PMID- 35268220
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230308
IS  - 2076-2615 (Print)
IS  - 2076-2615 (Electronic)
IS  - 2076-2615 (Linking)
VI  - 12
IP  - 5
DP  - 2022 Mar 4
TI  - Sperm Gone Smart: A Portable Device (iSperm((R))) to Assess Semen Concentration and 
      Motility in Dogs.
LID - 10.3390/ani12050652 [doi]
LID - 652
AB  - The iSperm(R) is a portable device for semen analysis. This study aimed to 
      investigate its correlation with a conventional computer-assisted sperm analyzer 
      (ISAS(R)v1) for the assessment of semen concentration and kinematic parameters in 
      dogs (n = 224). The intra-assay variability of both devices and their ability to 
      estimate semen concentration at a fixed value of 40 x 106/mL were also 
      investigated. Results showed that the intra-assay variability was lower for the 
      ISAS(R)v1 for all parameters compared to the iSperm(R). Hence, iSperm(R) estimates were 
      more variable in-between fields. Both the iSperm(R) and the ISAS(R)v1 were not 
      reliable in estimating semen concentration (ISAS(R)v1: median 30 x 106/mL, 
      interquartile range (IQR) 12, p < 0.01; iSperm(R): median 35.12 x 106/mL, IQR 
      11.11, p < 0.01). Finally, positive correlations were found between both devices 
      with stronger correlations obtained when four fields were analyzed by the 
      iSperm(R). However, the low number of spermatozoa analyzed per field and the 
      inability to avoid artifacts are downsides that currently limit the reliability 
      of the iSperm(R). Therefore, the software of iSperm(R) needs some improvement to make 
      it a valid and practical alternative to automated computerized systems for the 
      analysis of canine semen.
FAU - Domain, Guillaume
AU  - Domain G
AUID- ORCID: 0000-0002-6017-9263
AD  - Department of Internal Medicine, Reproduction and Population Medicine, Faculty of 
      Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, 
      Belgium.
FAU - Banchi, Penelope
AU  - Banchi P
AUID- ORCID: 0000-0001-6061-4889
AD  - Department of Internal Medicine, Reproduction and Population Medicine, Faculty of 
      Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, 
      Belgium.
AD  - Department of Veterinary Sciences, University of Turin, Largo Paolo Braccini 2-5, 
      10095 Grugliasco, Italy.
FAU - Ali Hassan, Hiba
AU  - Ali Hassan H
AD  - Department of Internal Medicine, Reproduction and Population Medicine, Faculty of 
      Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, 
      Belgium.
FAU - Eilers, Anouk
AU  - Eilers A
AD  - Department of Internal Medicine, Reproduction and Population Medicine, Faculty of 
      Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, 
      Belgium.
FAU - Lannoo, Joke
AU  - Lannoo J
AD  - Department of Internal Medicine, Reproduction and Population Medicine, Faculty of 
      Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, 
      Belgium.
FAU - Wydooghe, Eline
AU  - Wydooghe E
AD  - Department of Internal Medicine, Reproduction and Population Medicine, Faculty of 
      Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, 
      Belgium.
FAU - Nizanski, Wojciech
AU  - Nizanski W
AUID- ORCID: 0000-0003-1056-0784
AD  - Department of Reproduction and Clinic of Farm Animals, University of 
      Environmental Science, Grundwaldzki Square 49, 50-357 Wroclaw, Poland.
FAU - Van Soom, Ann
AU  - Van Soom A
AUID- ORCID: 0000-0001-5010-6311
AD  - Department of Internal Medicine, Reproduction and Population Medicine, Faculty of 
      Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, 
      Belgium.
LA  - eng
GR  - 3s038019/Research Foundation - Flanders/
GR  - PPI/APM/2019/1/00044/U/00001/Polish National Agency for Academic Exchange/
PT  - Journal Article
DEP - 20220304
PL  - Switzerland
TA  - Animals (Basel)
JT  - Animals : an open access journal from MDPI
JID - 101635614
PMC - PMC8909599
OTO - NOTNLM
OT  - automated device
OT  - canine
OT  - semen analysis
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript, or in the decision to publish the results.
EDAT- 2022/03/11 06:00
MHDA- 2022/03/11 06:01
PMCR- 2022/03/04
CRDT- 2022/03/10 15:28
PHST- 2022/01/31 00:00 [received]
PHST- 2022/02/25 00:00 [revised]
PHST- 2022/03/01 00:00 [accepted]
PHST- 2022/03/10 15:28 [entrez]
PHST- 2022/03/11 06:00 [pubmed]
PHST- 2022/03/11 06:01 [medline]
PHST- 2022/03/04 00:00 [pmc-release]
AID - ani12050652 [pii]
AID - animals-12-00652 [pii]
AID - 10.3390/ani12050652 [doi]
PST - epublish
SO  - Animals (Basel). 2022 Mar 4;12(5):652. doi: 10.3390/ani12050652.