PMID- 35261819
OWN - NLM
STAT- MEDLINE
DCOM- 20230110
LR  - 20230111
IS  - 2167-8359 (Print)
IS  - 2167-8359 (Electronic)
IS  - 2167-8359 (Linking)
VI  - 10
DP  - 2022
TI  - LncRNA CCAT1 facilitates the proliferation, invasion and migration of human 
      laryngeal squamous cell carcinoma cells via the miR-218-5p/BMI1.
PG  - e12961
LID - 10.7717/peerj.12961 [doi]
LID - e12961
AB  - Long non-coding RNAs (LncRNAs) are vital in the treatment of laryngeal squamous 
      cell carcinoma (LSCC). This study estimated the mechanism of lncRNA CCAT1 (CCAT1) 
      in LSCC cells. The expression of CCAT1 in the human laryngeal mucosal epithelial 
      cells (HLCs) and LSCC cells (Hep-2 and TU177) was detected. CCK-8 and Transwell 
      assays were used to evaluate the cell proliferative, migrative, and invasive 
      abilities, respectively. The subcellular localization of CCAT1 was verified by 
      RNA-FISH and cytoplasmic isolation assays. The targeted relationship among CCAT1, 
      miR-218-5p, and BMI1 was verified by dual-luciferase assay. Expressions of 
      miR-218-5p and BMI1 were detected by RT-qPCR. Our results depicted that CCAT1 was 
      highly-expressed in Hep-2 and TU177 cells. Silencing CCAT1 inhibited the 
      proliferation, migration, and invasion of Hep-2 and TU177 cells. Mechanically, 
      CCAT1 regulated the BMI1 expression by competitively binding to miR-218-5p as a 
      competing endogenous RNA (ceRNA), and thus facilitated the growth of Hep-2 and 
      TU177 cells. Downregulation of miR-218-5p or upregulation of BMI1 inhibited the 
      inhibitory effect of silencing CCAT1 on Hep-2 and TU177 cell proliferation, 
      invasion, and migration. In conclusion, our study elicited that lncRNA CCAT1 
      facilitated the proliferation, migration, and invasion of Hep-2 and TU177 cells 
      by sponging miR-218-5p and regulating the downstream BMI1.
CI  - (c) 2022 Hong et al.
FAU - Hong, Jing
AU  - Hong J
AD  - Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, 
      Nanchang, China.
FAU - Hong, Ali
AU  - Hong A
AD  - Jiangxi University of Traditional Chinese Medicine, Nanchang, China.
FAU - Tu, Houshu
AU  - Tu H
AD  - Nanchang Angel Maternity Hospital, Nanchang, China.
FAU - Wan, Zhichao
AU  - Wan Z
AD  - Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, 
      Nanchang, China.
FAU - Deng, Yuqiao
AU  - Deng Y
AD  - Jiangxi University of Traditional Chinese Medicine, Nanchang, China.
FAU - Deng, Chengcheng
AU  - Deng C
AD  - Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, 
      Nanchang, China.
FAU - Tao, Bo
AU  - Tao B
AD  - Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, 
      Nanchang, China.
FAU - Yu, Yanjin
AU  - Yu Y
AD  - Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, 
      Nanchang, China.
FAU - Zhou, Lanfei
AU  - Zhou L
AD  - Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, 
      Nanchang, China.
LA  - eng
PT  - Journal Article
DEP - 20220303
PL  - United States
TA  - PeerJ
JT  - PeerJ
JID - 101603425
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (MicroRNAs)
RN  - 0 (BMI1 protein, human)
RN  - EC 2.3.2.27 (Polycomb Repressive Complex 1)
RN  - 0 (MIRN218 microRNA, human)
MH  - Humans
MH  - *RNA, Long Noncoding/genetics
MH  - Squamous Cell Carcinoma of Head and Neck/genetics
MH  - *MicroRNAs/genetics
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - *Head and Neck Neoplasms
MH  - Polycomb Repressive Complex 1/genetics
PMC - PMC8898548
OTO - NOTNLM
OT  - BMI1
OT  - Competing endogenous RNA
OT  - Invasion
OT  - Laryngeal squamous cell carcinoma
OT  - LncRNA CCAT1
OT  - Migration
OT  - Proliferation
OT  - miR-218-5p
COIS- The authors declare that they have no competing interests.
EDAT- 2022/03/10 06:00
MHDA- 2022/03/10 06:01
PMCR- 2022/03/03
CRDT- 2022/03/09 08:44
PHST- 2021/09/16 00:00 [received]
PHST- 2022/01/27 00:00 [accepted]
PHST- 2022/03/09 08:44 [entrez]
PHST- 2022/03/10 06:00 [pubmed]
PHST- 2022/03/10 06:01 [medline]
PHST- 2022/03/03 00:00 [pmc-release]
AID - 12961 [pii]
AID - 10.7717/peerj.12961 [doi]
PST - epublish
SO  - PeerJ. 2022 Mar 3;10:e12961. doi: 10.7717/peerj.12961. eCollection 2022.