PMID- 35272172
OWN - NLM
STAT- MEDLINE
DCOM- 20220510
LR  - 20220510
IS  - 1878-1705 (Electronic)
IS  - 1567-5769 (Linking)
VI  - 107
DP  - 2022 Jun
TI  - Characterization of immune-related genes andimmune infiltration features for 
      early diagnosis, prognosis and recognition of immunosuppression in sepsis.
PG  - 108650
LID - S1567-5769(22)00134-5 [pii]
LID - 10.1016/j.intimp.2022.108650 [doi]
AB  - Among the body systems, the immune system plays a fundamental role in the 
      pathophysiology of sepsis. The effects of immunogenomic and immune cell 
      infiltration in sepsis were still not been systematically understood. Based on 
      modified Lasso penalized regression and RF, 8 DEIRGs (ADM, CX3CR1, DEFA4, 
      HLA-DPA1, MAPK14, ORM1, RETN, and SLPI) were combined to construct an IRG 
      classifier. In the discovery cohort, IRG classifier exhibited superior diagnostic 
      efficacy and performed better in predicting mortality than clinical 
      characteristics or MARS/SRS endotypes. Encouragingly, similar results were 
      observed in the ArrayExpress databases. The use of hydrocortisone in IRG 
      high-risk subgroup was associated with increased risk of mortality. In IRG 
      low-risk phenotypes, NK cells, T helper cells, and infiltrating lymphocyte (IL) 
      are significantly richer, while T cells regulatory (Tregs) and myeloid-derived 
      suppressor cells (MDSC) are more abundant in IRG high-risk phenotypes. IRG score 
      were significantly negatively correlated with Cytokine cytokine receptor 
      interaction (CCR) and human leukocyte antigen (HLA). Between the IRG subgroups, 
      the expression levels of several cytokines (IL-10, IFNG, TNF) were significantly 
      different, and IRG score was significantly positively correlated with ratio of 
      IL-10/TNF. Results of qRT-PCR validated that higher expression level of ADM, 
      DEFA4, MAPK14, ORM1, RETN, and SLPI as well as lower expression level of CX3CR1 
      and HLA-DPA1 in sepsis samples compared to control sample. A diagnostic and 
      prognostic model, namely IRG classifier, was established based on 8 IRGs that is 
      closely correlated with responses to hydrocortisone and immunosuppression status 
      and might facilitate personalized counseling for specific therapy.
CI  - Copyright (c) 2022. Published by Elsevier B.V.
FAU - Lu, Jianhai
AU  - Lu J
AD  - Department of Intensive Care Unit, Shunde Hospital, Southern Medical University 
      (The First People's Hospital of Shunde), Foshan 528308, Guangdong Province, PR 
      China.
FAU - Chen, Rui
AU  - Chen R
AD  - Department of Medical Intensive Care Unit, General Hospital of Southern Theater 
      Command, PLA, Guangzhou 510010, Guangdong Province, PR China.
FAU - Ou, Yangpeng
AU  - Ou Y
AD  - Department of Oncology, Huizhou Third People's Hospital, Guangzhou Medical 
      University, Huizhou, 516000, Guangdong Province, PR China.
FAU - Jiang, Qianhua
AU  - Jiang Q
AD  - Department of Intensive Care Unit, Shunde Hospital, Southern Medical University 
      (The First People's Hospital of Shunde), Foshan 528308, Guangdong Province, PR 
      China.
FAU - Wang, Liping
AU  - Wang L
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The First Affiliated 
      Hospital of Hainan Medical University, Haikou 570102, Hainan Province, PR China.
FAU - Liu, Genglong
AU  - Liu G
AD  - Department of Pathology, The Third Affiliated Hospital of Guangdong Medical 
      University (Longjiang Hospital of Shunde District), Foshan 528318, Guangdong 
      Province, PR China.
FAU - Liu, Yayun
AU  - Liu Y
AD  - Department of Endocrinology, GuiYang Huaxi District People's Hospital. Guiyang 
      550025, Guizhou Province, PR China.
FAU - Yang, Ben
AU  - Yang B
AD  - Department of Burn Surgery, Huizhou Municipal Central Hospital, Huizhou 516000, 
      Guangdong Province, PR China.
FAU - Zhou, Zhujiang
AU  - Zhou Z
AD  - Department of Intensive Care Unit, Shunde Hospital, Southern Medical University 
      (The First People's Hospital of Shunde), Foshan 528308, Guangdong Province, PR 
      China.
FAU - Zuo, Liuer
AU  - Zuo L
AD  - Department of Intensive Care Unit, Shunde Hospital, Southern Medical University 
      (The First People's Hospital of Shunde), Foshan 528308, Guangdong Province, PR 
      China. Electronic address: 13500276597@163.com.
FAU - Chen, Zhen
AU  - Chen Z
AD  - Department of Intensive Care Unit, Shunde Hospital, Southern Medical University 
      (The First People's Hospital of Shunde), Foshan 528308, Guangdong Province, PR 
      China. Electronic address: jeanyz@foxmail.com.
LA  - eng
PT  - Journal Article
DEP - 20220307
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (Biomarkers, Tumor)
RN  - 130068-27-8 (Interleukin-10)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 14)
RN  - WI4X0X7BPJ (Hydrocortisone)
SB  - IM
MH  - Biomarkers, Tumor/genetics
MH  - Early Diagnosis
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Hydrocortisone
MH  - Immunosuppression Therapy
MH  - Interleukin-10/genetics
MH  - *Mitogen-Activated Protein Kinase 14/genetics
MH  - Prognosis
MH  - *Sepsis/diagnosis/genetics
MH  - Tumor Microenvironment
OTO - NOTNLM
OT  - Immune infiltration
OT  - Immune-related genes
OT  - Immunosuppression
OT  - Model
OT  - Sepsis
EDAT- 2022/03/11 06:00
MHDA- 2022/05/11 06:00
CRDT- 2022/03/10 20:14
PHST- 2021/12/10 00:00 [received]
PHST- 2022/01/23 00:00 [revised]
PHST- 2022/02/20 00:00 [accepted]
PHST- 2022/03/11 06:00 [pubmed]
PHST- 2022/05/11 06:00 [medline]
PHST- 2022/03/10 20:14 [entrez]
AID - S1567-5769(22)00134-5 [pii]
AID - 10.1016/j.intimp.2022.108650 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2022 Jun;107:108650. doi: 10.1016/j.intimp.2022.108650. Epub 
      2022 Mar 7.