PMID- 35276012 OWN - NLM STAT- MEDLINE DCOM- 20220524 LR - 20220716 IS - 1349-9157 (Electronic) IS - 0449-3060 (Print) IS - 0449-3060 (Linking) VI - 63 IP - 3 DP - 2022 May 18 TI - 3D reconstructed brain images reveal the possibility of the ogg1 gene to suppress the irradiation-induced apoptosis in embryonic brain in medaka (Oryzias latipes). PG - 319-330 LID - 10.1093/jrr/rrac005 [doi] AB - The accumulation of oxidative DNA lesions in neurons is associated with neurodegenerative disorders and diseases. Ogg1 (8-oxoG DNA glycosylase-1) is a primary repair enzyme to excise 7,8-dihydro-8-oxoguanine (8-oxoG), the most frequent mutagenic base lesion produced by oxidative DNA damage. We have developed ogg1-deficient medaka by screening with a high resolution melting (HRM) assay in Targeting-Induced Local Lesions In Genomes (TILLING) library. In this study, we identified that ogg1-deficient embryos have smaller brains than wild-type during the period of embryogenesis and larvae under normal conditions. To reveal the function of ogg1 when brain injury occurs during embryogenesis, we examined the induction of apoptosis in brains after exposure to gamma-rays with 10 Gy (137Cs, 7.3 Gy/min.) at 24 h post-irradiation both in wild-type and ogg1-deficient embryos. By acridine orange (AO) assay, clustered apoptosis in irradiated ogg1-deficient embryonic brains were distributed in a similar manner to those of irradiated wild-type embryos. To evaluate possible differences of gamma-ray induced apoptosis in both types of embryonic brains, we constructed 3D images of the whole brain based on serial histological sections. This analysis identified that the clustered apoptotic volume was about 3 times higher in brain of irradiated ogg1-deficient embryos (n = 3) compared to wild-type embryos (n = 3) (P = 0.04), suggesting that irradiation-induced apoptosis in medaka embryonic brain can be suppressed in the presence of functional ogg1. Collectively, reconstruction of 3D images can be a powerful approach to reveal slight differences in apoptosis induction post-irradiation. CI - (c) The Author(s) 2022. Published by Oxford University Press on behalf of The Japanese Radiation Research Society and Japanese Society for Radiation Oncology. FAU - Yasuda, Takako AU - Yasuda T AD - Laboratory of Genome Stability, Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba 277-8562, Japan. AD - Center for Environmental Risk Research, National Institute for Environmental Studies, Onogawa 16-2, Tsukuba, Ibaraki 305-8506, Japan. FAU - Li, Duolin AU - Li D AD - Laboratory of Genome Stability, Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba 277-8562, Japan. FAU - Sha, Erge AU - Sha E AD - Laboratory of Genome Stability, Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba 277-8562, Japan. FAU - Kakimoto, Fumitaka AU - Kakimoto F AD - Laboratory of Genome Stability, Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba 277-8562, Japan. FAU - Mitani, Hiroshi AU - Mitani H AD - Laboratory of Genome Stability, Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba 277-8562, Japan. FAU - Yamamoto, Hiroshi AU - Yamamoto H AD - Center for Environmental Risk Research, National Institute for Environmental Studies, Onogawa 16-2, Tsukuba, Ibaraki 305-8506, Japan. FAU - Ishikawa-Fujiwara, Tomoko AU - Ishikawa-Fujiwara T AD - Department of Radiation Biology and Medical Genetics, Graduate School of Medicine, Osaka University, B4, 2-2, Yamadaoka, Suita, Osaka 565-0871, Japan. FAU - Todo, Takeshi AU - Todo T AD - Department of Radiation Biology and Medical Genetics, Graduate School of Medicine, Osaka University, B4, 2-2, Yamadaoka, Suita, Osaka 565-0871, Japan. FAU - Oda, Shoji AU - Oda S AD - Laboratory of Genome Stability, Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba 277-8562, Japan. LA - eng GR - JP16K00541/JSPS/ PT - Journal Article PL - England TA - J Radiat Res JT - Journal of radiation research JID - 0376611 RN - 0 (Cesium Radioisotopes) RN - 4T2E65IAR7 (Cesium-137) SB - IM MH - Animals MH - Apoptosis/radiation effects MH - Brain/radiation effects MH - Cesium Radioisotopes MH - DNA Repair MH - *Oryzias PMC - PMC9124622 OTO - NOTNLM OT - 3D reconstruction OT - apoptosis OT - embryonic brain OT - irradiation OT - medaka OT - ogg1 EDAT- 2022/03/12 06:00 MHDA- 2022/05/25 06:00 PMCR- 2022/03/12 CRDT- 2022/03/11 17:11 PHST- 2021/09/24 00:00 [received] PHST- 2021/12/07 00:00 [revised] PHST- 2022/03/12 06:00 [pubmed] PHST- 2022/05/25 06:00 [medline] PHST- 2022/03/11 17:11 [entrez] PHST- 2022/03/12 00:00 [pmc-release] AID - 6547268 [pii] AID - rrac005 [pii] AID - 10.1093/jrr/rrac005 [doi] PST - ppublish SO - J Radiat Res. 2022 May 18;63(3):319-330. doi: 10.1093/jrr/rrac005.