PMID- 35278290
OWN - NLM
STAT- MEDLINE
DCOM- 20220315
LR  - 20220315
IS  - 1426-9686 (Print)
IS  - 1426-9686 (Linking)
VI  - 50
IP  - 295
DP  - 2022 Feb 22
TI  - Dental status and oxidative homeostasis state in patients exposed to occupational 
      vibration: reduced glutathione content in oral fluid.
PG  - 9-12
AB  - AIM: The aim of the research involved determination of reduced glutathione 
      content in oral fluid of patients exposed to occupational vibration depending 
      upon their dental status. MATERIALS AND METHODS: The assessment of dental status 
      (DS) and reduced glutathione (RG) content in oral fluid (OF) was carried out in 
      three groups of patients: control group (n=129) included the persons exposed to 
      occupational vibration whose results of comprehensive medical examination 
      excluded the presence of vibration disease (VD); the second group (n=63 patients 
      with the VD stage I) and the third group (n=66 patients with VD stage II), who 
      underwent treatment at the clinical department of the Research Institute of 
      Occupational Hygiene and Occupational Diseases of Kharkiv National Medical 
      University of the Ministry of Health of Ukraine. DS determination was carried out 
      according to the method of K. M. Kosenko (Patent No. 57512, Ukraine) for 
      in-patients and controls (during medical checkups) using the following indices: 
      PMA, OHI-S, DMFT, with assessment of vacuum-pressory resistance of gingival 
      capillaries (VPRC) (according to V. I. Kulazhenko) and community periodontal 
      index of treatment needs (CPITN). RG content (activity) in OF was determined 
      according to Garishvili. Primary data were statistically processed with the 
      determination of accuracy by Student's test. RESULTS: Assessment of metabolic 
      indices, which characterize the state of oxidative homeostasis enzyme chain, 
      showed that RG content in OF depending upon VD severity reliably (p<0.05) 
      reduced. RG content depending upon PMA intensity in patients with VD ranged from 
      23.5+/-0.8 mg/cm3 to 28.6+/-0.3 U/min and was reliably (p<0.05) lower in patients 
      with VD stage I versus controls (23.2+/-1.0 U/min and 26.7+/-0.3 U/min respectively, 
      when PMA>2.1) and also reliably lower in patients with VD stage II versus 
      patients with VD stage I (29.9+/-0.9 U/min and 26.2+/-0.4 U/min respectively, when 
      PMA>1.0). RG content depending upon OHI-S values in patients with VD ranged from 
      33.4+/-1.2 U/min to 24.1+/-1.1 U/min and was reliably (d<0.05) lower in patients with 
      OHI-S values equal to 1.7 U and higher versus controls (24.1+/-1.1 U/min and 
      27.1+/-0.2 U/min, respectively) and also reliably lower in patients with VD stage 
      II versus patients with VD stage I (27.3+/-0.4 U/ min and 33.4+/-1.2 U/min, 
      respectively, with OHI-S</=0.6 U). A comparative analysis showed that the activity 
      of the enzymatic protection of the periodontal membrane could be also determined 
      by the state of hard tissues, in particular by such DS index as DFMT. The 
      activity of RG in VD stage I was shown to be reliably (p<0.05) reduced in 
      patients with DFMT index exceeding 15 pts (in DFMT</=10 pts RG activity was 
      32.2+/-0.4 U/min, whereas in DFMT exceeding 15 pts it was equal to 26.7+/-0.6 U/min). 
      Somewhat different pattern of RG activity in OF was found in patients with VD 
      stage II: RG activity in OF was reduced in all DFMT values in these patients and 
      its reduction was shown to be dependent on an increase in DMFT index. In DMFT</=10 
      the patients with VD stage II were found to have a reliable (p<0.05) reduction in 
      RG activity in OF versus the control group (30.1+/-0.3 U/ min and 23.8+/-0.5 U/min, 
      respectively) and this activity was shown to be inhibited in DFMT increase 
      (23.8+/-0.5 U/min with DFMT</=10 and 19.3+/-0.9 U/min with DFMT>/=20 pts, respectively). 
