PMID- 35254478
OWN - NLM
STAT- MEDLINE
DCOM- 20220913
LR  - 20240426
IS  - 1432-0851 (Electronic)
IS  - 0340-7004 (Print)
IS  - 0340-7004 (Linking)
VI  - 71
IP  - 10
DP  - 2022 Oct
TI  - Immunosuppressive properties of human PD-1 + , PDL-1 + and CD80 + dendritic cells 
      from lymph nodes aspirates of lung cancer patients.
PG  - 2469-2483
LID - 10.1007/s00262-022-03178-5 [doi]
AB  - Dendritic cells (DCs) play a pivotal role in the homeostasis of the immune 
      system. The tumor microenvironment impairs the proper function of DCs. The 
      immunomodulatory properties of DCs in lung cancer are of interest. In the present 
      study, we analysed DCs subsets and immune cells with the expression of 
      immunomodulatory molecules: PD-1 and PD-L1 and co-stimulatory molecule CD80 in 
      metastatic, non-metastatic lymph nodes (LNs) and peripheral blood (PB). LNs 
      aspirates were obtained during the EBUS/TBNA procedure of 29 patients with 
      primary lung cancer. The cells were analyzed by flow cytometry. We reported a 
      higher percentage of DCs in the metastatic than in the non-metastatic LNs and the 
      PB (0.709% vs. 0.166% vs. 0.043%, p < 0.0001). The proportions of PD-1 + , 
      PD-L1 + and CD80 + DCs were higher in the metastatic LNs than in the 
      non-metastatic ones. A higher proportion of regulatory DCs (DCregs) was found in 
      the metastatic ones than in the non-metastatic LNs (22.5% vs. 3.1%, p = 0.0189). 
      We report that DCs cells show increased expression of PD-1, PD-L1 and CD80 
      molecules that can interact with T lymphocytes. It can be assumed that mature DCs 
      infiltrating metastatic LNs can develop into DCregs, which are involved in the 
      suppression of anti-tumor response.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, 
      part of Springer Nature.
FAU - Kwiecien, Iwona
AU  - Kwiecien I
AUID- ORCID: 0000-0003-2266-971X
AD  - Department of Internal Medicine and Hematology, Laboratory of Hematology and Flow 
      Cytometry, Military Institute of Medicine, Szaserow 128 Street, 04-141, Warsaw, 
      Poland. kwiecieniwi@gmail.com.
FAU - Rutkowska, Elzbieta
AU  - Rutkowska E
AD  - Department of Internal Medicine and Hematology, Laboratory of Hematology and Flow 
      Cytometry, Military Institute of Medicine, Szaserow 128 Street, 04-141, Warsaw, 
      Poland.
FAU - Raniszewska, Agata
AU  - Raniszewska A
AD  - Department of Internal Medicine and Hematology, Laboratory of Hematology and Flow 
      Cytometry, Military Institute of Medicine, Szaserow 128 Street, 04-141, Warsaw, 
      Poland.
FAU - Sokolowski, Rafal
AU  - Sokolowski R
AD  - Department of Internal Medicine, Pulmonology, Allergology and Clinical 
      Immunology, Military Institute of Medicine, Szaserow 128 Street, 04-141, Warsaw, 
      Poland.
FAU - Bednarek, Joanna
AU  - Bednarek J
AD  - Department of Internal Medicine, Pulmonology, Allergology and Clinical 
      Immunology, Military Institute of Medicine, Szaserow 128 Street, 04-141, Warsaw, 
      Poland.
FAU - Jahnz-Rozyk, Karina
AU  - Jahnz-Rozyk K
AD  - Department of Internal Medicine, Pulmonology, Allergology and Clinical 
      Immunology, Military Institute of Medicine, Szaserow 128 Street, 04-141, Warsaw, 
      Poland.
FAU - Rzepecki, Piotr
AU  - Rzepecki P
AD  - Department of Internal Medicine and Hematology, Military Institute of Medicine, 
      Szaserow 128 Street, 04-141, Warsaw, Poland.
FAU - Domagala-Kulawik, Joanna
AU  - Domagala-Kulawik J
AD  - Department of Internal Medicine, Pulmonary Diseases and Allergy, Medical 
      University of Warsaw, Banacha 1a Street, 02-097, Warsaw, Poland.
LA  - eng
GR  - 559/Military Institute of Medicine Warsaw/
PT  - Journal Article
DEP - 20220307
PL  - Germany
TA  - Cancer Immunol Immunother
JT  - Cancer immunology, immunotherapy : CII
JID - 8605732
RN  - 0 (B7-1 Antigen)
RN  - 0 (B7-H1 Antigen)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Programmed Cell Death 1 Receptor)
SB  - IM
MH  - B7-1 Antigen
MH  - *B7-H1 Antigen/metabolism
MH  - Cell Adhesion Molecules/metabolism
MH  - Dendritic Cells
MH  - Humans
MH  - *Lung Neoplasms/pathology
MH  - Lymph Nodes
MH  - Programmed Cell Death 1 Receptor
MH  - Tumor Microenvironment
PMC - PMC10991341
OTO - NOTNLM
OT  - CD80
OT  - Dendritic cells
OT  - Lung cancer
OT  - PD-1
OT  - PD-L1
OT  - Regulatory dendritic cells
COIS- The authors declare that they have no conflict of interest.
EDAT- 2022/03/08 06:00
MHDA- 2022/09/14 06:00
PMCR- 2022/03/07
CRDT- 2022/03/07 12:17
PHST- 2021/11/24 00:00 [received]
PHST- 2022/01/11 00:00 [accepted]
PHST- 2022/03/08 06:00 [pubmed]
PHST- 2022/09/14 06:00 [medline]
PHST- 2022/03/07 12:17 [entrez]
PHST- 2022/03/07 00:00 [pmc-release]
AID - 10.1007/s00262-022-03178-5 [pii]
AID - 3178 [pii]
AID - 10.1007/s00262-022-03178-5 [doi]
PST - ppublish
SO  - Cancer Immunol Immunother. 2022 Oct;71(10):2469-2483. doi: 
      10.1007/s00262-022-03178-5. Epub 2022 Mar 7.