PMID- 35255248
OWN - NLM
STAT- Publisher
LR  - 20231013
IS  - 1090-2414 (Electronic)
IS  - 0147-6513 (Linking)
VI  - 234
DP  - 2022 Apr 1
TI  - A high-throughput assay for screening the abilities of per- and polyfluoroalkyl 
      substances in inducing plasma kallikrein-like activity.
PG  - 113381
LID - S0147-6513(22)00221-4 [pii]
LID - 10.1016/j.ecoenv.2022.113381 [doi]
AB  - The plasma consists of multiple functional serine zymogens, such as plasma 
      kallikrein-kinin system (KKS), which are vulnerable to exogenous chemical 
      exposure, and may closely relate to the deleterious effects. Testing whether the 
      anthropogenic chemicals could increase the kallikrein-like activity in plasma or 
      not would be of great help to understand their potentials in triggering the 
      cascade activation of the plasma zymogens and explain the corresponding 
      hematotoxicity. In this study, a novel high-throughput ex vivo assay was 
      established to screen the abilities of emerging chemicals like per- and 
      polyfluoroalkyl substances (PFASs) in inducing kallikrein-like activities on 
      basis of using rat plasma as the protease zymogen source. Upon the optimization 
      of the conditions in the test system, the assay gave sensitive fluorescent 
      response to the stimulation of the positive control, dextran sulfate, and the 
      dose-response showed a typical S-shaped curve with EC(50) of 0.24 mg/L. The 
      intra-plate and inter-plate relative standard deviations (RSDs) were less than 
      10% in the quantitative range of dextran sulfate, indicating a good reliability 
      and repeatability of this newly-established assay. Using this method, several 
      alternatives or congeners of perfluorooctanesulfonic acid (PFOS) and 
      perfluorooctanoic acid (PFOA), including 6:2 chlorinated polyfluoroalkyl ether 
      sulfonate (6:2 Cl-PFESA), Ag-PFOA, K-PFOA, Na-PFOA and ammonium 
      pentadecafluorooctanoate (APFO), were further screened, and their capabilities in 
      inducing kallikrein-like activities were identified. The ex vivo assay 
      newly-developed in the present study would be promising in high-throughput 
      screening of the hematological effects of emerging chemicals of concern.
CI  - Copyright (c) 2022 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Liu, Qian S
AU  - Liu QS
AD  - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research 
      Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 
      100085, China.
FAU - Zhang, Yuzhu
AU  - Zhang Y
AD  - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research 
      Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 
      100085, China; College of Resources and Environment, University of Chinese 
      Academy of Sciences, Beijing 100049, China.
FAU - Sun, Zhendong
AU  - Sun Z
AD  - School of Environment, Hangzhou Institute for Advanced Study, University of 
      Chinese Academy of Sciences, Hangzhou 310000, China.
FAU - Gao, Yurou
AU  - Gao Y
AD  - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research 
      Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 
      100085, China; Sino-Danish College, University of Chinese Academy of Sciences, 
      Beijing 100049, China.
FAU - Zhou, Qunfang
AU  - Zhou Q
AD  - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research 
      Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 
      100085, China; College of Resources and Environment, University of Chinese 
      Academy of Sciences, Beijing 100049, China; Institute of Environment and Health, 
      Jianghan University, Wuhan 430056, China. Electronic address: zhouqf@rcees.ac.cn.
FAU - Jiang, Guibin
AU  - Jiang G
AD  - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research 
      Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 
      100085, China; College of Resources and Environment, University of Chinese 
      Academy of Sciences, Beijing 100049, China.
LA  - eng
PT  - Journal Article
DEP - 20220304
PL  - Netherlands
TA  - Ecotoxicol Environ Saf
JT  - Ecotoxicology and environmental safety
JID - 7805381
SB  - IM
OTO - NOTNLM
OT  - Ex-vivo assay
OT  - Hematological effects
OT  - High-throughput screening
OT  - Kallikrein-like activity
OT  - Per- and polyfluoroalkyl substances
EDAT- 2022/03/08 06:00
MHDA- 2022/03/08 06:00
CRDT- 2022/03/07 20:08
PHST- 2021/10/24 00:00 [received]
PHST- 2022/02/23 00:00 [revised]
PHST- 2022/03/01 00:00 [accepted]
PHST- 2022/03/08 06:00 [pubmed]
PHST- 2022/03/08 06:00 [medline]
PHST- 2022/03/07 20:08 [entrez]
AID - S0147-6513(22)00221-4 [pii]
AID - 10.1016/j.ecoenv.2022.113381 [doi]
PST - ppublish
SO  - Ecotoxicol Environ Saf. 2022 Apr 1;234:113381. doi: 10.1016/j.ecoenv.2022.113381. 
      Epub 2022 Mar 4.