PMID- 35279089 OWN - NLM STAT- MEDLINE DCOM- 20220321 LR - 20220322 IS - 1471-2229 (Electronic) IS - 1471-2229 (Linking) VI - 22 IP - 1 DP - 2022 Mar 12 TI - Molecular cytogenetics and development of St-chromosome-specific molecular markers of novel stripe rust resistant wheat-Thinopyrum intermedium and wheat-Thinopyrum ponticum substitution lines. PG - 111 LID - 10.1186/s12870-022-03496-x [doi] LID - 111 AB - BACKGROUND: Owing to their excellent resistance to abiotic and biotic stress, Thinopyrum intermedium (2n = 6x = 42, JJJ(s)J(s)StSt) and Th. ponticum (2n = 10x = 70) are both widely utilized in wheat germplasm innovation programs. Disomic substitution lines (DSLs) carrying one pair of alien chromosomes are valuable bridge materials for transmission of novel genes, fluorescence in situ hybridization (FISH) karyotype construction and specific molecular marker development. RESULTS: Six wheat-Thinopyrum DSLs derived from crosses between Abbondanza nullisomic lines (2n = 40) and two octoploid Trititrigia lines (2n = 8x = 56), were characterized by sequential FISH-genome in situ hybridization (GISH), multicolor GISH (mc-GISH), and an analysis of the wheat 15 K SNP array combined with molecular marker selection. ES-9 (DS2St (2A)) and ES-10 (DS3St (3D)) are wheat-Th. ponticum DSLs, while ES-23 (DS2St (2A)), ES-24 (DS3St (3D)), ES-25(DS2St (2B)), and ES-26 (DS2St (2D)) are wheat-Th. intermedium DSLs. ES-9, ES-23, ES-25 and ES-26 conferred high thousand-kernel weight and stripe rust resistance at adult stages, while ES-10 and ES-24 were highly resistant to stripe rust at all stages. Furthermore, cytological analysis showed that the alien chromosomes belonging to the same homoeologous group (2 or 3) derived from different donors carried the same FISH karyotype and could form a bivalent. Based on specific-locus amplified fragment sequencing (SLAF-seq), two 2St-chromosome-specific markers (PTH-005 and PTH-013) and two 3St-chromosome-specific markers (PTH-113 and PTH-135) were developed. CONCLUSIONS: The six wheat-Thinopyrum DSLs conferring stripe rust resistance can be used as bridging parents for transmission of valuable resistance genes. The utility of PTH-113 and PTH-135 in a BC1F2 population showed that the newly developed markers could be useful tools for efficient identification of St chromosomes in a common wheat background. CI - (c) 2022. The Author(s). FAU - Wang, Siwen AU - Wang S AD - College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, China. FAU - Wang, Changyou AU - Wang C AD - College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, China. AD - Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling, 712100, Shaanxi, China. AD - State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling, 712100, Shaanxi, China. FAU - Feng, Xianbo AU - Feng X AD - College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, China. FAU - Zhao, Jixin AU - Zhao J AD - College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, China. AD - Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling, 712100, Shaanxi, China. AD - State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling, 712100, Shaanxi, China. FAU - Deng, Pingchuan AU - Deng P AD - College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, China. AD - Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling, 712100, Shaanxi, China. AD - State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling, 712100, Shaanxi, China. FAU - Wang, Yajuan AU - Wang Y AD - College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, China. AD - Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling, 712100, Shaanxi, China. AD - State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling, 712100, Shaanxi, China. FAU - Zhang, Hong AU - Zhang H AD - College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, China. AD - Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling, 712100, Shaanxi, China. AD - State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling, 712100, Shaanxi, China. FAU - Liu, Xinlun AU - Liu X AD - College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, China. AD - Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling, 712100, Shaanxi, China. AD - State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling, 712100, Shaanxi, China. FAU - Li, Tingdong AU - Li T AD - College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, China. AD - Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling, 712100, Shaanxi, China. AD - State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling, 712100, Shaanxi, China. FAU - Chen, Chunhuan AU - Chen C AD - College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, China. AD - Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling, 712100, Shaanxi, China. AD - State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling, 712100, Shaanxi, China. FAU - Wang, Baotong AU - Wang B AD - State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling, 712100, Shaanxi, China. AD - College of Plant Protection, Northwest A&F University, Yangling, 712100, Shaanxi, China. FAU - Ji, Wanquan AU - Ji W AD - College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, China. jiwanquan2008@126.com. AD - Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling, 712100, Shaanxi, China. jiwanquan2008@126.com. AD - State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling, 712100, Shaanxi, China. jiwanquan2008@126.com. LA - eng PT - Comparative Study PT - Journal Article DEP - 20220312 PL - England TA - BMC Plant Biol JT - BMC plant biology JID - 100967807 RN - 0 (Genetic Markers) SB - IM MH - *Chromosomes, Plant MH - Cytogenetic Analysis MH - Disease Resistance/*genetics MH - *Genetic Markers MH - Genetic Variation MH - Genotype MH - Poaceae/*genetics/*microbiology MH - Puccinia/*pathogenicity MH - Triticum/*genetics/*microbiology PMC - PMC8917741 OTO - NOTNLM OT - St-chromosome-specific molecular markers OT - Stripe rust resistance OT - Substitution lines OT - Thinopyrum intermedium OT - Thinopyrum ponticum COIS- The authors declare that they have no conflict of interests. EDAT- 2022/03/14 06:00 MHDA- 2022/03/22 06:00 PMCR- 2022/03/12 CRDT- 2022/03/13 20:22 PHST- 2021/09/16 00:00 [received] PHST- 2022/02/24 00:00 [accepted] PHST- 2022/03/13 20:22 [entrez] PHST- 2022/03/14 06:00 [pubmed] PHST- 2022/03/22 06:00 [medline] PHST- 2022/03/12 00:00 [pmc-release] AID - 10.1186/s12870-022-03496-x [pii] AID - 3496 [pii] AID - 10.1186/s12870-022-03496-x [doi] PST - epublish SO - BMC Plant Biol. 2022 Mar 12;22(1):111. doi: 10.1186/s12870-022-03496-x.