduce the cell proliferative activity and promote apoptosis 
      through mediating CREB3L1 expression. CREB3L1 may be a novel potential target for 
      glioma therapy.
CI  - Copyright (c) 2020, Spandidos Publications.
FAU - Yan, Zhao
AU  - Yan Z
AD  - Department of Clinical Medicine, Guizhou Medical University, Guiyang, Guizhou 
      550004, P.R. China.
AD  - Department of Neurosurgery, The Affiliated Hospital of Guizhou Medical 
      University, Guiyang, Guizhou 550001, P.R. China.
FAU - Hu, Yaxin
AU  - Hu Y
AD  - Department of Clinical Medicine, Guizhou Medical University, Guiyang, Guizhou 
      550004, P.R. China.
FAU - Zhang, Yiwei
AU  - Zhang Y
AD  - Department of Clinical Medicine, Guizhou Medical University, Guiyang, Guizhou 
      550004, P.R. China.
AD  - Department of Neurosurgery, The Affiliated Hospital of Guizhou Medical 
      University, Guiyang, Guizhou 550001, P.R. China.
FAU - Pu, Qian
AU  - Pu Q
AD  - Department of Clinical Medicine, Guizhou Medical University, Guiyang, Guizhou 
      550004, P.R. China.
FAU - Chu, Liangzhao
AU  - Chu L
AD  - Department of Clinical Medicine, Guizhou Medical University, Guiyang, Guizhou 
      550004, P.R. China.
AD  - Department of Neurosurgery, The Affiliated Hospital of Guizhou Medical 
      University, Guiyang, Guizhou 550001, P.R. China.
FAU - Liu, Jian
AU  - Liu J
AD  - Department of Clinical Medicine, Guizhou Medical University, Guiyang, Guizhou 
      550004, P.R. China.
AD  - Department of Neurosurgery, The Affiliated Hospital of Guizhou Medical 
      University, Guiyang, Guizhou 550001, P.R. China.
AD  - Department of Neurosurgery, Guizhou Provincial People's Hospital, Guiyang, 
      Guizhou 550002, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20220215
PL  - England
TA  - Mol Clin Oncol
JT  - Molecular and clinical oncology
JID - 101613422
PMC - PMC8892456
OTO - NOTNLM
OT  - CREB3L1
OT  - U87-MG
OT  - apoptosis
OT  - endoplasmic reticulum stress
OT  - glioma cells
OT  - thapsigargin
COIS- The authors declare that they have no competing interests.
EDAT- 2022/03/08 06:00
MHDA- 2022/03/08 06:01
PMCR- 2022/02/15
CRDT- 2022/03/07 06:05
PHST- 2021/10/20 00:00 [received]
PHST- 2022/01/24 00:00 [accepted]
PHST- 2022/03/07 06:05 [entrez]
PHST- 2022/03/08 06:00 [pubmed]
PHST- 2022/03/08 06:01 [medline]
PHST- 2022/02/15 00:00 [pmc-release]
AID - MCO-16-4-02516 [pii]
AID - 10.3892/mco.2022.2516 [doi]
PST - ppublish
SO  - Mol Clin Oncol. 2022 Apr;16(4):83. doi: 10.3892/mco.2022.2516. Epub 2022 Feb 15.