PMID- 35269816 OWN - NLM STAT- MEDLINE DCOM- 20220408 LR - 20220408 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 23 IP - 5 DP - 2022 Feb 28 TI - Identification and DNA Marker Development for a Wheat-Leymus mollis 2Ns (2D) Disomic Chromosome Substitution. LID - 10.3390/ijms23052676 [doi] LID - 2676 AB - Leymus mollis (2n = 4x = 28, NsNsXmXm), a wild relative of common wheat (Triticum aestivum L.), carries numerous loci which could potentially be used in wheat improvement. In this study, line 17DM48 was isolated from the progeny of a wheat and L. mollis hybrid. This line has 42 chromosomes forming 21 bivalents at meiotic metaphase I. Genomic in situ hybridization (GISH) demonstrated the presence of a pair chromosomes from the Ns genome of L. mollis. This pair substituted for wheat chromosome 2D, as shown by fluorescence in situ hybridization (FISH), DNA marker analysis, and hybridization to wheat 55K SNP array. Therefore, 17DM48 is a wheat-L. mollis 2Ns (2D) disomic substitution line. It shows longer spike and a high level of stripe rust resistance. Using specific-locus amplified fragment sequencing (SLAF-seq), 13 DNA markers were developed to identify and trace chromosome 2Ns of L. mollis in wheat background. This line provides a potential bridge germplasm for genetic improvement of wheat stripe rust resistance. FAU - Feng, Xianbo AU - Feng X AD - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Agronomy, Northwest A&F University, Yangling, Xianyang 712100, China. AD - Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling, Xianyang 712100, China. FAU - Du, Xin AU - Du X AD - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Agronomy, Northwest A&F University, Yangling, Xianyang 712100, China. AD - Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling, Xianyang 712100, China. FAU - Wang, Siwen AU - Wang S AD - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Agronomy, Northwest A&F University, Yangling, Xianyang 712100, China. AD - Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling, Xianyang 712100, China. FAU - Deng, Pingchuan AU - Deng P AD - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Agronomy, Northwest A&F University, Yangling, Xianyang 712100, China. AD - Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling, Xianyang 712100, China. FAU - Wang, Yongfu AU - Wang Y AD - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Agronomy, Northwest A&F University, Yangling, Xianyang 712100, China. AD - Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling, Xianyang 712100, China. FAU - Shang, Lihui AU - Shang L AD - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Agronomy, Northwest A&F University, Yangling, Xianyang 712100, China. AD - Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling, Xianyang 712100, China. FAU - Tian, Zengrong AU - Tian Z AD - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Agronomy, Northwest A&F University, Yangling, Xianyang 712100, China. AD - Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling, Xianyang 712100, China. FAU - Wang, Changyou AU - Wang C AD - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Agronomy, Northwest A&F University, Yangling, Xianyang 712100, China. AD - Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling, Xianyang 712100, China. FAU - Chen, Chunhuan AU - Chen C AD - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Agronomy, Northwest A&F University, Yangling, Xianyang 712100, China. AD - Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling, Xianyang 712100, China. FAU - Zhao, Jixin AU - Zhao J AD - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Agronomy, Northwest A&F University, Yangling, Xianyang 712100, China. AD - Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling, Xianyang 712100, China. FAU - Ji, Wanquan AU - Ji W AUID- ORCID: 0000-0003-0663-4568 AD - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Agronomy, Northwest A&F University, Yangling, Xianyang 712100, China. AD - Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling, Xianyang 712100, China. LA - eng GR - 4,500,000 RMB Yuan/Shaanxi Scientific Observation and Experiment Station of Crop Gene Resources and Germplasm Innovation Program/ PT - Journal Article DEP - 20220228 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Genetic Markers) SB - IM MH - *Basidiomycota/genetics MH - Chromosomes, Plant/genetics MH - Disease Resistance/genetics MH - Genetic Markers MH - In Situ Hybridization, Fluorescence MH - Plant Diseases/genetics MH - Poaceae/genetics MH - *Triticum/genetics PMC - PMC8911044 OTO - NOTNLM OT - Leymus mollis OT - SLAF-seq OT - molecular cytogenetics OT - stripe rust OT - wheat 55K array COIS- The authors declare no conflict of interest. EDAT- 2022/03/11 06:00 MHDA- 2022/04/09 06:00 PMCR- 2022/02/28 CRDT- 2022/03/10 15:36 PHST- 2021/12/24 00:00 [received] PHST- 2022/02/17 00:00 [revised] PHST- 2022/02/27 00:00 [accepted] PHST- 2022/03/10 15:36 [entrez] PHST- 2022/03/11 06:00 [pubmed] PHST- 2022/04/09 06:00 [medline] PHST- 2022/02/28 00:00 [pmc-release] AID - ijms23052676 [pii] AID - ijms-23-02676 [pii] AID - 10.3390/ijms23052676 [doi] PST - epublish SO - Int J Mol Sci. 2022 Feb 28;23(5):2676. doi: 10.3390/ijms23052676.