PMID- 35271985
OWN - NLM
STAT- MEDLINE
DCOM- 20220414
LR  - 20220428
IS  - 1096-1208 (Electronic)
IS  - 0882-4010 (Linking)
VI  - 165
DP  - 2022 Apr
TI  - Genetic characterization and pathogenicity of a novel recombinant PRRSV from 
      lineage 1, 8 and 3 in China failed to infect MARC-145 cells.
PG  - 105469
LID - S0882-4010(22)00082-1 [pii]
LID - 10.1016/j.micpath.2022.105469 [doi]
AB  - The diversity of porcine reproductive and respiratory syndrome virus (PRRSV) in 
      China is increasing rapidly along with mutation and recombination. Recombination 
      could occur between inter- and intra-lineage of PRRSV, which accelerated the 
      complexity of pathogenicity and cell tropism of the recombinant strain. In the 
      present study, a novel PRRSV strain named HN-YL1711 was isolated from a pig farm 
      suffering from severe respiratory difficulty in Henan province, China. The whole 
      genomic sequence analysis indicated that the genome of HN-YL1711 was 15018 nt. It 
      shared 86%, 87.3%, 88.1%, 91.1%, 84.2%, and 84.1% nucleotide similarities with 
      PRRSVs VR2332, CH1a, JXA1, NADC30, QYYZ, and GM2, respectively. Based on 
      phylogenetic analysis of Nsp2, ORF5 and complete genomes, HN-YL1711 was 
      classified into lineage 1 of PRRSV. However, seven genomic break points were 
      detected in recombination analysis, which indicated that the HN-YL1711 originated 
      from multiple recombination among NADC30-like (major parent, lineage 1), 
      JXA1-like (minor parent, lineage 8), and QYYZ-like (minor parent, lineage 3) 
      PRRSV. Porcine alveolar macrophages (PAMs), 3D4/21-CD163 and MARC-145 cells were 
      used to explore the viral adaptation of HN-YL1711. The results indicated that it 
      could infect the PAMs but failed to infect MARC-145 cells. Challenge experiments 
      showed that HN-YL1711 exhibits intermediate virulence in pigs, compared with 
      HP-PRRSV JXA1 and LP-PRRSV CH1a. Taken together, our findings suggest that 
      recombination remains an important factor in PRRSV evolution and that 
      recombination further complicates the cell tropism and pathogenicity of PRRSV.
CI  - Copyright (c) 2022 Elsevier Ltd. All rights reserved.
FAU - Li, Yang
AU  - Li Y
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 
      China.
FAU - Jiao, Dian
AU  - Jiao D
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 
      China.
FAU - Jing, Yang
AU  - Jing Y
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 
      China.
FAU - He, Yuan
AU  - He Y
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 
      China.
FAU - Han, Weiguo
AU  - Han W
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 
      China.
FAU - Li, Zhiwei
AU  - Li Z
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 
      China.
FAU - Ma, Zhiqian
AU  - Ma Z
AD  - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, 
      China.
FAU - Feng, Yingtong
AU  - Feng Y
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 
      China.
FAU - Xiao, Shuqi
AU  - Xiao S
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 
      China. Electronic address: xiaoshuqi@nwsuaf.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20220307
PL  - England
TA  - Microb Pathog
JT  - Microbial pathogenesis
JID - 8606191
SB  - IM
EIN - Microb Pathog. 2022 Jun;167:105547. PMID: 35484058
MH  - Animals
MH  - China
MH  - Genetic Variation
MH  - Genome, Viral
MH  - Phylogeny
MH  - *Porcine Reproductive and Respiratory Syndrome
MH  - *Porcine respiratory and reproductive syndrome virus/genetics
MH  - Recombination, Genetic
MH  - Swine
MH  - Virulence/genetics
OTO - NOTNLM
OT  - Cell tropism
OT  - NADC30-like
OT  - Pathogenicity
OT  - Porcine reproductive and respiratory syndrome virus (PRRSV)
OT  - Recombination
EDAT- 2022/03/11 06:00
MHDA- 2022/04/15 06:00
CRDT- 2022/03/10 20:12
PHST- 2021/12/24 00:00 [received]
PHST- 2022/02/20 00:00 [revised]
PHST- 2022/03/06 00:00 [accepted]
PHST- 2022/03/11 06:00 [pubmed]
PHST- 2022/04/15 06:00 [medline]
PHST- 2022/03/10 20:12 [entrez]
AID - S0882-4010(22)00082-1 [pii]
AID - 10.1016/j.micpath.2022.105469 [doi]
PST - ppublish
SO  - Microb Pathog. 2022 Apr;165:105469. doi: 10.1016/j.micpath.2022.105469. Epub 2022 
      Mar 7.