PMID- 35265860
OWN - NLM
STAT- MEDLINE
DCOM- 20220422
LR  - 20220422
IS  - 2666-1667 (Electronic)
IS  - 2666-1667 (Linking)
VI  - 3
IP  - 1
DP  - 2022 Mar 18
TI  - A DNA-fiber protocol for single molecule analysis of telomere (SMAT) length and 
      extension events in cancer cells.
PG  - 101212
LID - 10.1016/j.xpro.2022.101212 [doi]
LID - 101212
AB  - Alternative lengthening of telomeres (ALT) is a homologous recombination-based 
      telomere maintenance mechanism. It is active in approximately 10-15% of cancers. 
      We present a DNA-fiber protocol, combining YOYO-1 staining of genomic DNA, 
      telomere fluorescence in situ hybridization (FISH), and EdU labeling of nascent 
      DNA, to measure telomere extension events in ALT cancer cells. The protocol can 
      be used to delineate ALT-mediated telomere extension. For complete details on the 
      use and execution of this protocol, please refer to Barroso-Gonzalez et al. 
      (2021).
CI  - (c) 2022 The Author(s).
FAU - Lu, Robert
AU  - Lu R
AD  - Children's Medical Research Institute, University of Sydney, Westmead, NSW 2145, 
      Australia.
FAU - Allen, Joshua A M
AU  - Allen JAM
AD  - Children's Medical Research Institute, University of Sydney, Westmead, NSW 2145, 
      Australia.
FAU - Galaviz, Pablo
AU  - Galaviz P
AD  - Children's Medical Research Institute, University of Sydney, Westmead, NSW 2145, 
      Australia.
FAU - Pickett, Hilda A
AU  - Pickett HA
AD  - Children's Medical Research Institute, University of Sydney, Westmead, NSW 2145, 
      Australia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220304
PL  - United States
TA  - STAR Protoc
JT  - STAR protocols
JID - 101769501
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - DNA/genetics
MH  - In Situ Hybridization, Fluorescence/methods
MH  - *Neoplasms/genetics
MH  - *Single Molecule Imaging
MH  - Telomere/genetics
MH  - Telomere Homeostasis/genetics
PMC - PMC8899046
OTO - NOTNLM
OT  - Cancer
OT  - Cell Biology
OT  - In Situ Hybridization
OT  - Microscopy
OT  - Molecular Biology
COIS- The authors declare no competing interests.
EDAT- 2022/03/11 06:00
MHDA- 2022/04/23 06:00
PMCR- 2022/03/04
CRDT- 2022/03/10 05:47
PHST- 2022/03/10 05:47 [entrez]
PHST- 2022/03/11 06:00 [pubmed]
PHST- 2022/04/23 06:00 [medline]
PHST- 2022/03/04 00:00 [pmc-release]
AID - S2666-1667(22)00092-2 [pii]
AID - 101212 [pii]
AID - 10.1016/j.xpro.2022.101212 [doi]
PST - epublish
SO  - STAR Protoc. 2022 Mar 4;3(1):101212. doi: 10.1016/j.xpro.2022.101212. eCollection 
      2022 Mar 18.