PMID- 35269978
OWN - NLM
STAT- MEDLINE
DCOM- 20220408
LR  - 20220408
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 23
IP  - 5
DP  - 2022 Mar 4
TI  - Wound-Microenvironment Engineering through Advanced-Dressing Bioprinting.
LID - 10.3390/ijms23052836 [doi]
LID - 2836
AB  - In patients with comorbidities, a large number of wounds become chronic, 
      representing an overwhelming economic burden for healthcare systems. Engineering 
      the microenvironment is a paramount trend to activate cells and burst-healing 
      mechanisms. The extrusion bioprinting of advanced dressings was performed with 
      novel composite bioinks made by blending adipose decellularized extracellular 
      matrix with plasma and human dermal fibroblasts. Rheological and microstructural 
      assessments of the composite hydrogels supported post-printing cell viability and 
      proliferation over time. Embedded fibroblasts expressed steady concentrations of 
      extracellular matrix proteins, including type 1, 3 and 4 collagens and 
      fibronectin. ELISA assessments, multiplex protein arrays and ensuing 
      bioinformatic analyses revealed paracrine activities corresponding to 
      wound-healing activation through the modulation of inflammation and angiogenesis. 
      The two modalities of advanced dressings, differing in platelet number, showed 
      differences in the release of inflammatory and angiogenic cytokines, including 
      interleukin 8 (IL-8), monocyte chemotactic protein 1 (MCP-1), vascular 
      endothelial growth factor (VEGF) and hepatocyte growth factor (HGF). The 
      conditioned media stimulated human-dermal-cell proliferation over time. Our 
      findings open the door to engineering the microenvironment as a strategy to 
      enhance healing.
FAU - Del Amo, Cristina
AU  - Del Amo C
AUID- ORCID: 0000-0002-3796-4482
AD  - Regenerative Therapies, Bioprinting Laboratory, Biocruces Bizkaia Health Research 
      Institute, Cruces University Hospital, 48903 Barakaldo, Spain.
FAU - Fernandez-San Argimiro, Xabier
AU  - Fernandez-San Argimiro X
AD  - TECNALIA, Basque Research and Technology Alliance (BRTA), 20009 Donostia-San 
      Sebastian, Spain.
FAU - Cascajo-Castresana, Maria
AU  - Cascajo-Castresana M
AD  - TECNALIA, Basque Research and Technology Alliance (BRTA), 20009 Donostia-San 
      Sebastian, Spain.
FAU - Perez-Valle, Arantza
AU  - Perez-Valle A
AUID- ORCID: 0000-0002-3108-4978
AD  - Regenerative Therapies, Bioprinting Laboratory, Biocruces Bizkaia Health Research 
      Institute, Cruces University Hospital, 48903 Barakaldo, Spain.
FAU - Madarieta, Iratxe
AU  - Madarieta I
AUID- ORCID: 0000-0002-1097-9326
AD  - TECNALIA, Basque Research and Technology Alliance (BRTA), 20009 Donostia-San 
      Sebastian, Spain.
FAU - Olalde, Beatriz
AU  - Olalde B
AUID- ORCID: 0000-0003-4523-0849
AD  - TECNALIA, Basque Research and Technology Alliance (BRTA), 20009 Donostia-San 
      Sebastian, Spain.
FAU - Andia, Isabel
AU  - Andia I
AUID- ORCID: 0000-0003-0607-4767
AD  - Regenerative Therapies, Bioprinting Laboratory, Biocruces Bizkaia Health Research 
      Institute, Cruces University Hospital, 48903 Barakaldo, Spain.
LA  - eng
GR  - Program Elkartek B4H KK-2019-0006-BC/Basque Government/
PT  - Journal Article
DEP - 20220304
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Bandages
MH  - *Bioprinting
MH  - Extracellular Matrix/metabolism
MH  - Humans
MH  - Printing, Three-Dimensional
MH  - Tissue Engineering
MH  - Tissue Scaffolds/chemistry
MH  - Vascular Endothelial Growth Factor A/metabolism
PMC - PMC8911091
OTO - NOTNLM
OT  - 3D bioprinting
OT  - bioink
OT  - cytokines
OT  - decellularized adipose extracellular matrix
OT  - growth factors
OT  - plasma
OT  - platelet
OT  - wound healing
COIS- The authors declare no conflict of interest.
EDAT- 2022/03/11 06:00
MHDA- 2022/04/09 06:00
PMCR- 2022/03/04
CRDT- 2022/03/10 15:37
PHST- 2022/02/09 00:00 [received]
PHST- 2022/02/23 00:00 [revised]
PHST- 2022/03/02 00:00 [accepted]
PHST- 2022/03/10 15:37 [entrez]
PHST- 2022/03/11 06:00 [pubmed]
PHST- 2022/04/09 06:00 [medline]
PHST- 2022/03/04 00:00 [pmc-release]
AID - ijms23052836 [pii]
AID - ijms-23-02836 [pii]
AID - 10.3390/ijms23052836 [doi]
PST - epublish
SO  - Int J Mol Sci. 2022 Mar 4;23(5):2836. doi: 10.3390/ijms23052836.