      RG content depending upon VPRC in VD patients ranged from 29.2+/-0.1 U/min to 
      29.2+/-0.1 U/min and was reliably (p<0.05) lower in patients with their VPRC values 
      </=40 sec. Assessment of RG activity in OF of VD patients with different levels of 
      CPITN showed that RG content in persons requiring comprehensive treatment (also 
      including prosthetic treatment; CPITN>/=3.1 pts) was reliably reduced (versus 
      corresponding groups of patients but with low CPITN values) both in VD stages I 
      and II (24.1+/-1.0 U/min and 19.3+/-0.9 U/min, respectively). CONCLUSIONS: Increases 
      in the rate and expression of periodontal lesions (by PMA index) depending upon 
      the presence and severity of VD with a proper decrease in the level of RG content 
      in OF were registered. An activation of the enzymatic chain of antioxidant 
      protection in patients with VD under low HI values and a simultaneous inhibition 
      of enzymatic activity under high HI values were found out. VD stage I revealed an 
      increased RG activity versus controls (p<0.05), while VD stage II demonstrated a 
      reliable (p<0.05) reduction of the above activity. Moreover, an unsatisfactory 
      state of the oral cavity hygiene contributed to enzymatic protection of their 
      periodontium in patients with VD (irrespective of its stage). Regularities were 
      revealed, which supported the benefit of pathogenetic relationships between the 
      state of the periodontal microcirculatory bed and enzymatic activity of OF in 
      patients with VD. Both patients with VD and persons, who are exposed to 
      occupational vibration, need for diagnosis of activity of enzymes in OF since, as 
      our analysis of findings has shown, DS indices are interdependent with activity 
      of the enzymatic chain of the antioxidant protection of OF.
CI  - (c) 2022 MEDPRESS.
FAU - Cherkashyna, Lidiya
AU  - Cherkashyna L
AD  - Kharkiv Medical Academy of Postgraduate Education, Ukraine.
FAU - Haidash, Ihor
AU  - Haidash I
AD  - Lugansk State Medical University, Ukraine.
FAU - Shabelnik, Oleh
AU  - Shabelnik O
AD  - Lugansk State Medical University, Ukraine.
FAU - Shklyar, Anton
AU  - Shklyar A
AD  - Kharkiv National Medical University, Ukraine.
FAU - Sukhonosov, Roman
AU  - Sukhonosov R
AD  - Kharkiv National Medical University, Ukraine.
FAU - Barchan, Ganna
AU  - Barchan G
AD  - Kharkiv National Medical University, Ukraine.
FAU - Lakhtin, Yurii
AU  - Lakhtin Y
FAU - Demikhova, Nadiia
AU  - Demikhova N
AD  - Sumy State University, Ukraine.
FAU - Orlovskiy, Aleksandr
AU  - Orlovskiy A
AD  - Sumy State University, Ukraine.
LA  - eng
PT  - Journal Article
PL  - Poland
TA  - Pol Merkur Lekarski
JT  - Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego
JID - 9705469
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - *Glutathione
MH  - Homeostasis
MH  - Humans
MH  - Microcirculation
MH  - Oxidative Stress
MH  - *Vibration/adverse effects
OTO - NOTNLM
OT  - dental status
OT  - periodontal membrane
OT  - reduced glutathione
OT  - vibration disease
EDAT- 2022/03/13 06:00
MHDA- 2022/03/16 06:00
CRDT- 2022/03/12 17:03
PHST- 2022/03/12 17:03 [entrez]
PHST- 2022/03/13 06:00 [pubmed]
PHST- 2022/03/16 06:00 [medline]
AID - PML295-009 [pii]
PST - ppublish
SO  - Pol Merkur Lekarski. 2022 Feb 22;50(295):9-12.