PMID- 35259598
OWN - NLM
STAT- MEDLINE
DCOM- 20220406
LR  - 20220406
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 267
DP  - 2022 Apr
TI  - Inhibition of the antigen-presenting ability of dendritic cells by non-structural 
      protein 2 of influenza A virus.
PG  - 109392
LID - S0378-1135(22)00062-1 [pii]
LID - 10.1016/j.vetmic.2022.109392 [doi]
AB  - Influenza A viruses (IAV), including human IAV and avian IAV (H9N2 subtype), are 
      recurring of influenza outbreaks worldwide in a wide range of mammalian and avian 
      species. Dendritic cells (DCs) are specialised antigen presenting cells. Although 
      DCs can take up IAV and transmit it to other cells, it still unclear why DCs do 
      not effectively present IAV antigens. In this study, we found that Non-structural 
      protein 2 (NS2) of IAV inhibited the maturation and antigen-presenting ability of 
      DCs. We then examined a potential involvement of microRNAs (miRNAs). Analyses of 
      avian DCs stimulated with avian IAV identified 9 upregulated and 10 downregulated 
      miRNAs. However, nearly none microRNA has been significantly altered by NS2 
      stimulation. Moreover, we found that NS2 binds to exportin 5 (Xpo5), which 
      inhibited miRNA biogenesis. Thus, hijacking of the miRNA biogenesis pathway 
      appears to be one mechanism by which NS2 impairs antigen presentation. 
      Furthermore, we found that NS2 directly interacts with interferon regulatory 
      factor 3, which also inhibits the antigen-presenting ability of DCs. These 
      results thus indicate that NS2-mediated impairment of antigen presentation by DCs 
      might be a mechanism that contributes to the prevalence of the influenza virus.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Lin, Jian
AU  - Lin J
AD  - College of Veterinary medicine, Nanjing Agricultural University, Wei gang 1, 
      Nanjing, Jiangsu 210095, PR China; College of Life Sciences, Nanjing Agricultural 
      University, Wei gang 1, Nanjing, Jiangsu 210095, PR China. Electronic address: 
      linjian@njau.edu.cn.
FAU - Cao, Yanan
AU  - Cao Y
AD  - College of Veterinary medicine, Nanjing Agricultural University, Wei gang 1, 
      Nanjing, Jiangsu 210095, PR China.
FAU - Shah, Abid Ullah
AU  - Shah AU
AD  - College of Veterinary medicine, Nanjing Agricultural University, Wei gang 1, 
      Nanjing, Jiangsu 210095, PR China.
FAU - Zuo, Jinjiao
AU  - Zuo J
AD  - College of Life Sciences, Nanjing Agricultural University, Wei gang 1, Nanjing, 
      Jiangsu 210095, PR China.
FAU - Zhang, Shuai
AU  - Zhang S
AD  - College of Veterinary medicine, Nanjing Agricultural University, Wei gang 1, 
      Nanjing, Jiangsu 210095, PR China.
FAU - Yu, Qinghua
AU  - Yu Q
AD  - College of Veterinary medicine, Nanjing Agricultural University, Wei gang 1, 
      Nanjing, Jiangsu 210095, PR China.
FAU - Chong, Mark M W
AU  - Chong MMW
AD  - St Vincent's Institute of Medical Research, Fitzroy, Victoria, Australia; 
      Department of Medicine (St Vincent's), University of Melbourne, Fitzroy, 
      Victoria, Australia.
FAU - Yang, Qian
AU  - Yang Q
AD  - College of Veterinary medicine, Nanjing Agricultural University, Wei gang 1, 
      Nanjing, Jiangsu 210095, PR China. Electronic address: zxbyq@njau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20220304
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Animals
MH  - Antigen Presentation
MH  - Dendritic Cells
MH  - Humans
MH  - *Influenza A Virus, H9N2 Subtype/genetics
MH  - *Influenza, Human
MH  - Mammals
MH  - *MicroRNAs/genetics/metabolism
OTO - NOTNLM
OT  - Antigen presentation
OT  - Dendritic cells
OT  - Influenza A virus
OT  - MicroRNA
OT  - Non-structural protein
OT  - Xpo5
EDAT- 2022/03/09 06:00
MHDA- 2022/04/07 06:00
CRDT- 2022/03/08 20:11
PHST- 2021/12/20 00:00 [received]
PHST- 2022/02/23 00:00 [revised]
PHST- 2022/02/27 00:00 [accepted]
PHST- 2022/03/09 06:00 [pubmed]
PHST- 2022/04/07 06:00 [medline]
PHST- 2022/03/08 20:11 [entrez]
AID - S0378-1135(22)00062-1 [pii]
AID - 10.1016/j.vetmic.2022.109392 [doi]
PST - ppublish
SO  - Vet Microbiol. 2022 Apr;267:109392. doi: 10.1016/j.vetmic.2022.109392. Epub 2022 
      Mar 4.