PMID- 35276832
OWN - NLM
STAT- MEDLINE
DCOM- 20220315
LR  - 20220315
IS  - 2072-6643 (Electronic)
IS  - 2072-6643 (Linking)
VI  - 14
IP  - 3
DP  - 2022 Jan 21
TI  - Homemade Food Allergen Extracts for Use in Skin Prick Tests in the Diagnosis of 
      IgE-Mediated Food Allergy: A Good Alternative in the Absence of Commercially 
      Available Extracts?
LID - 10.3390/nu14030475 [doi]
LID - 475
AB  - INTRODUCTION: The skin prick test (SPT) is the first step in the diagnosis of an 
      immunoglobulin E (IgE)-mediated food allergy. The availability of commercial food 
      allergen extracts is very limited, resulting in a need for alternative extraction 
      methods of food allergens. The objective of this study was to compare the SPT 
      results of homemade food allergen extracts with commercially available extracts. 
      METHODS: Adult patients with a suspected food allergy were included. Food 
      allergen-specific symptoms were scored using a questionnaire. SPTs were performed 
      with homemade and commercially available extracts (ALK-Abello, Kopenhagen, 
      Denmark) from almond, apple, hazelnut, peach, peanut, and walnut. Serum-specific 
      IgE was measured with ISAC or ImmunoCAP. Intra-class correlation coefficients 
      (ICC) between the SPT results of both extract methods were calculated. The 
      proportion of agreement with food allergen-specific symptoms was analyzed. 
      RESULTS: Fifty-four patients (mean age 36; range 19-69 years; female/male: 42/12) 
      were included. The intra-class correlation coefficient (ICC) between the SPT 
      results of both extract methods were strong for hazelnut 0.79 (n = 44) and walnut 
      0.78 (n = 31), moderate for apple 0.74 (n = 21) and peanut 0.66 (n = 28), and 
      weak for almond 0.36 (n = 27) and peach 0.17 (n = 23). The proportion of 
      agreement between SPT results and food allergen-specific symptoms was comparable 
      for homemade and commercially available extracts, except for peach; 0.77 versus 
      0.36, respectively. CONCLUSION: In the diagnostic procedures to identify an 
      IgE-mediated food allergy, homemade extracts from hazelnut and walnut appear to 
      be a good alternative in the absence of commercially available food allergen 
      extracts.
FAU - Terlouw, Severina
AU  - Terlouw S
AUID- ORCID: 0000-0003-1724-3626
AD  - Department of Allergology, Albert Schweitzer Hospital, 3331 LZ Zwijndrecht, The 
      Netherlands.
AD  - Internal Medicine, Allergology & Clinical Immunology, Erasmus MC, University 
      Medical Centre, 3015 GD Rotterdam, The Netherlands.
FAU - van Boven, Frank E
AU  - van Boven FE
AD  - Internal Medicine, Allergology & Clinical Immunology, Erasmus MC, University 
      Medical Centre, 3015 GD Rotterdam, The Netherlands.
FAU - Borsboom-van Zonneveld, Monika
AU  - Borsboom-van Zonneveld M
AD  - Department of Allergology, Albert Schweitzer Hospital, 3331 LZ Zwijndrecht, The 
      Netherlands.
FAU - de Graaf-In 't Veld, Catharina
AU  - de Graaf-In 't Veld C
AD  - Department of Allergology, Albert Schweitzer Hospital, 3331 LZ Zwijndrecht, The 
      Netherlands.
FAU - van Splunter, Marloes E
AU  - van Splunter ME
AD  - Internal Medicine, Allergology & Clinical Immunology, Erasmus MC, University 
      Medical Centre, 3015 GD Rotterdam, The Netherlands.
FAU - van Daele, Paul L A
AU  - van Daele PLA
AD  - Internal Medicine, Allergology & Clinical Immunology, Erasmus MC, University 
      Medical Centre, 3015 GD Rotterdam, The Netherlands.
FAU - van Maaren, Maurits S
AU  - van Maaren MS
AD  - Internal Medicine, Allergology & Clinical Immunology, Erasmus MC, University 
      Medical Centre, 3015 GD Rotterdam, The Netherlands.
FAU - Schreurs, Marco W J
AU  - Schreurs MWJ
AD  - Laboratory Medical Immunology, Department of Immunology, Erasmus Medical Centre, 
      3015 GD Rotterdam, The Netherlands.
FAU - de Jong, Nicolette W
AU  - de Jong NW
AD  - Internal Medicine, Allergology & Clinical Immunology, Erasmus MC, University 
      Medical Centre, 3015 GD Rotterdam, The Netherlands.
AD  - Department of Pediatric Allergology, Sophia Children Hospital, Erasmus Medical 
      Centre, 3015 GD Rotterdam, The Netherlands.
LA  - eng
PT  - Journal Article
DEP - 20220121
PL  - Switzerland
TA  - Nutrients
JT  - Nutrients
JID - 101521595
RN  - 0 (Allergens)
RN  - 0 (Plant Extracts)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Allergens
MH  - Female
MH  - *Food Hypersensitivity/diagnosis
MH  - Humans
MH  - *Immunoglobulin E
MH  - Male
MH  - Middle Aged
MH  - Plant Extracts
MH  - Skin Tests/methods
MH  - Young Adult
PMC - PMC8839075
OTO - NOTNLM
OT  - diagnosis
OT  - extracts
OT  - food allergy
OT  - oral allergy syndrome
OT  - skin prick test
OT  - specific IgE
COIS- The authors declare no conflict of interest.
EDAT- 2022/03/13 06:00
MHDA- 2022/03/16 06:00
PMCR- 2022/01/21
CRDT- 2022/03/12 01:00
PHST- 2021/12/17 00:00 [received]
PHST- 2022/01/14 00:00 [revised]
PHST- 2022/01/19 00:00 [accepted]
PHST- 2022/03/12 01:00 [entrez]
PHST- 2022/03/13 06:00 [pubmed]
PHST- 2022/03/16 06:00 [medline]
PHST- 2022/01/21 00:00 [pmc-release]
AID - nu14030475 [pii]
AID - nutrients-14-00475 [pii]
AID - 10.3390/nu14030475 [doi]
PST - epublish
SO  - Nutrients. 2022 Jan 21;14(3):475. doi: 10.3390/nu14030475.