PMID- 35280407
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220830
IS  - 2305-5839 (Print)
IS  - 2305-5847 (Electronic)
IS  - 2305-5839 (Linking)
VI  - 10
IP  - 4
DP  - 2022 Feb
TI  - Mechanism of protective role of miR-874-3p in intervertebral disc degeneration.
PG  - 213
LID - 10.21037/atm-22-91 [doi]
LID - 213
AB  - BACKGROUND: The intervertebral disc can increase the amplitude of spinal motion, 
      withstand pressure, buffer vibration, and protect the brain and spinal cord. It 
      is also the main reason why height changes, but the regulatory mechanism is still 
      unclear, and this study mainly explored the role of miR-874-3p in intervertebral 
      disc degeneration (IDD). METHODS: The mechanism and perform correlation analysis 
      of miR-874-3p and the pathological degree and prognosis of patients with IDD. 
      miR-874-3p is involved in the progression of several diseases, such as cell 
      differentiation, proliferation, apoptosis and extracellular matrix degradation, 
      overexpressing cell line GV369-miR-874-3p-NP was obtained by infected nucleus 
      pulposus (NP) cells, and the empty vector GV369-NP group was set with the blank 
      group. The expression of the tag protein (green fluorescent protein, GFP) was 
      visualized by fluorescence microscopy, followed by real-time polymerase chain 
      reaction (PCR) method to detect miR-874-3p expression, apoptosis by flow 
      cytometry, luciferase reporter analysis for verifying the targeting relationship 
      between miR-874-3p, caspase-3, B-cell lymphoma-2 (Bcl-2) and Bax in cells, and 
      examined the changes in cellular mitochondrial membrane potential using kits. 
      RESULTS: The expression of miR-874-3p was significantly reduced in IDD patients, 
      and was negatively correlated with matrix metallopeptidase 2 (MMP2) and 
      downregulated matrix metallopeptidase 3 (MMP3) in the NP cells. In addition, 
      western blot revealed that overexpression of miR-874-3p increased the aggregation 
      protein level in the NP cells. CONCLUSIONS: miR-874-3p can inhibit the cell death 
      of IDD, and not only participate in caspase-3 and Fas-associating protein with a 
      novel death domain (FADD)-mediated apoptosis through targeted regulation of 
      exogenous MMP2/MMP3 pathway, but also play a role in cell apoptosis through 
      mitochondrial pathway.
CI  - 2022 Annals of Translational Medicine. All rights reserved.
FAU - Yang, Xuefang
AU  - Yang X
AD  - Orthopedics Department, Yingkou Central Hospital, Yingkou, China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Ann Transl Med
JT  - Annals of translational medicine
JID - 101617978
PMC - PMC8908150
OTO - NOTNLM
OT  - cell apoptosis
OT  - intervertebral disc degeneration (IDD)
OT  - miR-874-3p
OT  - nucleus pulposus cells
COIS- Conflicts of Interest: The author has completed the ICMJE uniform disclosure form 
      (available at https://atm.amegroups.com/article/view/10.21037/atm-22-91/coif). 
      The author has no conflicts of interest to declare.
EDAT- 2022/03/15 06:00
MHDA- 2022/03/15 06:01
PMCR- 2022/02/01
CRDT- 2022/03/14 05:10
PHST- 2021/10/22 00:00 [received]
PHST- 2022/02/21 00:00 [accepted]
PHST- 2022/03/14 05:10 [entrez]
PHST- 2022/03/15 06:00 [pubmed]
PHST- 2022/03/15 06:01 [medline]
PHST- 2022/02/01 00:00 [pmc-release]
AID - atm-10-04-213 [pii]
AID - 10.21037/atm-22-91 [doi]
PST - ppublish
SO  - Ann Transl Med. 2022 Feb;10(4):213. doi: 10.21037/atm-22-91.