PMID- 35257983
OWN - NLM
STAT- MEDLINE
DCOM- 20220405
LR  - 20220405
IS  - 1873-264X (Electronic)
IS  - 0731-7085 (Linking)
VI  - 213
DP  - 2022 May 10
TI  - Development of a rapid and sensitive LC-MS/MS assay for the quanti fi cation of 
      commonly abused drugs in Asia in a micro-segment of a single hair using 
      microwave-assisted extraction and dansyl chloride derivatization.
PG  - 114678
LID - S0731-7085(22)00099-1 [pii]
LID - 10.1016/j.jpba.2022.114678 [doi]
AB  - A high-throughput method using microwave-assisted extraction, chemical 
      derivatization and liquid chromatography-electrospray tandem mass spectrometry 
      (LC-ESI-MS/MS) was developed for the simultaneous analysis of illicit drugs and 
      their metabolites, including amphetamine (AMP), methamphetamine (MA), 
      methylenedioxy- amphetamine (MDA), methylenedioxy-methamphetamine (MDMA), 
      morphine (MOR), 6-acetylmorphine (6-AM), ketamine (K), and norketamine (NK) in a 
      micro-segment of a single hair (0.4 mm). In order to elevate the throughput and 
      sensitivities of selected compounds, 3 min microwave-assisted extraction and 
      10 min derivatization with dansyl chloride (DC) were employed. After 
      derivatization, all compounds except ketamine and norketamine were derivatized 
      and enhanced the sensitivities significantly. Derivatives showed intense fragment 
      ions and low background noise on DC-MOR, DC-6-AM, and four DC-amphetamine-type 
      stimulants. The total sample preparation and analysis time was 50 min. The 
      calibration range was from LOQ to 5000 pg/mg, the coefficient of determination 
      was better than 0.997. Intra-assay precision and accuracy were generally less 
      than 15%. Limits of detection ranged from 15 to 50 pg/mg, limits of quantitation 
      ranged between 45 and 125 pg/mg. The matrix effect was better than 90%. The 
      method was successfully applied to the analysis of actual hair samples collected 
      from multi-drug abusers. This advanced method showed practicality in hair 
      analysis and was suitable for the extremely insufficient sample.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Chen, Hsiu-Chuan
AU  - Chen HC
AD  - Institute of Medicine, Chung Shan Medical University, Taichung 40201, Taiwan.
FAU - Lee, Pin-Duo
AU  - Lee PD
AD  - Institute of Medicine, Chung Shan Medical University, Taichung 40201, Taiwan.
FAU - Chang, Yan-Zin
AU  - Chang YZ
AD  - Institute of Medicine, Chung Shan Medical University, Taichung 40201, Taiwan; 
      Drug Testing Center, Department of Clinical Laboratory, Chung Shan Medical 
      University Hospital, Taichung 40201, Taiwan. Electronic address: yzc@csmu.edu.tw.
LA  - eng
PT  - Journal Article
DEP - 20220218
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 0 (Dansyl Compounds)
RN  - 0 (Illicit Drugs)
RN  - CK833KGX7E (Amphetamine)
RN  - QMU9166TJ4 (dansyl chloride)
SB  - IM
MH  - Amphetamine
MH  - Chromatography, Liquid
MH  - Dansyl Compounds
MH  - Hair/chemistry
MH  - *Illicit Drugs/analysis
MH  - Microwaves
MH  - Substance Abuse Detection/methods
MH  - *Tandem Mass Spectrometry/methods
OTO - NOTNLM
OT  - Dansyl chloride
OT  - Drugs of abuse
OT  - Hair
OT  - LC-MS/MS
OT  - Segmental analysis
EDAT- 2022/03/09 06:00
MHDA- 2022/04/06 06:00
CRDT- 2022/03/08 08:45
PHST- 2021/11/24 00:00 [received]
PHST- 2022/02/15 00:00 [revised]
PHST- 2022/02/16 00:00 [accepted]
PHST- 2022/03/09 06:00 [pubmed]
PHST- 2022/04/06 06:00 [medline]
PHST- 2022/03/08 08:45 [entrez]
AID - S0731-7085(22)00099-1 [pii]
AID - 10.1016/j.jpba.2022.114678 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2022 May 10;213:114678. doi: 10.1016/j.jpba.2022.114678. 
      Epub 2022 Feb 18.