PMID- 35267680
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220316
IS  - 2073-4360 (Electronic)
IS  - 2073-4360 (Linking)
VI  - 14
IP  - 5
DP  - 2022 Feb 22
TI  - 3D Printed Scaffold Based on Type I Collagen/PLGA_TGF-beta1 Nanoparticles Mimicking 
      the Growth Factor Footprint of Human Bone Tissue.
LID - 10.3390/polym14050857 [doi]
LID - 857
AB  - In bone regenerative strategies, the controlled release of growth factors is one 
      of the main aspects for successful tissue regeneration. Recent trends in the drug 
      delivery field increased the interest in the development of biodegradable systems 
      able to protect and transport active agents. In the present study, we designed 
      degradable poly(lactic-co-glycolic)acid (PLGA) nanocarriers suitable for the 
      release of Transforming Growth Factor-beta 1 (TGF-beta1), a key molecule in the 
      management of bone cells behaviour. Spherical TGF-beta1-containing PLGA 
      (PLGA_TGF-beta1) nanoparticles (ca.250 nm) exhibiting high encapsulation efficiency 
      (ca.64%) were successfully synthesized. The TGF-beta1 nanocarriers were subsequently 
      combined with type I collagen for the fabrication of nanostructured 3D printed 
      scaffolds able to mimic the TGF-beta1 presence in the human bone extracellular 
      matrix (ECM). The homogeneous hybrid formulation underwent a comprehensive 
      rheological characterisation in view of 3D printing. The 3D printed 
      collagen-based scaffolds (10 mm x 10 mm x 1 mm) successfully mimicked the TGF-beta1 
      presence in human bone ECM as assessed by immunohistochemical TGF-beta1 staining, 
      covering ca.3.4% of the whole scaffold area. Moreover, the collagenous matrix was 
      able to reduce the initial burst release observed in the first 24 h from about 
      38% for the PLGA_TGF-beta1 alone to 14.5%, proving that the nanocarriers 
      incorporation into collagen allows achieving sustained release kinetics.
FAU - Banche-Niclot, Federica
AU  - Banche-Niclot F
AUID- ORCID: 0000-0002-5855-4490
AD  - Department of Applied Science and Technology, Politecnico di Torino, 10129 
      Torino, Italy.
FAU - Licini, Caterina
AU  - Licini C
AUID- ORCID: 0000-0002-0018-8096
AD  - Department of Clinical and Molecular Sciences (DISCLIMO), Universita Politecnica 
      delle Marche, 60126 Ancona, Italy.
FAU - Montalbano, Giorgia
AU  - Montalbano G
AUID- ORCID: 0000-0003-2348-2949
AD  - Department of Applied Science and Technology, Politecnico di Torino, 10129 
      Torino, Italy.
FAU - Fiorilli, Sonia
AU  - Fiorilli S
AD  - Department of Applied Science and Technology, Politecnico di Torino, 10129 
      Torino, Italy.
AD  - National Interuniversity Consortium of Materials Science and Technology, RU 
      Politecnico di Torino, 50121 Firenze, Italy.
FAU - Mattioli-Belmonte, Monica
AU  - Mattioli-Belmonte M
AUID- ORCID: 0000-0002-2087-2776
AD  - Department of Clinical and Molecular Sciences (DISCLIMO), Universita Politecnica 
      delle Marche, 60126 Ancona, Italy.
FAU - Vitale-Brovarone, Chiara
AU  - Vitale-Brovarone C
AD  - Department of Applied Science and Technology, Politecnico di Torino, 10129 
      Torino, Italy.
AD  - National Interuniversity Consortium of Materials Science and Technology, RU 
      Politecnico di Torino, 50121 Firenze, Italy.
LA  - eng
GR  - 681798-BOOST/ERC_/European Research Council/International
GR  - R16L8T5F8T-GRACE/Italian Minister of Education, Universities and Research (MIUR), 
      Progetto FARE Ricerca in Italia/
PT  - Journal Article
DEP - 20220222
PL  - Switzerland
TA  - Polymers (Basel)
JT  - Polymers
JID - 101545357
PMC - PMC8912467
OTO - NOTNLM
OT  - 3D printed scaffolds
OT  - TGF-beta1
OT  - bone
OT  - drug delivery
OT  - polymeric nanoparticles
OT  - tissue regeneration
OT  - type I collagen
COIS- The authors declare no conflict of interest.
EDAT- 2022/03/11 06:00
MHDA- 2022/03/11 06:01
PMCR- 2022/02/22
CRDT- 2022/03/10 15:25
PHST- 2022/01/28 00:00 [received]
PHST- 2022/02/11 00:00 [revised]
PHST- 2022/02/18 00:00 [accepted]
PHST- 2022/03/10 15:25 [entrez]
PHST- 2022/03/11 06:00 [pubmed]
PHST- 2022/03/11 06:01 [medline]
PHST- 2022/02/22 00:00 [pmc-release]
AID - polym14050857 [pii]
AID - polymers-14-00857 [pii]
AID - 10.3390/polym14050857 [doi]
PST - epublish
SO  - Polymers (Basel). 2022 Feb 22;14(5):857. doi: 10.3390/polym14050857.