PMID- 35280398
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220315
IS  - 2305-5839 (Print)
IS  - 2305-5847 (Electronic)
IS  - 2305-5839 (Linking)
VI  - 10
IP  - 4
DP  - 2022 Feb
TI  - Polyinosinic:polycytidylic acid aggravates calcipotriol-induced atopic 
      dermatitis-like skin lesions in mice by increasing the expression of thymic 
      stromal lymphopoietin.
PG  - 209
LID - 10.21037/atm-22-282 [doi]
LID - 209
AB  - BACKGROUND: Polyinosinic:polycytidylic acid [poly (I:C)] is a synthetic viral 
      double-stranded RNA analog that can activate Toll-like receptor 3 (TLR3) and 
      induce the release of thymic stromal lymphopoietin (TSLP). TSLP has been shown to 
      contribute to atopic dermatitis (AD). This study explored the effects of poly 
      (I:C) in a calcipotriol-induced model of murine AD. METHODS: Calcipotriol (MC903) 
      was used to establish AD-like mice model. Mice in the MC903 + poly (I:C) group 
      were then treated with poly (I:C) in a concentration of 5 microg/g bodyweight. The 
      impact of poly (I:C) treatment on these animals was assessed based upon changes 
      in lesions, bodyweight, ear thickness, and histopathological findings. In 
      addition, serum interleukin 4 (IL-4), interferon-gamma (IFN-gamma), immunoglobulin E 
      (IgE), IL-13, and TSLP levels were measured using enzyme-linked immunosorbent 
      assay (ELISA), while tissue IL-13 and TSLP levels were assessed using ELISA, 
      Western blotting, and immunohistochemical staining, and mast cell infiltration 
      was assessed through toluidine blue (TBO) staining. RESULTS: Relative to vehicle 
      control treatment, poly (I:C) administration was associated with a significant 
      exacerbation of calcipotriol-induced AD-like murine skin lesions. In animals 
      treated with poly (I:C), the levels of serum IL-4, IL-13 and TSLP increased 
      significantly, while the level of IFN-gamma did not change. It also increased IL-13 
      and TSLP levels in skin lesions relative to the control-group mice and increased 
      dermal mast cell infiltration and IgE production. CONCLUSIONS: These data 
      indicate that poly (I:C) treatment and exogenous activation of TLR3 exacerbate 
      murine calcipotriol-induced AD-like skin lesions in part by increasing the 
      production of TSLP and other T-helper 2 (Th2)-related cytokines. KEYWORDS: Atopic 
      dermatitis (AD); polyinosinic:polycytidylic acid [poly (I:C)]; thymic stromal 
      lymphopoietin (TSLP); Toll-like receptor 3 (TLR3).
CI  - 2022 Annals of Translational Medicine. All rights reserved.
FAU - Wan, Haoyue
AU  - Wan H
AD  - Department of Dermatology, Nanjing First Hospital, Nanjing Medical University, 
      Nanjing, China.
FAU - Yang, Huixue
AU  - Yang H
AD  - Department of Dermatology, Nanjing First Hospital, Nanjing Medical University, 
      Nanjing, China.
FAU - Wei, Mingjing
AU  - Wei M
AD  - Department of Dermatology, Nanjing First Hospital, Nanjing Medical University, 
      Nanjing, China.
FAU - Chen, Wenqi
AU  - Chen W
AD  - Department of Dermatology, Nanjing First Hospital, Nanjing Medical University, 
      Nanjing, China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Ann Transl Med
JT  - Annals of translational medicine
JID - 101617978
PMC - PMC8908153
COIS- Conflicts of Interest: All authors have completed the ICMJE uniform disclosure 
      form (available at 
      https://atm.amegroups.com/article/view/10.21037/atm-22-282/coif). All authors 
      report funding from Nanjing Medical Science and Technology Development Project 
      (Grant No. YKK16134). The authors have no other conflicts of interest to declare.
EDAT- 2022/03/15 06:00
MHDA- 2022/03/15 06:01
PMCR- 2022/02/01
CRDT- 2022/03/14 05:10
PHST- 2021/12/22 00:00 [received]
PHST- 2022/02/18 00:00 [accepted]
PHST- 2022/03/14 05:10 [entrez]
PHST- 2022/03/15 06:00 [pubmed]
PHST- 2022/03/15 06:01 [medline]
PHST- 2022/02/01 00:00 [pmc-release]
AID - atm-10-04-209 [pii]
AID - 10.21037/atm-22-282 [doi]
PST - ppublish
SO  - Ann Transl Med. 2022 Feb;10(4):209. doi: 10.21037/atm-22-282.