PMID- 35262507
OWN - NLM
STAT- Publisher
LR  - 20240220
IS  - 0236-6290 (Print)
IS  - 0236-6290 (Linking)
DP  - 2022 Mar 9
TI  - Paraquat modulates immunological function in bone marrow-derived macrophages.
LID - 10.1556/004.2022.00003 [doi]
AB  - The herbicide paraquat (PQ) is known to affect the immune system. Many reports 
      have indicated that PQ impacts on the viability and functions of the immune 
      cells, however, the underlying mechanism in detail is still unknown. The aim of 
      this study was to evaluate the effects of PQ on the free radical production, 
      oxidative stress, cell death and pro-inflammatory gene expression of murine bone 
      marrow-derived macrophages (BMDMs) from C57BL/6NJcl mice in vitro. BMDMs were 
      incubated with PQ at 0, 200 and 400 microM concentrations for 24 h. Intracellular 
      reactive oxygen species (ROS) production, apoptosis, cell viability, nitric 
      oxide, inducible nitric oxide synthase (iNOS), and IL-6 expression levels were 
      measured. The results revealed that PQ treatments led to a decrease in the cell 
      viability and induced apoptotic cell death in a dose-dependent manner. 
      Additionally, PQ also induced the generation of ROS. The mRNA level of the 
      pro-inflammatory mediator genes iNOS and IL-6 were also elevated, while the level 
      of lipid peroxide (malondialdehyde) production remained unaltered. Interestingly, 
      the PQ treatment led to a decrease in the nitric oxide production. These results 
      indicate that the increased cellular ROS production, due to the PQ treatment, 
      induces apoptosis and the herbicide triggers production of iNOS and IL-6 in 
      BMDMs.
FAU - Srinontong, Piyarat
AU  - Srinontong P
AUID- ORCID: 0000-0001-6391-4203
AD  - 1 Faculty of Veterinary Sciences, Mahasarakham University, Maha Sarakham, 
      Thailand.
AD  - 2 Bioveterinary Research Unit, Faculty of Veterinary Sciences, Mahasarakham 
      University, Maha Sarakham, Thailand.
FAU - Wandee, Jaroon
AU  - Wandee J
AD  - 1 Faculty of Veterinary Sciences, Mahasarakham University, Maha Sarakham, 
      Thailand.
AD  - 2 Bioveterinary Research Unit, Faculty of Veterinary Sciences, Mahasarakham 
      University, Maha Sarakham, Thailand.
FAU - Aengwanich, Worapol
AU  - Aengwanich W
AD  - 1 Faculty of Veterinary Sciences, Mahasarakham University, Maha Sarakham, 
      Thailand.
AD  - 3 Stress and Oxidative Stress in Animal Research Unit, Faculty of Veterinary 
      Sciences, Mahasarakham University, Maha Sarakham, Thailand.
LA  - eng
PT  - Journal Article
DEP - 20220309
PL  - Hungary
TA  - Acta Vet Hung
JT  - Acta veterinaria Hungarica
JID - 8406376
SB  - IM
OTO - NOTNLM
OT  - apoptosis
OT  - bone marrow-derived macrophages
OT  - paraquat
OT  - pro-inflammatory mediator
OT  - reactive oxygen species
EDAT- 2022/03/10 06:00
MHDA- 2022/03/10 06:00
CRDT- 2022/03/09 12:32
PHST- 2021/05/10 00:00 [received]
PHST- 2022/01/31 00:00 [accepted]
PHST- 2022/03/09 12:32 [entrez]
PHST- 2022/03/10 06:00 [pubmed]
PHST- 2022/03/10 06:00 [medline]
AID - 10.1556/004.2022.00003 [doi]
PST - aheadofprint
SO  - Acta Vet Hung. 2022 Mar 9. doi: 10.1556/004.2022.00003.