PMID- 35278152
OWN - NLM
STAT- MEDLINE
DCOM- 20220425
LR  - 20221005
IS  - 1559-0283 (Electronic)
IS  - 1085-9195 (Linking)
VI  - 80
IP  - 2
DP  - 2022 Jun
TI  - MicroRNA-629-3p Promotes Interleukin-13-Induced Bronchial Epithelial Cell Injury 
      and Inflammation by Targeting FOXA2.
PG  - 457-466
LID - 10.1007/s12013-022-01072-6 [doi]
AB  - OBJECTIVE: Asthma is a chronic pulmonary inflammatory disease. MicroRNA 
      (miR)-629-3p expression is reported to be up-regulated in the sputum of asthma 
      patients. Nonetheless, miR-629-3p's role and mechanism in asthma remain largely 
      unknown. This study is aimed at exploring miR-629-3p's role in regulating the 
      injury and inflammation of bronchial epithelial cells. METHODS: Quantitative 
      real-time polymerase chain reaction (qRT-PCR) was conducted to detect the 
      expression levels of miR-629-3p and forkhead box a2 (FOXA2) mRNA in 16HBE cells 
      treated with interleukin-13 (IL-13). 16HBE cell viability was evaluated using the 
      cell counting kit-8 (CCK-8) assay, and cell apoptosis was analyzed by a flow 
      cytometer. The levels of C-C motif chemokine ligand 11 (CCL11), C-C motif 
      chemokine ligand 26 (CCL26), C-C motif ligand 2 (CCL-2)/mono-cyte chemotactic 
      protein-1 (MCP-1), interleukin-1 beta (IL-1b), and interleukin 6 (IL-6) in 16HBE 
      cell supernatant were detected through enzyme-linked immunosorbent assay (ELISA). 
      The downstream target genes of miR-629-3p were predicted through bioinformatics. 
      Besides, the targeted relationship between miR-629-3p and FOXA2 mRNA 3'-UTR was 
      verified by dual-luciferase reporter gene assay. Western blot was utilized to 
      determine the regulatory effects of miR-629-3p on the expression of FOXA2 protein 
      in 16HBE cells. RESULTS: MiR-629-3p expression was significantly enhanced in 
      IL-13-stimulated 16HBE cells while the FOXA2 mRNA and protein levels were 
      significantly down-regulated. The transfection of miR-629-3p mimics inhibited 
      16HBE cells' viability, and promoted the apoptosis and the secretion of 
      chemokines CCL11, CCL26, CCL-2/MCP-1, IL-1b, and IL-6 of 16HBE cells, whereas 
      inhibiting miR-629-3p had the opposite effects. Moreover, FOXA2 was identified as 
      a downstream miR-629-3p target, and its overexpression reversed the effects of 
      the miR-629-3p on 16HBE cells. CONCLUSION: MiR-629-3p promotes IL-13-induced 
      16HBE cells' injury and inflammation by targeting FOXA2.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Jian, Guo
AU  - Jian G
AD  - Department of Intensive Care Unit, The Lihuili Affiliated Hospital, Ningbo 
      University, Ningbo, Zhejiang, China.
FAU - Yangli, Jin
AU  - Yangli J
AD  - Department of Ultrasound, Yinzhou No. 2 Hospital, Ningbo, Zhejiang, China. 
      553814675@qq.com.
FAU - Chao, Zhang
AU  - Chao Z
AD  - Department of Pediatrics, Xi'an No. 3 Hospital, Xi'an, Shaanxi, China.
FAU - Kun, Wang
AU  - Kun W
AD  - Department of Pediatrics, Xi'an No. 3 Hospital, Xi'an, Shaanxi, China.
FAU - Xiaomin, Zhang
AU  - Xiaomin Z
AUID- ORCID: 0000-0001-9146-2849
AD  - Department of Pediatrics, Xi'an No. 3 Hospital, Xi'an, Shaanxi, China. 
      drzhangxm@21cn.com.
LA  - eng
PT  - Journal Article
DEP - 20220312
PL  - United States
TA  - Cell Biochem Biophys
JT  - Cell biochemistry and biophysics
JID - 9701934
RN  - 0 (Chemokines)
RN  - 0 (FOXA2 protein, human)
RN  - 0 (Interleukin-13)
RN  - 0 (Interleukin-6)
RN  - 0 (Ligands)
RN  - 0 (MIRN629 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Messenger)
RN  - 135845-92-0 (Hepatocyte Nuclear Factor 3-beta)
SB  - IM
MH  - Apoptosis/genetics
MH  - *Asthma/metabolism
MH  - Chemokines/adverse effects/metabolism
MH  - Epithelial Cells/metabolism
MH  - Hepatocyte Nuclear Factor 3-beta/genetics/metabolism
MH  - Humans
MH  - Inflammation/chemically induced/genetics/metabolism
MH  - Interleukin-13/adverse effects/pharmacology
MH  - Interleukin-6/metabolism
MH  - Ligands
MH  - *MicroRNAs/genetics/metabolism
MH  - RNA, Messenger/genetics/metabolism
OTO - NOTNLM
OT  - Asthma
OT  - Cell damage
OT  - FOXA2
OT  - Inflammation
OT  - MiR-629-3p
EDAT- 2022/03/13 06:00
MHDA- 2022/04/26 06:00
CRDT- 2022/03/12 12:07
PHST- 2021/05/11 00:00 [received]
PHST- 2022/02/14 00:00 [accepted]
PHST- 2022/03/13 06:00 [pubmed]
PHST- 2022/04/26 06:00 [medline]
PHST- 2022/03/12 12:07 [entrez]
AID - 10.1007/s12013-022-01072-6 [pii]
AID - 10.1007/s12013-022-01072-6 [doi]
PST - ppublish
SO  - Cell Biochem Biophys. 2022 Jun;80(2):457-466. doi: 10.1007/s12013-022-01072-6. 
      Epub 2022 Mar 12.