PMID- 35251768
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220308
IS  - 2162-2531 (Print)
IS  - 2162-2531 (Electronic)
IS  - 2162-2531 (Linking)
VI  - 27
DP  - 2022 Mar 8
TI  - Overexpression of cytosolic long noncoding RNA cytb protects against 
      pressure-overload-induced heart failure via sponging microRNA-103-3p.
PG  - 1127-1145
LID - 10.1016/j.omtn.2022.02.002 [doi]
AB  - Long noncoding RNAs (lncRNAs) play crucial roles in cardiovascular diseases. To 
      date, only limited studies have reported the role of mitochondria-derived lncRNAs 
      in heart failure (HF). In the current study, recombinant adeno-associated virus 9 
      was used to manipulate lncRNA cytb (lnccytb) expression in vivo. Fluorescence in 
      situ hybridization (FISH) assay was used to determine the location of lnccytb, 
      while microRNA (miRNA) sequencing and bioinformatics analyses were applied to 
      identify the downstream targets. The competitive endogenous RNA (ceRNA) function 
      of lnccytb was evaluated by biotin-coupled miRNA pull-down assays and luciferase 
      reporter assays. Results showed that lnccytb expression was decreased in the 
      heart of mice with transverse aortic constriction (TAC), as well as in the heart 
      and plasma of patients with HF. FISH assay and absolute RNA quantification via 
      real-time reverse transcription PCR suggested that the reduction of the lnccytb 
      transcripts mainly occurred in the cytosol. Upregulation of cytosolic lnccytb 
      attenuated cardiac dysfunction in TAC mice. Moreover, overexpression of cytosolic 
      lnccytb in cardiomyocytes alleviated isoprenaline-induced reactive oxidative 
      species (ROS) production and hypertrophy. Mechanistically, lnccytb acted as a 
      ceRNA via sponging miR-103-3p, ultimately mitigating the suppression of PTEN by 
      miR-103-3p. In summary, we demonstrated that the overexpression of cytosolic 
      lnccytb could ameliorate HF.
CI  - (c) 2022 The Author(s).
FAU - Zhang, Xudong
AU  - Zhang X
AD  - Division of Cardiology, Tongji Hospital, Tongji Medical College and Hubei Key 
      Laboratory of Genetics and Molecular Mechanisms of Cardiologic Disorders, 
      Huazhong University of Science and Technology, 1095# Jiefang Avenue, Wuhan 
      430030, China.
FAU - Yuan, Shuai
AU  - Yuan S
AD  - Division of Cardiology, Tongji Hospital, Tongji Medical College and Hubei Key 
      Laboratory of Genetics and Molecular Mechanisms of Cardiologic Disorders, 
      Huazhong University of Science and Technology, 1095# Jiefang Avenue, Wuhan 
      430030, China.
FAU - Liu, Jingbo
AU  - Liu J
AD  - Division of Cardiology, Tongji Hospital, Tongji Medical College and Hubei Key 
      Laboratory of Genetics and Molecular Mechanisms of Cardiologic Disorders, 
      Huazhong University of Science and Technology, 1095# Jiefang Avenue, Wuhan 
      430030, China.
FAU - Tang, Yuyan
AU  - Tang Y
AD  - Division of Cardiology, Tongji Hospital, Tongji Medical College and Hubei Key 
      Laboratory of Genetics and Molecular Mechanisms of Cardiologic Disorders, 
      Huazhong University of Science and Technology, 1095# Jiefang Avenue, Wuhan 
      430030, China.
FAU - Wang, Yan
AU  - Wang Y
AD  - Division of Cardiology, Tongji Hospital, Tongji Medical College and Hubei Key 
      Laboratory of Genetics and Molecular Mechanisms of Cardiologic Disorders, 
      Huazhong University of Science and Technology, 1095# Jiefang Avenue, Wuhan 
      430030, China.
