PMID- 35269382
OWN - NLM
STAT- MEDLINE
DCOM- 20220411
LR  - 20220411
IS  - 2073-4409 (Electronic)
IS  - 2073-4409 (Linking)
VI  - 11
IP  - 5
DP  - 2022 Feb 22
TI  - Cerebral Organoids Maintain the Expression of Neural Stem Cell-Associated 
      Glycoepitopes and Extracellular Matrix.
LID - 10.3390/cells11050760 [doi]
LID - 760
AB  - During development, the nervous system with its highly specialized cell types 
      forms from a pool of relatively uniform stem cells. This orchestrated process 
      requires tight regulation. The extracellular matrix (ECM) is a complex network 
      rich in signaling molecules, and therefore, of interest in this context. Distinct 
      carbohydrate structures, bound to ECM molecules like Tenascin C (TNC), are 
      associated with neural stem/progenitor cells. We have analyzed the expression 
      patterns of the LewisX (LeX) trisaccharide motif and of the sulfation-dependent 
      DSD-1 chondroitin sulfate glycosaminoglycan epitope in human cerebral organoids, 
      a 3D model for early central nervous system (CNS) development, 
      immunohistochemically. In early organoids we observed distinct expression 
      patterns of the glycoepitopes, associated with rosette-like structures that 
      resemble the neural tube in vitro: Terminal LeX motifs, recognized by the 
      monoclonal antibody (mAb) 487(LeX), were enriched in the lumen and at the outer 
      border of neural rosettes. In contrast, internal LeX motif repeats detected with 
      mAb 5750(LeX) were concentrated near the lumen. The DSD-1 epitope, labeled with 
      mAb 473HD, was detectable at rosette borders and in adjacent cells. The epitope 
      expression was maintained in older organoids but appeared more diffuse. The 
      differential glycoepitope expression suggests a specific function in the 
      developing human CNS.
FAU - Roll, Lars
AU  - Roll L
AD  - Department of Cell Morphology and Molecular Neurobiology, Ruhr University Bochum, 
      44780 Bochum, Germany.
FAU - Lessmann, Katrin
AU  - Lessmann K
AD  - Department of Cell Morphology and Molecular Neurobiology, Ruhr University Bochum, 
      44780 Bochum, Germany.
FAU - Brustle, Oliver
AU  - Brustle O
AD  - Institute of Reconstructive Neurobiology, Medical Faculty & University Hospital 
      Bonn, University of Bonn, 53127 Bonn, Germany.
FAU - Faissner, Andreas
AU  - Faissner A
AUID- ORCID: 0000-0002-2211-8259
AD  - Department of Cell Morphology and Molecular Neurobiology, Ruhr University Bochum, 
      44780 Bochum, Germany.
LA  - eng
GR  - Fa 159/23-1/Deutsche Forschungsgemeinschaft/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220222
PL  - Switzerland
TA  - Cells
JT  - Cells
JID - 101600052
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Epitopes)
RN  - 0 (Tenascin)
SB  - IM
MH  - Aged
MH  - Antibodies, Monoclonal/metabolism
MH  - Epitopes
MH  - Extracellular Matrix/metabolism
MH  - Humans
MH  - *Neural Stem Cells/metabolism
MH  - *Organoids/metabolism
MH  - Tenascin/metabolism
PMC - PMC8909158
OTO - NOTNLM
OT  - DSD-1 epitope
OT  - LewisX epitope
OT  - PTPRZ1
OT  - Tenascin C
OT  - cerebral organoid
OT  - chondroitin sulfate
OT  - extracellular matrix
OT  - glycoepitope
OT  - neural stem cell
OT  - radial glia
COIS- The funders had no role in the design of the study; in the collection, analyses, 
      or interpretation of data; in the writing of the manuscript, or in the decision 
      to publish the results.
EDAT- 2022/03/11 06:00
MHDA- 2022/04/12 06:00
PMCR- 2022/02/22
CRDT- 2022/03/10 15:34
PHST- 2021/12/23 00:00 [received]
PHST- 2022/02/10 00:00 [revised]
PHST- 2022/02/14 00:00 [accepted]
PHST- 2022/03/10 15:34 [entrez]
PHST- 2022/03/11 06:00 [pubmed]
PHST- 2022/04/12 06:00 [medline]
PHST- 2022/02/22 00:00 [pmc-release]
AID - cells11050760 [pii]
AID - cells-11-00760 [pii]
AID - 10.3390/cells11050760 [doi]
PST - epublish
SO  - Cells. 2022 Feb 22;11(5):760. doi: 10.3390/cells11050760.