PMID- 35266323
OWN - NLM
STAT- MEDLINE
DCOM- 20220426
LR  - 20220716
IS  - 2324-9269 (Electronic)
IS  - 2324-9269 (Linking)
VI  - 10
IP  - 5
DP  - 2022 May
TI  - CircFOXM1 acts as a ceRNA to upregulate SMAD2 and promote the progression of 
      nasopharyngeal carcinoma.
PG  - e1914
LID - 10.1002/mgg3.1914 [doi]
LID - e1914
AB  - BACKGROUND: In recent years, the development of high-throughput sequencing 
      technology has promoted the rapid development of circRNA-related research. 
      Studies have found that circRNA plays a key role in a variety of tumors, but few 
      people study the role of circRNA in nasopharyngeal carcinoma. Under comprehensive 
      treatments, the 5-year survival rate can reach about 70%, but some patients still 
      have distant metastases or recurrences after treatment. Therefore, it is very 
      important to study the molecular mechanisms of the proliferation and invasion of 
      nasopharyngeal carcinoma. METHODS: QRT-PCR was applied to detect the relative 
      expression level of circFOXM1 in NPC and nasopharyngeal epithelial cell lines. We 
      knocked down circFOXM1 and studied the influence of circFOXM1 on NPC cells. 
      Nuclear and cytoplasmic RNA isolation experiments, fluorescence in situ 
      hybridization (FISH), bioinformatics analysis, the dual-luciferase reporter 
      experiment, Western Blot, and other experiments were conducted to verify the 
      relationships among circFOXM1, miR-136-5p, and SMAD2. We collected clinical NPC 
      samples to prove the effect of circFOXM1 on the prognosis and treatment of NPC. 
      RESULTS: In this study, we found that circFOXM1 is highly expressed in 
      nasopharyngeal carcinoma tissue cells compared with adjacent normal tissues and 
      is related to the staging of nasopharyngeal carcinoma. High expression of 
      circFOXM1 indicates a poor prognosis for nasopharyngeal carcinoma. Knockdown of 
      CircFOXM1 inhibited the proliferation and invasion of nasopharyngeal carcinoma 
      cells. CONCLUSION: CircFOXM1 promotes the malignant proliferation of 
      nasopharyngeal carcinoma cells by regulating the miR-136-5p-SMAD2 axis.
CI  - (c) 2022 The Authors. Molecular Genetics & Genomic Medicine published by Wiley 
      Periodicals LLC.
FAU - Pei, Shuai
AU  - Pei S
AUID- ORCID: 0000-0001-8523-3484
AD  - Xuzhou Medical University, Xuzhou, Jiangsu, China.
AD  - Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, Nanjing Medical 
      University Affiliated Cancer Hospital, Nanjing, Jiangsu, China.
FAU - Ma, Chengxian
AU  - Ma C
AD  - Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, Nanjing Medical 
      University Affiliated Cancer Hospital, Nanjing, Jiangsu, China.
FAU - Chen, Jie
AU  - Chen J
AD  - Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, Nanjing Medical 
      University Affiliated Cancer Hospital, Nanjing, Jiangsu, China.
FAU - Hu, Xinyu
AU  - Hu X
AD  - Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, Nanjing Medical 
      University Affiliated Cancer Hospital, Nanjing, Jiangsu, China.
FAU - Du, Mingyu
AU  - Du M
AD  - Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, Nanjing Medical 
      University Affiliated Cancer Hospital, Nanjing, Jiangsu, China.
FAU - Xu, Tian
AU  - Xu T
AD  - Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, Nanjing Medical 
      University Affiliated Cancer Hospital, Nanjing, Jiangsu, China.
FAU - Zhan, Mengna
AU  - Zhan M
AD  - Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, Nanjing Medical 
      University Affiliated Cancer Hospital, Nanjing, Jiangsu, China.
FAU - Xue, Ke
AU  - Xue K
AD  - Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, Nanjing Medical 
      University Affiliated Cancer Hospital, Nanjing, Jiangsu, China.
FAU - Zhang, Yufeng
AU  - Zhang Y
AD  - Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, Nanjing Medical 
      University Affiliated Cancer Hospital, Nanjing, Jiangsu, China.
FAU - Yin, Li
AU  - Yin L
AD  - Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, Nanjing Medical 
      University Affiliated Cancer Hospital, Nanjing, Jiangsu, China.
FAU - He, Xia
AU  - He X
AD  - Xuzhou Medical University, Xuzhou, Jiangsu, China.
AD  - Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, Nanjing Medical 
      University Affiliated Cancer Hospital, Nanjing, Jiangsu, China.
LA  - eng
PT  - Journal Article
DEP - 20220310
PL  - United States
TA  - Mol Genet Genomic Med
JT  - Molecular genetics & genomic medicine
JID - 101603758
RN  - 0 (FOXM1 protein, human)
RN  - 0 (Forkhead Box Protein M1)
RN  - 0 (MIRN136 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
RN  - 0 (SMAD2 protein, human)
RN  - 0 (Smad2 Protein)
SB  - IM
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Forkhead Box Protein M1
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *MicroRNAs/genetics
MH  - *Nasopharyngeal Carcinoma/genetics
MH  - *Nasopharyngeal Neoplasms/genetics
MH  - *RNA, Circular
MH  - Smad2 Protein/genetics/metabolism
PMC - PMC9034685
OTO - NOTNLM
OT  - NPC
OT  - ceRNA
OT  - circFOXM1
OT  - invasion
OT  - miR-136-5p
OT  - migration
COIS- The authors declare that they have no conflict of interest in this work.
EDAT- 2022/03/11 06:00
MHDA- 2022/04/27 06:00
PMCR- 2022/03/10
CRDT- 2022/03/10 05:51
PHST- 2021/08/04 00:00 [revised]
PHST- 2020/12/30 00:00 [received]
PHST- 2022/02/17 00:00 [accepted]
PHST- 2022/03/11 06:00 [pubmed]
PHST- 2022/04/27 06:00 [medline]
PHST- 2022/03/10 05:51 [entrez]
PHST- 2022/03/10 00:00 [pmc-release]
AID - MGG31914 [pii]
AID - 10.1002/mgg3.1914 [doi]
PST - ppublish
SO  - Mol Genet Genomic Med. 2022 May;10(5):e1914. doi: 10.1002/mgg3.1914. Epub 2022 
      Mar 10.