FAU - Zhan, Jiabing
AU  - Zhan J
AD  - Division of Cardiology, Tongji Hospital, Tongji Medical College and Hubei Key 
      Laboratory of Genetics and Molecular Mechanisms of Cardiologic Disorders, 
      Huazhong University of Science and Technology, 1095# Jiefang Avenue, Wuhan 
      430030, China.
FAU - Fan, Jiahui
AU  - Fan J
AD  - Division of Cardiology, Tongji Hospital, Tongji Medical College and Hubei Key 
      Laboratory of Genetics and Molecular Mechanisms of Cardiologic Disorders, 
      Huazhong University of Science and Technology, 1095# Jiefang Avenue, Wuhan 
      430030, China.
FAU - Nie, Xiang
AU  - Nie X
AD  - Division of Cardiology, Tongji Hospital, Tongji Medical College and Hubei Key 
      Laboratory of Genetics and Molecular Mechanisms of Cardiologic Disorders, 
      Huazhong University of Science and Technology, 1095# Jiefang Avenue, Wuhan 
      430030, China.
FAU - Zhao, Yanru
AU  - Zhao Y
AD  - Division of Cardiology, Tongji Hospital, Tongji Medical College and Hubei Key 
      Laboratory of Genetics and Molecular Mechanisms of Cardiologic Disorders, 
      Huazhong University of Science and Technology, 1095# Jiefang Avenue, Wuhan 
      430030, China.
FAU - Wen, Zheng
AU  - Wen Z
AD  - Division of Cardiology, Tongji Hospital, Tongji Medical College and Hubei Key 
      Laboratory of Genetics and Molecular Mechanisms of Cardiologic Disorders, 
      Huazhong University of Science and Technology, 1095# Jiefang Avenue, Wuhan 
      430030, China.
FAU - Li, Huaping
AU  - Li H
AD  - Division of Cardiology, Tongji Hospital, Tongji Medical College and Hubei Key 
      Laboratory of Genetics and Molecular Mechanisms of Cardiologic Disorders, 
      Huazhong University of Science and Technology, 1095# Jiefang Avenue, Wuhan 
      430030, China.
FAU - Chen, Chen
AU  - Chen C
AD  - Division of Cardiology, Tongji Hospital, Tongji Medical College and Hubei Key 
      Laboratory of Genetics and Molecular Mechanisms of Cardiologic Disorders, 
      Huazhong University of Science and Technology, 1095# Jiefang Avenue, Wuhan 
      430030, China.
FAU - Wang, Dao Wen
AU  - Wang DW
AD  - Division of Cardiology, Tongji Hospital, Tongji Medical College and Hubei Key 
      Laboratory of Genetics and Molecular Mechanisms of Cardiologic Disorders, 
      Huazhong University of Science and Technology, 1095# Jiefang Avenue, Wuhan 
      430030, China.
LA  - eng
PT  - Journal Article
DEP - 20220210
PL  - United States
TA  - Mol Ther Nucleic Acids
JT  - Molecular therapy. Nucleic acids
JID - 101581621
PMC - PMC8881631
OTO - NOTNLM
OT  - GRSF1
OT  - PTEN
OT  - ceRNA
OT  - cytosol
OT  - heart failure
OT  - lncRNA
OT  - miR-103-3p
COIS- The authors declare no competing interests.
EDAT- 2022/03/08 06:00
MHDA- 2022/03/08 06:01
PMCR- 2022/02/10
CRDT- 2022/03/07 06:06
PHST- 2021/06/13 00:00 [received]
PHST- 2022/02/06 00:00 [accepted]
PHST- 2022/03/07 06:06 [entrez]
PHST- 2022/03/08 06:00 [pubmed]
PHST- 2022/03/08 06:01 [medline]
PHST- 2022/02/10 00:00 [pmc-release]
AID - S2162-2531(22)00030-0 [pii]
AID - 10.1016/j.omtn.2022.02.002 [doi]
PST - epublish
SO  - Mol Ther Nucleic Acids. 2022 Feb 10;27:1127-1145. doi: 
      10.1016/j.omtn.2022.02.002. eCollection 2022 Mar 8.